Human WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2 ELISA Kit | WFIKKN2 elisa kit

Human WAP, kazal, immunoglobulin, kunitz and NTR domain-containing protein 2, WFIKKN2 ELISA Kit

Cat. Num. AAA946744

• Gene Names: WFIKKN2; GASP-1; WFDC20B; hGASP-1; WFIKKNRP
• Reactivity: Human
• Synonyms: WAP; follistatin/kazal; immunoglobulin; kunitz and netrin domain containing 2; Human WAP; kazal; kunitz and NTR domain-containing protein 2; WFIKKN2 ELISA Kit; kunitz and NTR domain-containing protein 2 (WFIKKN2) ELISA kit; WFIKKNRP; FS; Ig; two KU and NTR module related protein; WFIKKN-related protein; WFIKKN2 protein; multivalent protease inhibitor protein; WFIKKN2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human WFIKKN2. No significant cross-reactivity or interference between human WFIKKN2 and analogues was observed.
• Sequence Length: 576

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 0.156 ng/ml-10 ng/ml.
• Sensitivity: The minimum detectable dose of human WFIKKN2 is typically less than 0.039 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for WFIKKN2 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for WFIKKN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WFIKKN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WFIKKN2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WFIKKN2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 28212222
• NCBI GeneID: 124857
• NCBI Accession #: NP_783165.1
• NCBI GenBank Nucleotide #: NM_175575.5
• UniProt Accession #: Q8TEU8; Q6UXZ9
• Molecular Weight: 63,941 Da
• NCBI Official Full Name: WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2
• NCBI Official Synonym Full Names: WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2
• NCBI Official Symbol: WFIKKN2
• NCBI Official Synonym Symbols: GASP-1; WFDC20B; hGASP-1; WFIKKNRP
• NCBI Protein Information: WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2; WFIKKN-related protein; WAP four-disulfide core domain 20B; multivalent protease inhibitor protein; WAP, FS, Ig, two KU and NTR module related protein; growth and differentiation factor-associated serum protein 1; WAP, follistatin, immunoglobulin, kunitz and NTR domain-containing-related protein
• UniProt Protein Name: WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2
• Protein Family: WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein
• UniProt Gene Name: WFIKKN2
• UniProt Synonym Gene Names: GASP1; WFIKKNRP; UNQ9235/PRO31996; GASP-1; hGASP-1
• UniProt Entry Name: WFKN2_HUMAN

NCBI Description

The WFIKKN1 protein contains a WAP domain, follistatin domain, immunoglobulin domain, two tandem Kunitz domains, and an NTR domain. This gene encodes a WFIKKN1-related protein which has the same domain organization as the WFIKKN1 protein. The WAP-type, follistatin type, Kunitz-type, and NTR-type protease inhibitory domains may control the action of multiple types of proteases. [provided by RefSeq, Jul 2008]

Uniprot Description

WFIKKN2: Protease-inhibitor that contains multiple distinct protease inhibitor domains. Probably has serine protease- and metalloprotease-inhibitor activity. Inhibits the biological activity of mature myostatin, but not activin. Belongs to the WFIKKN family.

Protein type: Inhibitor; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17q21.33

Cellular Component: extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; metalloendopeptidase inhibitor activity

Biological Process: transforming growth factor beta receptor signaling pathway; palate development; negative regulation of protein binding; muscle fiber development; skeletal development; negative regulation of DNA binding