Sheep tumor necrosis factor (TNF superfamily, member 2) ELISA Kit | TNF elisa kit

Sheep Tumor Necrosis Factor alpha, TNF-alpha ELISA Kit

Cat. Num. AAA701635

• Gene Names: TNF; TNF-alpha
• Reactivity: Sheep
• Synonyms: tumor necrosis factor (TNF superfamily; member 2); Sheep Tumor Necrosis Factor alpha; TNF-alpha ELISA Kit; DADB-70P7.1; DIF; TNF-alpha; TNFA; TNFSF2; APC1 protein; TNF superfamily; member 2; TNF; macrophage-derived; monocyte-derived; cachectin; tumor necrosis factor alpha; TNF elisa kit

• Reactivity: Sheep
• Specificity: This assay has high sensitivity and excellent specificity for detection of bovine TGFbeta2. No significant cross-reactivity or interference between bovine TGFbeta2 and analogues was observed.
• Sequence Length: 234

• Samples: Serum, Plasma, Tissue Homogenates
• Assay Type: Competitive
• Detection Range: 6.25 ng/ml-100 ng/ml
• Sensitivity: 6.25 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TNF elisa kit

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TGFbeta2. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated TGFbeta2. A competitive inhibition reaction is launched between TGFbeta2 (Standards or samples) and Biotin-conjugated TGFbeta2 with the pre-coated antibody specific for TGFbeta2. The more amount of TGFbeta2 in samples, the less antibody bound by Biotin-conjugated TGFbeta2. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of TGFbeta2 in the sample. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 67845989
• NCBI GeneID: 443540
• NCBI Accession #: NP_001020031.1
• NCBI GenBank Nucleotide #: NM_001024860.1
• UniProt Accession #: P23383
• Molecular Weight: 25,536 Da
• NCBI Official Full Name: tumor necrosis factor
• NCBI Official Symbol: TNF
• NCBI Official Synonym Symbols: TNF-alpha
• NCBI Protein Information: tumor necrosis factor; TNF-a; cachectin; tumor necrosis factor alpha; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor ligand superfamily member 2
• UniProt Protein Name: Tumor necrosis factor
• Protein Family: Tumor necrosis factor
• UniProt Gene Name: TNF
• UniProt Synonym Gene Names: TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2
• UniProt Entry Name: TNFA_SHEEP

Uniprot Description

Function: Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.