Human CD169/SIGLEC-1 ELISA Kit | SIGLEC1 elisa kit

Human CD169/SIGLEC-1 PicoKine ELISA Kit

Cat. Num. AAA177317

• Gene Names: SIGLEC1; SN; CD169; SIGLEC-1; dJ1009E24.1
• Reactivity: Human
• Synonyms: CD169/SIGLEC-1; Human CD169/SIGLEC-1 PicoKine ELISA Kit; Sialoadhesin; sialic acid binding Ig-like lectin 1; sialoadhesin; CD169; CD169 antigen; dJ1009E24.1; DKFZp667F058; FLJ00051; FLJ00055; FLJ00073; FLJ00411; FLJ32150; Sialic acid binding Ig like lectin 1; Sialic acid binding immunoglobulin like lectin 1; Sialic acid-binding Ig-like lectin 1; Siglec 1; Siglec-1; Siglec1; SN; SN_HUMAN; SIGLEC1 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human CD169
• Sequence Length: 1709

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 156pg/ml-10,000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human CD169/SIGLEC-1 PicoKine ELISA Kit standard curve )

Related Product Information for SIGLEC1 elisa kit

Principle of the assay: human CD169 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD169 has been precoated onto 96-well plates. Standards(NSO, S20-Q1641) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD169 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD169 amount of sample captured in plate.
Background: SIGLEC-1, also known as Sialoadhesin or CD169, is a cell adhesion molecule found on the surface of certain cells of the immune system called macrophages. This gene is mapped to 20p13. It belongs to the immunoglobulin superfamily(IgSF). Since sialoadhesin binds sialic acids with its N-terminal IgV-domain, it is also a member of the SIGLEC family. The localization and expression of SIGLEC-1 in humans has altered coincident with the evolutionary loss of Neu5Gc. SIGLEC-1-positive macrophages have a dual physiologic function. They act as innate 'flypaper' by preventing the systemic spread of lymph-borne pathogens and as critical gatekeepers at the lymph-tissue interface that facilitate the recognition of particulate antigens by B cells and initiate humoral immune responses.

NCBI and Uniprot Product Information

• NCBI GI #: 18202745
• NCBI GeneID: 6614
• UniProt Accession #: Q9BZZ2; Q96DL4; Q9GZS5; Q9H1H6; Q9H1H7; Q9H7L7
• Molecular Weight: 181,733 Da
• NCBI Official Full Name: Sialoadhesin
• NCBI Official Synonym Full Names: sialic acid binding Ig-like lectin 1, sialoadhesin
• NCBI Official Symbol: SIGLEC1
• NCBI Official Synonym Symbols: SN; CD169; SIGLEC-1; dJ1009E24.1
• NCBI Protein Information: sialoadhesin; sialic acid-binding Ig-like lectin 1; sialic acid-binding immunoglobulin-like lectin 1
• UniProt Protein Name: Sialoadhesin
• Protein Family: Sialoadhesin
• UniProt Gene Name: SIGLEC1
• UniProt Synonym Gene Names: SN; Siglec-1
• UniProt Entry Name: SN_HUMAN

NCBI Description

This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a lectin-like adhesion molecule that binds glycoconjugate ligands on cell surfaces in a sialic acid-dependent manner. It is a type I transmembrane protein expressed only by a subpopulation of macrophages and is involved in mediating cell-cell interactions. Alternative splicing produces a transcript variant encoding an isoform that is soluble rather than membrane-bound; however, the full-length nature of this variant has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

SIGLEC1: Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid. Binds to SPN/CD43 on T-cells. May play a role in hemopoiesis. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: integral to membrane; plasma membrane; extracellular region

Molecular Function: carbohydrate binding

Biological Process: cell-cell adhesion; cell-matrix adhesion; endocytosis; inflammatory response