Typical Testing Data/Standard Curve (for reference only)
Mouse CD169/SIGLEC-1 ELISA Kit | SIGLEC1 elisa kit
Mouse CD169/SIGLEC-1 PicoKine ELISA Kit
Gene Names
Siglec1; Sn; Cd169; Siglec-1
Reactivity
Mouse
Synonyms
CD169/SIGLEC-1; Mouse CD169/SIGLEC-1 PicoKine ELISA Kit; Sialoadhesin; sialic acid binding Ig-like lectin 1; sialoadhesin; CD169; CD169 antigen; dJ1009E24.1; DKFZp667F058; FLJ00051; FLJ00055; FLJ00073; FLJ00411; FLJ32150; Sialic acid binding Ig like lectin 1; Sialic acid binding immunoglobulin like lectin 1; Sialic acid-binding Ig-like lectin 1; Siglec 1; Siglec-1; Siglec1; SN; SN_HUMAN; SIGLEC1 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant Mouse Siglec1
Sequence Length
1695
Samples
Cell culture supernatants, cell lysates, serum and plasma (heparin, edta)
Detection Range
156pg/ml-10,000pg/ml
Sensitivity
<10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SIGLEC1 elisa kit
Principle of the Assay: The Picokine™ Mouse Siglec1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse Siglec1 with a 96-well strip plate that is pre-coated with antibody specific for Siglec1. The detection antibody is a biotinylated antibody specific for Siglec1. The capture antibody is monoclonal antibody from rat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Siglec1 with immunogen: Expression system for standard: NSO; Immunogen sequence: T20-R1639. The kit is analytically validated with ready to use reagents. To measure Mouse Siglec1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or BS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse Siglec1 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Siglec1 in the sample.
Background/Introduction: SIGLEC-1, also known as Sialoadhesin or CD169, is a cell adhesion molecule found on the surface of certain cells of the immune system called macrophages. This gene is mapped to 20p13. It belongs to the immunoglobulin superfamily(IgSF). Since sialoadhesin binds sialic acids with its N-terminal IgV-domain, it is also a member of the SIGLEC family. The localization and expression of SIGLEC-1 in humans has altered coincident with the evolutionary loss of Neu5Gc. SIGLEC-1-positive macrophages have a dual physiologic function. They act as innate 'flypaper' by preventing the systemic spread of lymph-borne pathogens and as critical gatekeepers at the lymph-tissue interface that facilitate the recognition of particulate antigens by B cells and initiate humoral immune responses.
Background/Introduction: SIGLEC-1, also known as Sialoadhesin or CD169, is a cell adhesion molecule found on the surface of certain cells of the immune system called macrophages. This gene is mapped to 20p13. It belongs to the immunoglobulin superfamily(IgSF). Since sialoadhesin binds sialic acids with its N-terminal IgV-domain, it is also a member of the SIGLEC family. The localization and expression of SIGLEC-1 in humans has altered coincident with the evolutionary loss of Neu5Gc. SIGLEC-1-positive macrophages have a dual physiologic function. They act as innate 'flypaper' by preventing the systemic spread of lymph-borne pathogens and as critical gatekeepers at the lymph-tissue interface that facilitate the recognition of particulate antigens by B cells and initiate humoral immune responses.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
172,769 Da
NCBI Official Full Name
Sialoadhesin
NCBI Official Synonym Full Names
sialic acid binding Ig-like lectin 1, sialoadhesin
NCBI Official Symbol
Siglec1
NCBI Official Synonym Symbols
Sn; Cd169; Siglec-1
NCBI Protein Information
sialoadhesin; SER; sheep erythrocyte receptor; sialic acid-binding Ig-like lectin 1
UniProt Protein Name
Sialoadhesin
Protein Family
UniProt Gene Name
Siglec1
UniProt Synonym Gene Names
Sa; Sn; SER; Siglec-1
UniProt Entry Name
SN_MOUSE
Uniprot Description
SIGLEC1: Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid. Binds to SPN/CD43 on T-cells. May play a role in hemopoiesis. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Cellular Component: membrane; extracellular region; integral to membrane; plasma membrane
Molecular Function: protein binding; carbohydrate binding
Biological Process: endocytosis; cell adhesion
Research Articles on SIGLEC1
Similar Products
Product Notes
The Mouse SIGLEC1 siglec1 (Catalog #AAA177150) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177150 ELISA Kit recognizes Mouse SIGLEC1. It is sometimes possible for the material contained within the vial of "CD169/SIGLEC-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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