Typical Testing Data/Standard Curve (for reference only)
(Human SPINT1/HAI-1 PicoKine ELISA Kit standard curve )
Human SPINT1 ELISA Kit | SPINT1 elisa kit
Human SPINT1/HAI-1 PicoKine ELISA Kit
Gene Names
SPINT1; HAI; HAI1; MANSC2
Reactivity
Human
Synonyms
SPINT1; Human SPINT1/HAI-1 PicoKine ELISA Kit; HAI 1; HAI1; HAI-1; MANSC2; SPINT 1; O43278; Kunitz-type protease inhibitor 1; serine peptidase inhibitor; Kunitz type 1; SPINT1 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human SPINT1/HAI-1
Sequence Length
513
Samples
Cell culture supernates, serum and plasma(heparin).
Assay Type
Sandwich
Detection Range
156pg/ml-10000pg/ml
Sensitivity
<15pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).
Typical Testing Data/Standard Curve (for reference only)
(Human SPINT1/HAI-1 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Human SPINT1/HAI-1 PicoKine ELISA Kit standard curve )
Related Product Information for SPINT1 elisa kit
Principle of the Assay: Human SPINT1/HAI-1 ELISA Kit was based on standard sandwich enzyme-linked immunesorbent assay technology. A monoclonal antibody from mouse specific for SPINT1/HAI-1 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: G36-L529) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SPINT1/HAI-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human SPINT1/HAI-1 amount of sample captured in plate.
Background/Introduction: Kunitz-type protease inhibitor 1 is an enzyme that in humans is encoded by the SPINT1 gene. It is a member of the Kunitz family of serine protease inhibitors. The SPINT1 gene encodes an inhibitor of hepatocyte growth factor activator (HGFAC) and matriptase and is involved in degradation of the extracellular matrix. SPINT1 is produced as an active membrane-associated protein and is proteolytically cleaved and secreted. Alternative splicing results in multiple variants encoding different isoforms.
Background/Introduction: Kunitz-type protease inhibitor 1 is an enzyme that in humans is encoded by the SPINT1 gene. It is a member of the Kunitz family of serine protease inhibitors. The SPINT1 gene encodes an inhibitor of hepatocyte growth factor activator (HGFAC) and matriptase and is involved in degradation of the extracellular matrix. SPINT1 is produced as an active membrane-associated protein and is proteolytically cleaved and secreted. Alternative splicing results in multiple variants encoding different isoforms.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
56,885 Da
NCBI Official Full Name
kunitz-type protease inhibitor 1 isoform 2
NCBI Official Synonym Full Names
serine peptidase inhibitor, Kunitz type 1
NCBI Official Symbol
SPINT1
NCBI Official Synonym Symbols
HAI; HAI1; MANSC2
NCBI Protein Information
kunitz-type protease inhibitor 1
UniProt Protein Name
Kunitz-type protease inhibitor 1
Protein Family
UniProt Gene Name
SPINT1
UniProt Synonym Gene Names
HAI1; HAI-1
UniProt Entry Name
SPIT1_HUMAN
NCBI Description
The protein encoded by this gene is a member of the Kunitz family of serine protease inhibitors. The protein is a potent inhibitor specific for HGF activator and is thought to be involved in the regulation of the proteolytic activation of HGF in injured tissues. Alternative splicing results in multiple variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
SPINT1: Inhibitor of HGF activator. Also acts as an inhibitor of matriptase (ST14). 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 15q15.1
Cellular Component: extracellular region; extracellular space; membrane
Research Articles on SPINT1
Similar Products
Product Notes
The Human SPINT1 spint1 (Catalog #AAA178520) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA178520 ELISA Kit recognizes Human SPINT1. It is sometimes possible for the material contained within the vial of "SPINT1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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