Western Blot (WB)
(Western Blot analysis of DDIT4 on Hela (1) and K562 (2) cell lysates using anti-DDIT4 antibody at 1/200 dilution.)
Rabbit DDIT4 Polyclonal Antibody | anti-DDIT4 antibody
DDIT4 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Peptide affinity purified
Synonyms
DDIT4; Polyclonal Antibody; DDIT4 Polyclonal Antibody; REDD-1; REDD1; RTP801; Dig2; DNA damage inducible transcript 4; DNA damage inducible transcript 4 protein; DNA damage-inducible transcript 4 protein; FLJ20500; HIF 1 responsive protein RTP801; HIF 1 responsive RTP801; HIF-1 responsive protein RTP801; Protein regulated in development and DNA damage response 1; anti-DDIT4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
DDIT4 polyclonal antibody detects endogenous levels of DDIT4 protein.
Purity/Purification
Peptide affinity purified
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Applicable Applications for anti-DDIT4 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500
ICC: 1:50-1:100
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
ICC: 1:50-1:100
IHC: 1:50-1:200
FC/FACS: 1:50-1:100
Immunogen
Peptide.
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of DDIT4 on Hela (1) and K562 (2) cell lysates using anti-DDIT4 antibody at 1/200 dilution.)
Western Blot (WB)
(Western Blot analysis of DDIT4 on Hela (1) and K562 (2) cell lysates using anti-DDIT4 antibody at 1/200 dilution.)
Immunocytochemistry (ICC)
(ICC staining DDIT4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining DDIT4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Related Product Information for anti-DDIT4 antibody
REDD-1, also designated DNA-damage-inducible transcript 4, dig2 or RTP801, is thought to function in the regulation of reactive oxygen species (ROS). REDD-1 expression has also been linked to apoptosis, Ab toxicity and the pathogenesis of ischemic diseases. As an HIF-1-responsive gene, REDD-1 exhibits strong hypoxia-dependent upregulation in ischemic cells of neuronal origin. In response to stress due to DNA damage and glucocorticoid treatment, REDD-1 is upregulated at the transcriptional level. REDD-1 negatively regulates the mammalian target of Rapamycin (mTOR), a serine/threonine kinase often referred to as FRAP. It is crucial in the coupling of extra- and intracellular cues to FRAP regulation. The absence of REDD-1 is associated with the development of retinopathy, a major cause of blindness.
NCBI and Uniprot Product Information
Molecular Weight
25kDa (Predicted Band Size)
Protein Family
Similar Products
Product Notes
The DDIT4 (Catalog #AAA3012584) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DDIT4 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DDIT4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500 ICC: 1:50-1:100 IHC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the DDIT4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DDIT4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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