14-3-3 sigma recombinant protein

14-3-3 sigma recombinant protein

Cat. Num. AAA515433

• Gene Names: SFN; YWHAS
• Applications: Western Blot
• Synonyms: 14-3-3 sigma; 14-3-3 sigma recombinant protein; 14-3-3 theta; YWHAQ; 1C5; HS1; 14-3-3; 14-3-3sigma Protein

• Host: E Coli
• Form/Format: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Sequence Length: 1336

• Applicable Applications for 14-3-3 sigma recombinant protein: Western Blot (WB)
• Type: Recombinant Fusion Protein
• Species: Human
• Tag Information: GST tag
• Expression System: E.coli
• Source Note: Recombinant full-length human 14-3-3theta was expressed in E. coli cells
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-PAGE

Related Product Information for 14-3-3 sigma recombinant protein

Recombinant full-length human 14-3-3sigma was expressed in E. coli cells using an N-terminal GST tag.

Scientific Background: 14-3-3σ or stratifin is a protein that is strongly induced by gamma irradiation and other DNA-damaging agents (1). The induction of 14-3-3σ is mediated by a p53 responsive element. Exogenous introduction of 14-3-3σ into cycling cells results in a G2 cell cycle arrest (2). Knockout of 14-3-3σ in cells showed that the cells are unable to maintain cell cycle arrest after DNA damage. The 14-3-3σ -/- cells die as they enter mitosis. This process is associated with a failure of the 14-3-3σ -deficient cells to sequester the proteins that initiate mitosis and prevent them from entering the nucleus. Thus, 14-3-3σ plays an important role in maintaining the G2 checkpoint in cells and preventing mitotic death.

Product Categories/Family for 14-3-3 sigma recombinant protein

Cell Stress & Chaperone Proteins; Signaling Proteins - Cell Stress & Chaperone Proteins

References

1. Chan, T A. et al : 14-3-3-σ is required to prevent mitotic catastrophe after DNA damage. Nature 401:616-620, 1999.
2. Hermeking, H. et al: 14-3-3-σ is a p53-regulated inhibitor of G2/M progression. Molec. Cell 1:3-11, 1997.

NCBI and Uniprot Product Information

• NCBI GI #: 45238846
• NCBI GeneID: 2810
• NCBI Accession #: NP_006133.1
• NCBI GenBank Nucleotide #: NM_006142
• UniProt Accession #: P31947; Q6FH30; Q6FH51; Q96DH0
• Molecular Weight: ~51 kDa
• NCBI Official Full Name: Homo sapiens stratifin (SFN), mRNA
• NCBI Official Synonym Full Names: stratifin
• NCBI Official Symbol: SFN
• NCBI Official Synonym Symbols: YWHAS
• NCBI Protein Information: 14-3-3 protein sigma; 14-3-3 sigma; epithelial cell marker protein 1
• UniProt Protein Name: 14-3-3 protein sigma
• UniProt Gene Name: SFN
• UniProt Synonym Gene Names: HME1
• UniProt Entry Name: 1433S_HUMAN

Uniprot Description

Function: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway

By similarity.p53-regulated inhibitor of G2/M progression.

Subunit structure: Homodimer. Interacts with KRT17 and SAMSN1

By similarity. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. Interacts with GAB2. Interacts with SRPK2. Interacts with COPS6. Interacts with RFWD2; this interaction leads to proteasomal degradation. Ref.8 Ref.10 Ref.13 Ref.16

Subcellular location: Cytoplasm. Nucleus

By similarity. Secreted. Note: May be secreted by a non-classical secretory pathway.

Tissue specificity: Present mainly in tissues enriched in stratified squamous keratinizing epithelium.

Sequence similarities: Belongs to the 14-3-3 family.