Western Blot (WB)
(Western blot of human recombinant SSH1L untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) and rat PC12 cells unstimulated (lanes 5 & 7) or stimulated with calyculin A (lanes 6 & 8). The blots were probed with anti-SSH1L (C-terminal region) (lanes 1, 2, 5, & 6) or anti-SSH1L (Ser-978) (lanes 3, 4, 7, & 8).)
Slingshot-1L Recombinant Protein
Slingshot-1L Recombinant Protein
Applications
Western Blot
Synonyms
Slingshot-1L; Slingshot-1L Recombinant Protein; SlingshotR; Slingshot-1L recombinant protein
Host
Lysate
Form/Format
Slingshot-1L recombinant protein lysate is supplied at a concentration of 1ng/ul in electrophoresis sample buffer (62.5mM Tris pH6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% beta-mercaptoethanol).
Applicable Applications for Slingshot-1L recombinant protein
Western Blot (WB)
Application Notes
WB: 10ul/lane
Preparation and Storage
Store at -20 degree C. Do not boil or dilute. Stable for 1 year.
Western Blot (WB)
(Western blot of human recombinant SSH1L untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) and rat PC12 cells unstimulated (lanes 5 & 7) or stimulated with calyculin A (lanes 6 & 8). The blots were probed with anti-SSH1L (C-terminal region) (lanes 1, 2, 5, & 6) or anti-SSH1L (Ser-978) (lanes 3, 4, 7, & 8).)
Western Blot (WB)
(Western blot of human recombinant SSH1L untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) and rat PC12 cells unstimulated (lanes 5 & 7) or stimulated with calyculin A (lanes 6 & 8). The blots were probed with anti-SSH1L (C-terminal region) (lanes 1, 2, 5, & 6) or anti-SSH1L (Ser-978) (lanes 3, 4, 7, & 8).)
Related Product Information for Slingshot-1L recombinant protein
Members of the ADF/cofilin (AC) family are actin-severing proteins that are regulated through serine phosphorylation and dephosphorylation. Activation of cofilin kinases, LIMK1 or LIMK2, leads to phosphorylation of cofilin at serine 3. This phosphorylation disrupts cofilin binding to actin in vitro and in vivo. Multiple phosphatases, Slingshot, PP1, PP2A, PP2B, and chronophin can dephosphorylate Ser-3 and activate actin binding. In mammals, the Slingshot family includes SSH1L, SSH2L, and SSH3L. SSH1L and SSH2L mRNAs are widely expressed, while SSH3L has high expression in epithelial tissues. SSH1L can associate with F-actin and may be the major phosphatase regulating cofilin activity. Disruption of SSH1L expression using RNA interference impairs directional cell migration. Phosphorylation of SSH1L at Ser-937 and Ser-978 by PKD leads to association with 14-3-3, sequestration of the phosphatase to the cytoplasm, and reduces cell migration.
Human recombinant Slingshot-1L is a full length protein (140kDa) with an N-terminal GST fusion protein. This Slingshot-1L protein is detected by rabbit polyclonal anti-Slingshot-1L .
Human recombinant Slingshot-1L is a full length protein (140kDa) with an N-terminal GST fusion protein. This Slingshot-1L protein is detected by rabbit polyclonal anti-Slingshot-1L .
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Product Notes
The Slingshot-1L (Catalog #AAA474579) is a Recombinant Protein produced from Lysate and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Slingshot-1L can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 10ul/lane. Researchers should empirically determine the suitability of the Slingshot-1L for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Slingshot-1L, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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