Human integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41) ELISA Kit | ITGA2B elisa kit

Human Integrin alpha-IIb, ITGA2B ELISA Kit

Cat. Num. AAA923777

• Gene Names: ITGA2B; GT; GTA; CD41; GP2B; HPA3; CD41B; GPIIb; BDPLT2; BDPLT16; PPP1R93
• Reactivity: Human
• Synonyms: integrin; alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex; antigen CD41); Human Integrin alpha-IIb; ITGA2B ELISA Kit; Human Integrin alpha-IIb (ITGA2B) ELISA kit; CD41; CD41B; GP2B; GPIIb; GTA; HPA3; integrin alpha 2b; platelet fibrinogen receptor; alpha subunit; platelet-specific antigen BAK; ITGA2B elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human ITGA2B. No significant cross-reactivity or interference between human ITGA2B and analogues was observed.
• Sequence Length: 1,039

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 0. 156 ng/ml -10 ng/ml.
• Sensitivity: The minimum detectable dose of human ITGA2B is typically less than 0.039 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ITGA2B elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ITGA2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ITGA2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ITGA2B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ITGA2B bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 88758615
• NCBI GeneID: 3674
• NCBI Accession #: NP_000410.2
• NCBI GenBank Nucleotide #: NM_000419.3
• UniProt Accession #: P08514; O95366; Q14443; Q17R67; B2RCY8
• Molecular Weight: 113,377 Da
• NCBI Official Full Name: integrin alpha-IIb preproprotein
• NCBI Official Synonym Full Names: integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41)
• NCBI Official Symbol: ITGA2B
• NCBI Official Synonym Symbols: GT; GTA; CD41; GP2B; HPA3; CD41B; GPIIb; BDPLT2; BDPLT16; PPP1R93
• NCBI Protein Information: integrin alpha-IIb; GPalpha IIb; platelet-specific antigen BAK; platelet membrane glycoprotein IIb; platelet fibrinogen receptor, alpha subunit; protein phosphatase 1, regulatory subunit 93
• UniProt Protein Name: Integrin alpha-IIb
• Protein Family: Integrin
• UniProt Gene Name: ITGA2B
• UniProt Synonym Gene Names: GP2B; ITGAB; GPIIb
• UniProt Entry Name: ITA2B_HUMAN

NCBI Description

ITGA2B encodes integrin alpha chain 2b. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. Alpha chain 2b undergoes post-translational cleavage to yield disulfide-linked light and heavy chains that join with beta 3 to form a fibronectin receptor expressed in platelets that plays a crucial role in coagulation. Mutations that interfere with this role result in thrombasthenia. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq, Jul 2008]

Uniprot Description

ITGA2B: Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha- IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface. Belongs to the integrin alpha chain family. Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-IIb associates with beta-3. Directly interacts with RNF181. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: platelet alpha granule membrane; cell surface; focal adhesion; integral to plasma membrane; plasma membrane; integrin complex; external side of plasma membrane

Molecular Function: identical protein binding; protein binding; extracellular matrix binding; metal ion binding

Biological Process: integrin-mediated signaling pathway; axon guidance; platelet activation; extracellular matrix organization and biogenesis; platelet degranulation; cell-matrix adhesion; positive regulation of leukocyte migration; cell adhesion; blood coagulation

Disease: Glanzmann Thrombasthenia; Bleeding Disorder, Platelet-type, 16