14-3-3 theta recombinant protein

14-3-3 theta recombinant protein

Cat. Num. AAA515357

• Gene Names: YWHAQ; 1C5; HS1; 14-3-3
• Applications: Western Blot
• Synonyms: 14-3-3 theta; 14-3-3 theta recombinant protein; 14-3-3 sigma; SFN; stratifin; YWHAS; 14-3-3theta Protein

• Host: E Coli
• Form/Format: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Sequence Length: 2272

• Applicable Applications for 14-3-3 theta recombinant protein: Western Blot (WB)
• Type: Recombinant Fusion Protein
• Species: Human
• Tag Information: GST tag
• Expression System: E.coli
• Source Note: Recombinant full-length human 14-3-3sigma was expressed in E. coli cells
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-PAGE

Related Product Information for 14-3-3 theta recombinant protein

Recombinant full-length human 14-3-3theta was expressed in E. coli cells using an N-terminal GST tag.

Scientific Background: 14-3-3theta¸ (also known as tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide) is a member of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. Through interaction with ASK1, c-jun NH-terminal kinase, and p38 mitogen-activated protein kinase (MAPK), 14-3-3theta¸ plays an important role in controlling apotopsis (1). Induced expression of 14-3-3theta¸ protein has been reported in patients with amyotrophic lateral sclerosis. Additionally, 14-3-3theta¸ has been observed to mediate nucleocytoplasmic shuttling of the N protein (coronavirus nucleocapsid protein) which causes severe acute respiratory syndrome (2).

Product Categories/Family for 14-3-3 theta recombinant protein

Cell Stress & Chaperone Proteins; Signaling Proteins - Cell Stress & Chaperone Proteins

References

1. Lau, J.M. et al: The 14-3-3tau phosphoserine-binding protein is required for cardiomyocyte survival. Mol Cell Biol. 2007, 27(4):1455-66.
2. The severe acute respiratory syndrome coronavirus nucleocapsid protein is phosphorylated and localizes in the cytoplasm by 14-3-3-mediated translocation.J Virol. 2005 Sep;79(17):11476-86.

NCBI and Uniprot Product Information

• NCBI GI #: 514052668
• NCBI GeneID: 10971
• NCBI Accession #: NP_006817.1
• NCBI GenBank Nucleotide #: NM_006826
• UniProt Accession #: P27348; Q567U5; Q5TZU8; Q9UP48; D6W4Z5
• Molecular Weight: ~56 kDa
• NCBI Official Full Name: Homo sapiens tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide (YWHAQ), mRNA
• NCBI Official Synonym Full Names: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, theta polypeptide
• NCBI Official Symbol: YWHAQ
• NCBI Official Synonym Symbols: 1C5; HS1; 14-3-3
• NCBI Protein Information: 14-3-3 protein theta; 14-3-3 protein tau; 14-3-3 protein T-cell
• UniProt Protein Name: 14-3-3 protein theta
• UniProt Gene Name: YWHAQ
• UniProt Entry Name: 1433T_HUMAN

NCBI Description

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negatively regulates the kinase activity of PDPK1. Ref.12

Subunit structure: Homodimer. Interacts with CDK16

By similarity. Interacts with SSH1. Interacts with CDKN1B ('Thr-198' phosphorylated form); the interaction translocates CDKN1B to the cytoplasm. Interacts with GAB2. Interacts with the 'Ser-241' phosphorylated form of PDPK1. Ref.11 Ref.12 Ref.13 Ref.15 Ref.23

Subcellular location: Cytoplasm. Note: In neurons, axonally transported to the nerve terminals.

Tissue specificity: Abundantly expressed in brain, heart and pancreas, and at lower levels in kidney and placenta. Up-regulated in the lumbar spinal cord from patients with sporadic amyotrophic lateral sclerosis (ALS) compared with controls, with highest levels of expression in individuals with predominant lower motor neuron involvement. Ref.10

Post-translational modification: Ser-232 is probably phosphorylated by CK1. Ref.9

Sequence similarities: Belongs to the 14-3-3 family.