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Western Blot (WB) (Mouse Src Antibody (N-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Src antibody detected the Src protein (arrow).)

Rabbit anti-Mouse Src Polyclonal Antibody | anti-Src antibody

Mouse Src Antibody (N-term)

Gene Names
Src; AW259666; pp60c-src
Reactivity
Mouse
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Src; Polyclonal Antibody; Mouse Src Antibody (N-term); Neuronal proto-oncogene tyrosine-protein kinase Src; Proto-oncogene c-Src; pp60c-src; p60-Src; anti-Src antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Mouse Src antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-125 amino acids from the N-terminal region of mouse Src.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
98-125
Sequence Length
535
Applicable Applications for anti-Src antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
MOUSE
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Mouse Src Antibody (N-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Src antibody detected the Src protein (arrow).)

Western Blot (WB) (Mouse Src Antibody (N-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Src antibody detected the Src protein (arrow).)
Related Product Information for anti-Src antibody
Non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, plays an important role in osteoclastic bone resorption. Both the formation of a SRC-PTK2B complex, and SRC kinase activity are necessary for this function. Once it is recruited to the activated integrins, by PTK2B, it phosphorylates CBL which in turn induces the activation and recruitment of phosphatidylinositol 3-kinase to the cell membrane in a signaling pathway that is critical for osteoclast function. Promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. Phosphorylates RUNX3 and COX2 on tyrosine residues, TNK2 on 'Tyr-284' and CBL on 'Tyr-731'. Enhances DDX58/RIG-I-elicited antiviral signaling.
Product Categories/Family for anti-Src antibody
References
Tanji, M., et al. Mol. Cell. Biol. 30(19):4604-4615(2010)
Wang, L., et al. J. Neurosci. 30(39):13201-13210(2010)
Tu, C., et al. Proc. Natl. Acad. Sci. U.S.A. 107(37):16107-16112(2010)
Zheng, Y., et al. Mol. Cell. Biol. 30(17):4280-4292(2010)
Bershteyn, M., et al. Dev. Cell 19(2):270-283(2010)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
60645
NCBI Official Full Name
neuronal proto-oncogene tyrosine-protein kinase Src isoform 2
NCBI Official Synonym Full Names
Rous sarcoma oncogene
NCBI Official Symbol
Src
NCBI Official Synonym Symbols
AW259666; pp60c-src
NCBI Protein Information
neuronal proto-oncogene tyrosine-protein kinase Src
UniProt Protein Name
Neuronal proto-oncogene tyrosine-protein kinase Src
Protein Family
UniProt Gene Name
Src
UniProt Synonym Gene Names
p60-Src
UniProt Entry Name
SRC_MOUSE

Uniprot Description

Src: proto-oncogenic cytoplasmic tyrosine kinase of the SRC family. Highly expressed in certain fully differentiated cells such as neurons, platelets and macrophages. Phosphorylation of an activation loop tyrosine activates the enzyme; phosphorylation of a tyrosine in the C-terminus by Csk inhibits the enzyme. Two alternatively spliced isoforms have been described.

Protein type: Kinase, protein; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Protein kinase, TK; Oncoprotein; TK group; Src family

Cellular Component: extrinsic to internal side of plasma membrane; cytoskeleton; mitochondrion; membrane; perinuclear region of cytoplasm; mitochondrial inner membrane; cytoplasm; plasma membrane; actin filament; caveola; intracellular; nucleus

Molecular Function: transferase activity; protein domain specific binding; protein binding; ephrin receptor binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; non-membrane spanning protein tyrosine kinase activity; kinase activity; ATP binding; protein kinase activity; hormone receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; oogenesis; regulation of estrogen receptor signaling pathway; cell migration; progesterone receptor signaling pathway; peptidyl-tyrosine phosphorylation; regulation of cell cycle; immune system process; platelet-derived growth factor receptor signaling pathway; cell cycle; uterus development; protein amino acid phosphorylation; regulation of cell proliferation; regulation of protein binding; forebrain development; innate immune response; cell adhesion; phosphorylation; bone resorption

Research Articles on Src

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Product Notes

The Src src (Catalog #AAA9201687) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 98-125. The Mouse Src Antibody (N-term) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Src can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the Src src for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Src, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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