Human defensin, alpha 6, Paneth cell-specific ELISA Kit | DEFA6 elisa kit

Human Defensin-6, DEFA6 ELISA Kit

Cat. Num. AAA944129

• Gene Names: DEFA6; DEF6; HD-6
• Reactivity: Human
• Synonyms: defensin; alpha 6; Paneth cell-specific; Human Defensin-6; DEFA6 ELISA Kit; Human Defensin-6 (DEFA6) ELISA kit; DEF6; HD-6; defensin 6; DEFA6 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human DEFA6. No significant cross-reactivity or interference between human DEFA6 and analogues was observed.
• Sequence Length: 100

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 0.156 ng/ml -10 ng/ml.
• Sensitivity: The minimum detectable dose of human DEFA6 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DEFA6 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DEFA6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DEFA6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DEFA6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DEFA6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4503305
• NCBI GeneID: 1671
• NCBI Accession #: NP_001917.1
• NCBI GenBank Nucleotide #: NM_001926.3
• UniProt Accession #: Q01524; Q6EZF9
• Molecular Weight: 10,975 Da
• NCBI Official Full Name: defensin-6 preproprotein
• NCBI Official Synonym Full Names: defensin, alpha 6, Paneth cell-specific
• NCBI Official Symbol: DEFA6
• NCBI Official Synonym Symbols: DEF6; HD-6
• NCBI Protein Information: defensin-6; defensin 6
• UniProt Protein Name: Defensin-6
• Protein Family: Defensin
• UniProt Gene Name: DEFA6
• UniProt Synonym Gene Names: DEF6
• UniProt Entry Name: DEF6_HUMAN

NCBI Description

Defensins are a family of microbicidal and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several alpha defensin genes appear to be clustered on chromosome 8. The protein encoded by this gene, defensin, alpha 6, is highly expressed in the secretory granules of Paneth cells of the small intestine, and likely plays a role in host defense of human bowel. [provided by RefSeq, Jul 2008]

Uniprot Description

defensin, alpha 6: Has very low antimicrobial activity against Gram- negative and Gram-positive bacteria. May protect cells against infection with HIV-1. Belongs to the alpha-defensin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: extracellular space; Golgi lumen; extracellular region

Biological Process: killing of cells of another organism; antibacterial humoral response; innate immune response; defense response to fungus