Western Blot (WB)
(Western blot analysis of mouse macrophages untreated (lanes 1 & 3) or treated with LPS (1ug/ml) for 18 hrs (lanes 2 & 4). The blots were probed with mouse monoclonal anti-iNOS at 1:500 (lanes 1 & 2) or rabbit polyclonal anti-iNOS at 1:250 (lanes 3 & 4).)
Macrophage + LPS (18 hr) Treated Cell Lysate
Mouse Macrophage + LPS (18 hr) Treated
Applications
Western Blot
Synonyms
Macrophage + LPS (18 hr) Treated; Mouse Macrophage + LPS (18 hr) Treated; Mouse Macrophage + LPS (18 hr); Macro L+; Macrophage + LPS (18 hr) Treated cell lysate
Form/Format
Cell Lysates are supplied at a concentration of 1mg/ml in electrophoresis sample buffer (62.5mM Tris pH6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% beta-mercaptoethanol).
Applicable Applications for Macrophage + LPS (18 hr) Treated cell lysate
Western Blot (WB)
Application Notes
WB: 20ul/lane
Preparation and Storage
Store at -20 degree C. Do not boil or dilute. Stable for 1 year.
Western Blot (WB)
(Western blot analysis of mouse macrophages untreated (lanes 1 & 3) or treated with LPS (1ug/ml) for 18 hrs (lanes 2 & 4). The blots were probed with mouse monoclonal anti-iNOS at 1:500 (lanes 1 & 2) or rabbit polyclonal anti-iNOS at 1:250 (lanes 3 & 4).)
Western Blot (WB)
(Western blot analysis of mouse macrophages untreated (lanes 1 & 3) or treated with LPS (1ug/ml) for 18 hrs (lanes 2 & 4). The blots were probed with mouse monoclonal anti-iNOS at 1:500 (lanes 1 & 2) or rabbit polyclonal anti-iNOS at 1:250 (lanes 3 & 4).)
Related Product Information for Macrophage + LPS (18 hr) Treated cell lysate
Nitric oxide (NO) has a broad range of biological activities and is implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOS) are the enzymes responsible for synthesis of NO and several distinct NOS isoforms are produced from three distinct genes. The inducible form of NOS, iNOS (also designated NOS-II), is Ca2+ independent and is expressed in a broad range of cell types. This form of NOS is induced in macrophages and monocytes after stimulation with cytokines and exposure to microbial products, such as LPS.Confluent cultures of mouse macrophage cells (J774A.1) were either left untreated or treated with LPS (1ug/ml) for 18 hrs at 37 degree C . Cells were lysed in 1% SDS, 1.0mM sodium ortho-vanadate, 1mM sodium fluoride, 10mM Tris (pH7.4) buffer. Protein concentration was determined using the BCA method (Pierce) before diluting to final concentration and buffer. The LPS treated lysate has significant induction of iNOS expression relative to the control cells as demonstrated using anti-iNOS antibodies (NM3981 and NP2131).
References
Kleinert, H. et al. (2003) Biol Chem. 384(10-11):1343.
Xie, Q.W. et al. (1992) Science 256:225.
Xie, Q.W. et al. (1992) Science 256:225.
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Product Notes
The Macrophage + LPS (18 hr) Treated (Catalog #AAA474433) is a Cell Lysate and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Macrophage + LPS (18 hr) Treated can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 20ul/lane. Researchers should empirically determine the suitability of the Macrophage + LPS (18 hr) Treated for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Macrophage + LPS (18 hr) Treated, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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