Sprouty Homolog 2 (SPRY2) Recombinant Protein | SPRY2 recombinant protein

Recombinant Sprouty Homolog 2 (SPRY2)

Cat. Num. AAA2018432

• Gene Names: SPRY2; hSPRY2
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Sprouty Homolog 2 (SPRY2); Recombinant Sprouty Homolog 2 (SPRY2); SPRY2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SGSRS STRTSTSSSS SEQRLLGSSF SSGPVADGII RVQPKSELKP GELKPLSKED LGLHAYRCED CGKCKCKECT YPRPLPSDWI CDKQCLCSAQ NVIDYGTCVC CVKGLFYHCS NDDEDNCADN PCSCSQSHCC TRWSAMGVMS LFLPCLWCYL PAKGCLKLCQ GCYDRVNRPG CRCKNSNTVC CKVPT
• Sequence Length: 315

• Applicable Applications for SPRY2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Ser116~Thr305 (Accession # O43597) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 5032115
• NCBI GeneID: 10253
• NCBI Accession #: NP_005833.1
• NCBI GenBank Nucleotide #: NM_005842.2
• UniProt Accession #: O43597; Q5T6Z7
• Molecular Weight: 24.6kDa
• NCBI Official Full Name: protein sprouty homolog 2
• NCBI Official Synonym Full Names: sprouty homolog 2 (Drosophila)
• NCBI Official Symbol: SPRY2
• NCBI Official Synonym Symbols: hSPRY2
• NCBI Protein Information: protein sprouty homolog 2; spry-2; sprouty 2
• UniProt Protein Name: Protein sprouty homolog 2
• Protein Family: Protein sprouty
• UniProt Gene Name: SPRY2
• UniProt Synonym Gene Names: Spry-2
• UniProt Entry Name: SPY2_HUMAN

NCBI Description

This gene encodes a protein belonging to the sprouty family. The encoded protein contains a carboxyl-terminal cysteine-rich domain essential for the inhibitory activity on receptor tyrosine kinase signaling proteins and is required for growth factor stimulated translocation of the protein to membrane ruffles. In primary dermal endothelial cells this gene is transiently upregulated in response to fibroblast growth factor two. This protein is indirectly involved in the non-cell autonomous inhibitory effect on fibroblast growth factor two signaling. The protein interacts with Cas-Br-M (murine) ectropic retroviral transforming sequence, and can function as a bimodal regulator of epidermal growth factor receptor/mitogen-activated protein kinase signaling. This protein may play a role in alveoli branching during lung development as shown by a similar mouse protein. [provided by RefSeq, Jul 2008]

Uniprot Description

SPRY2: a feedback inhibitor of growth factor receptor signaling. Belongs to the sprouty family. May function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis. Associated with microtubules in unstimulated cells but is translocated to the membrane ruffles in cells stimulated with EGF (epidermal growth factor).

Protein type: Adaptor/scaffold; Inhibitor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 13q31.1

Cellular Component: microtubule cytoskeleton; microtubule; plasma membrane; cytosol

Molecular Function: protein serine/threonine kinase inhibitor activity; protein serine/threonine kinase activator activity; protein binding; protein kinase binding

Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of epidermal growth factor receptor signaling pathway; negative regulation of MAP kinase activity; inner ear morphogenesis; fibroblast growth factor receptor signaling pathway; cell fate commitment; negative regulation of nerve growth factor receptor signaling pathway; negative regulation of fibroblast growth factor receptor signaling pathway; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein kinase B signaling cascade; negative regulation of cell proliferation; sensory perception of sound; establishment of mitotic spindle orientation; negative regulation of Ras protein signal transduction; negative regulation of apoptosis