Rabbit Interferon Stimulating Gene 15 Monoclonal Antibody | anti-IL5 antibody

Interferon Stimulating Gene 15 (ISG15, 15kD ubiquitin-like modifier GIP2, IFI15, Ubiquitin Cross-reactive Protein, UCRP)

Cat. Num. AAA601672

• Gene Names: IL5; EDF; TRF; IL-5
• Reactivity: Equine, Human
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by Protein A and peptide affinity chromatography.
• Synonyms: Interferon Stimulating Gene 15; Monoclonal Antibody; Interferon Stimulating Gene 15 (ISG15; 15kD ubiquitin-like modifier GIP2; IFI15; Ubiquitin Cross-reactive Protein; UCRP); Anti -Interferon Stimulating Gene 15 (ISG15; anti-IL5 antibody

• Host: Rabbit
• Reactivity: Equine, Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 7H28
• Specificity: Recognizes endogenous levels of the uncleaved precursor form of human ISG15 protein at ~15kD. Does not recognize the activated (cleaved) or conjugated forms of ISG15. Does not crossreact with other ubiquitin family members, including ubiquitin, SUMO-1, SUMO-2, SUMO-3 or NEDD8. Species crossreactivity: equine.
• Purity/Purification: Affinity Purified; Purified by Protein A and peptide affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-IL5 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunoprecipitation.; Dilution: Western Blot: 1:1000; Immunoprecipitation: 1:25
• Immunogen: Synthetic peptide corresponding to human Interferon Stimulating Gene 15 (ISG15).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of lysates from HeLa and A549 cells, untreated (-) or treated with IFN-alpha (1000 U/mL) (+) for 24 hours, using MBS601672.)

Related Product Information for anti-IL5 antibody

Interferon Stimulating Gene 15 (ISG15) is a member of the ubiquitin-like protein family and functions in varioMyBioSource pathways from pregnancy to innate immune responses. Expression of ISG15 is stimulated by cellular exposure to type 1 interferons alpha and beta, in addition to infection with viruses such as influenza B. After exposure to type I interferons, both lymphocytes and monocytes, in addition to some fibroblasts and epithelial cells, release ISG15 into culture medium. ISG15 has been shown to function as a cytokine, stimulating interferon gamma secretion by monocytes and macrophages, proliferation of natural killer cells, and chemotactic responses in neutrophils. ISG15 has also been shown to function intracellularly, being covalently conjugated to other proteins by E1 (Ube1L), E2 (UbcH8) and E3 ligases via a multi-step process analogous to ubiquitination. ISG15 is removed from proteins by the ubiquitin processing protease Ubp43. ISG15-protein conjugation (ISGylation) is induced by type 1 interferons, and target proteins include the serine protease inhibitor Serpin 2A, PLC gamma1, ERK1/2, Jak1 and Stat1. Unlike ubiquitination, ISGylation does not target proteins for degradation, rather ISGylation increases Jak1 and Stat1 activity, enhancing the cellular response to interferons.

Product Categories/Family for anti-IL5 antibody

Antibodies; Abs to Interferon

NCBI and Uniprot Product Information

• NCBI GI #: 4504671
• NCBI GeneID: 3567
• NCBI Accession #: NP_000870.1
• NCBI GenBank Nucleotide #: NM_000879.2
• UniProt Accession #: P05113; Q13840
• Molecular Weight: 15,238 Da
• NCBI Official Full Name: interleukin-5
• NCBI Official Synonym Full Names: interleukin 5 (colony-stimulating factor, eosinophil)
• NCBI Official Symbol: IL5
• NCBI Official Synonym Symbols: EDF; TRF; IL-5
• NCBI Protein Information: interleukin-5; T-cell replacing factor; B-cell differentiation factor I; eosinophil differentiation factor
• UniProt Protein Name: Interleukin-5
• Protein Family: Interleukin
• UniProt Gene Name: IL5
• UniProt Synonym Gene Names: IL-5; TRF
• UniProt Entry Name: IL5_HUMAN

NCBI Description

This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq, Jul 2013]

Uniprot Description

IL5: Factor that induces terminal differentiation of late- developing B-cells to immunoglobulin secreting cells. Belongs to the IL-5 family.

Protein type: Secreted, signal peptide; Secreted; Cytokine

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; interleukin-5 receptor binding; growth factor activity; cytokine activity

Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; cytokine and chemokine mediated signaling pathway; positive regulation of transcription, DNA-dependent; positive regulation of B cell proliferation; positive regulation of JAK-STAT cascade; positive regulation of transcription factor activity; immune response; positive regulation of immunoglobulin secretion; inflammatory response; positive regulation of eosinophil differentiation