Typical Testing Data/Standard Curve (for reference only)
Human serine peptidase inhibitor, Kunitz type 1 ELISA Kit | SPINT1 elisa kit
Human Kunitz-type protease inhibitor 1, SPINT1 ELISA Kit
Gene Names
SPINT1; HAI; HAI1; MANSC2
Reactivity
Human
Synonyms
serine peptidase inhibitor; Kunitz type 1; Human Kunitz-type protease inhibitor 1; SPINT1 ELISA Kit; Human Kunitz-type protease inhibitor 1 (SPINT1) ELISA kit; HAI; HAI1; MANSC2; Kunitz-type protease inhibitor 1; hepatocyte growth factor activator inhibitor 1; serine protease inhibitor; SPINT1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human SPINT1. No significant cross-reactivity or interference between human SPINT1 and analogues was observed.
Sequence Length
513
Samples
Serum, plasma, tissue homogenates
Assay Type
Sandwich
Detection Range
0. 156 ng/ml -10 ng/ml.
Sensitivity
The minimum detectable dose of human SPINT1 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SPINT1 elisa kit
Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SPINT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPINT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPINT1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPINT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
58,398 Da
NCBI Official Full Name
kunitz-type protease inhibitor 1 isoform 2
NCBI Official Synonym Full Names
serine peptidase inhibitor, Kunitz type 1
NCBI Official Symbol
SPINT1
NCBI Official Synonym Symbols
HAI; HAI1; MANSC2
NCBI Protein Information
kunitz-type protease inhibitor 1; HAI-1; serine protease inhibitor, Kunitz type 1; hepatocyte growth factor activator inhibitor type 1
UniProt Protein Name
Kunitz-type protease inhibitor 1
Protein Family
UniProt Gene Name
SPINT1
UniProt Synonym Gene Names
HAI1; UNQ223/PRO256; HAI-1
UniProt Entry Name
SPIT1_HUMAN
NCBI Description
The protein encoded by this gene is a member of the Kunitz family of serine protease inhibitors. The protein is a potent inhibitor specific for HGF activator and is thought to be involved in the regulation of the proteolytic activation of HGF in injured tissues. Alternative splicing results in multiple variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
SPINT1: Inhibitor of HGF activator. Also acts as an inhibitor of matriptase (ST14). 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 15q15.1
Cellular Component: extracellular space; membrane; extracellular region
Molecular Function: serine-type endopeptidase inhibitor activity
Biological Process: extracellular matrix organization and biogenesis
Research Articles on SPINT1
Similar Products
Product Notes
The Human SPINT1 spint1 (Catalog #AAA904050) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA904050 ELISA Kit recognizes Human SPINT1. It is sometimes possible for the material contained within the vial of "serine peptidase inhibitor, Kunitz type 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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