Rabbit KLHL7 Polyclonal Antibody | anti-KLHL7 antibody

KLHL7 Polyclonal Antibody

Cat. Num. AAA3015468

• Gene Names: KLHL7; KLHL6; SBBI26
• Reactivity: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse
• Applications: Immunohistochemistry
• Purity: affinity purified by Protein A
• Synonyms: KLHL7; Polyclonal Antibody; KLHL7 Polyclonal Antibody; Kelch like protein 7; kelch-like 6; kelch-like 7; kelch/BTB.KLHL7_HUMAN; anti-KLHL7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse
• Clonality: Polyclonal
• Specificity: KLHL7 Polyclonal Antibody detects endogenous levels of KLHL7 protein.
• Purity/Purification: affinity purified by Protein A
• Form/Format: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• Applicable Applications for anti-KLHL7 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:100-500
• Immunogen: KLH conjugated synthetic peptide derived from human KLHL7:51-150/856
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLHL7) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C,)

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLHL7) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C)

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLHL7) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C)

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLHL7) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C)

Related Product Information for anti-KLHL7 antibody

This gene encodes a BTB-Kelch-related protein. The encoded protein may be involved in protein degradation. Mutations in this gene have been associated with retinitis pigmentosa 42. [provided by RefSeq, Feb 2010].

NCBI and Uniprot Product Information

• NCBI GI #: 289063419
• NCBI GeneID: 55975
• NCBI Accession #: NP_001165899.1
• NCBI GenBank Nucleotide #: NM_001172428.1
• UniProt Accession #: Q8IXQ5; Q7Z765; Q96MV2; Q9BQF8; Q9UDQ9; A4D144; B7Z5I9; G5E9G3
• Molecular Weight: 65,992 Da
• NCBI Official Full Name: kelch-like protein 7 isoform 3
• NCBI Official Synonym Full Names: kelch-like family member 7
• NCBI Official Symbol: KLHL7
• NCBI Official Synonym Symbols: KLHL6; SBBI26
• NCBI Protein Information: kelch-like protein 7; kelch/BTB; kelch-like 6; kelch-like 7
• UniProt Protein Name: Kelch-like protein 7
• Protein Family: Kelch-like protein
• UniProt Gene Name: KLHL7
• UniProt Entry Name: KLHL7_HUMAN

NCBI Description

This gene encodes a BTB-Kelch-related protein. The encoded protein may be involved in protein degradation. Mutations in this gene have been associated with retinitis pigmentosa 42. [provided by RefSeq, Feb 2010]

Uniprot Description

Function: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex. The BCR(KLHL7) complex acts by mediating ubiquitination and subsequent degradation of substrate proteins. Probably mediates 'Lys-48'-linked ubiquitination. Ref.10

Pathway: Protein modification; protein ubiquitination.

Subunit structure: Homodimer. Component of the BCR(KLHL7) E3 ubiquitin ligase complex, at least composed of CUL3 and KLHL7 and RBX1. Ref.10

Subcellular location: Nucleus Ref.9.

Tissue specificity: Widely expressed, with highest levels in adult and fetal heart, CNS and adult testis. Ref.9

Involvement in disease: Retinitis pigmentosa 42 (RP42) [MIM:612943]: A retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.10 Ref.12 Ref.13 Ref.14

Sequence similarities: Contains 1 BACK (BTB/Kelch associated) domain.Contains 1 BTB (POZ) domain.Contains 6 Kelch repeats.