Mouse Deoxyribonuclease I Like Protein 3 (DNASE1L3) RTU ELISA Kit | DNASE1L3 rtu elisa kit

Mouse Deoxyribonuclease I Like Protein 3 (DNASE1L3) ELISA Kit

Cat. Num. AAA458892

• Gene Names: Dnase1l3; Lsd; Dhp2; DNasegamma
• Reactivity: Mouse
• Synonyms: Deoxyribonuclease I Like Protein 3 (DNASE1L3); Mouse Deoxyribonuclease I Like Protein 3 (DNASE1L3) ELISA Kit; DHP2; DNAS1L3; LSD; Deoxyribonuclease Gamma; Liver and spleen DNase; DNase I homolog protein DHP2; DNASE1L3 rtu elisa kit

• Reactivity: Mouse
• Sequence Length: 310

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.058ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DNASE1L3 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DNASE1L3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to DNASE1L3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DNASE1L3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DNASE1L3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 84490435
• NCBI GeneID: 13421
• NCBI Accession #: NP_031896.3
• NCBI GenBank Nucleotide #: NM_007870.3
• UniProt Accession #: O55070; Q91X38
• Molecular Weight: 35,760 Da
• NCBI Official Full Name: deoxyribonuclease gamma
• NCBI Official Synonym Full Names: deoxyribonuclease 1-like 3
• NCBI Official Symbol: Dnase1l3
• NCBI Official Synonym Symbols: Lsd; Dhp2; DNasegamma
• NCBI Protein Information: deoxyribonuclease gamma
• UniProt Protein Name: Deoxyribonuclease gamma
• Protein Family: Deoxyribonuclease
• UniProt Gene Name: Dnase1l3
• UniProt Synonym Gene Names: DNase gamma; DNase I-like 3; LS-DNase; LSD

Uniprot Description

Has DNA hydrolytic activity. Is capable of both single- and double-stranded DNA cleavage, producing DNA fragments with 3'-OH ends (). Can cleave chromatin to nucleosomal units and cleaves nucleosomal and liposome-coated DNA (PubMed:15796714, PubMed:19154352, PubMed:12095301). Acts in internucleosomal DNA fragmentation (INDF) during apoptosis and necrosis. The role in apoptosis includes myogenic and neuronal differentiation, and BCR-mediated clonal deletion of self-reactive B cells (PubMed:12050166, PubMed:15167901, PubMed:17218958, PubMed:24312463). Is active on chromatin in apoptotic cell-derived membrane-coated microparticles and thus suppresses anti-DNA autoimmunity (PubMed:15796714, PubMed:27293190). Together with DNASE1, plays a key role in degrading neutrophil extracellular traps (NETs) (PubMed:29191910). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (PubMed:29191910). Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation (PubMed:29191910).