Mouse anti-Human FosB Monoclonal Antibody | anti-FOSB antibody

FosB

Cat. Num. AAA600155

• Gene Names: FOSB; AP-1; G0S3; GOS3; GOSB; MGC42291; DKFZp686C0818
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: FosB; Monoclonal Antibody; Anti -FosB; anti-FOSB antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 4H90
• Specificity: Recognizes human FosB.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide, before the addition of glycerol to 40%.

• Applicable Applications for anti-FOSB antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 2ug/ml; Band at 46kD.
• Immunogen: Synthetic peptide corresponding to aa2-15 of human FosB.
• Positive Control: HeLa cells
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of FosB in HeLa cell lysate with MBS600155. A protein band of approximate molecular weight of 46 kD is detected.)

Related Product Information for anti-FOSB antibody

The v-Fos oncogene was initially identified as the transforming gene of two independent murine osteosarcoma virus isolates (1,2) and an avian nephroblastoma virus (3). The cellular homolog, c-Fos, encodes a 62kD nuclear phosphoprotein that is rapidly and transiently induced by a variety of agents and functions as a transcriptional regulator for several genes. In contrast to c-Jun proteins, which form homo- and heterodimers, which bind to specific DNA TPA response elements (TREs), c-Fos proteins are only active as heterodimers with members of the Jun gene family (4,5). Murine FosB encodes a nuclear protein of 338 amino acids which has 70% homology with c-Fos, exhibits similar kinetics of expression as c-Fos and forms heterodimers with both c-Jun and Jun B which bind to TRE DNA response elements (6). Functional homologs of c-Fos and FosB include Fra-1 and Fra-2 genes (7).

Product Categories/Family for anti-FOSB antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 49457155
• NCBI GeneID: 2354
• UniProt Accession #: Q6FGK6; A8K9K5; P53539; Q49AD7
• Molecular Weight: 35,995 Da
• NCBI Official Full Name: FOSB
• NCBI Official Synonym Full Names: FBJ murine osteosarcoma viral oncogene homolog B
• NCBI Official Symbol: FOSB
• NCBI Official Synonym Symbols: AP-1; G0S3; GOS3; GOSB; MGC42291; DKFZp686C0818
• NCBI Protein Information: protein fosB; oncogene FOS-B; activator protein 1; G0/G1 switch regulatory protein 3
• UniProt Protein Name: FOSB protein
• Protein Family: Protein
• UniProt Gene Name: FOSB
• UniProt Synonym Gene Names: G0S3
• UniProt Entry Name: Q6FGK6_HUMAN

NCBI Description

The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

FosB: FosB interacts with Jun proteins enhancing their DNA binding activity. Heterodimer. Belongs to the bZIP family. Fos subfamily.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: nucleus

Molecular Function: DNA binding; sequence-specific DNA binding; double-stranded DNA binding; transcription factor activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; response to drug; response to cAMP; response to mechanical stimulus; response to morphine; negative regulation of transcription from RNA polymerase II promoter; female pregnancy; response to progesterone stimulus; cellular response to hormone stimulus; response to corticosterone stimulus