Western Blot (WB)
(Western Blot: Sample: Human K562 cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human ALDH1A2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Human Aldehyde Dehydrogenase 1 Family Polyclonal Antibody | anti-ALDH1A2 antibody
Aldehyde Dehydrogenase 1 Family, Member A2 (ALDH1A2) Polyclonal Antibody
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Human K562 cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human ALDH1A2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Human K562 cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human ALDH1A2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Esophagus))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Esophagus))
NCBI and Uniprot Product Information
NCBI Description
This protein belongs to the aldehyde dehydrogenase family of proteins. The product of this gene is an enzyme that catalyzes the synthesis of retinoic acid (RA) from retinaldehyde. Retinoic acid, the active derivative of vitamin A (retinol), is a hormonal signaling molecule that functions in developing and adult tissues. The studies of a similar mouse gene suggest that this enzyme and the cytochrome CYP26A1, concurrently establish local embryonic retinoic acid levels which facilitate posterior organ development and prevent spina bifida. Four transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, May 2011]
Uniprot Description
ALDH1A2: Recognizes as substrates free retinal and cellular retinol-binding protein-bound retinal. Does metabolize octanal and decanal but does not metabolize citral, benzaldehyde, acetaldehyde and propanal efficiently. Belongs to the aldehyde dehydrogenase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cofactor and Vitamin Metabolism - retinol; EC 1.2.1.36; Oxidoreductase
Chromosomal Location of Human Ortholog: 15q21.3
Cellular Component: cytoplasm; cytosol; perinuclear region of cytoplasm
Molecular Function: 3-chloroallyl aldehyde dehydrogenase activity; aldehyde dehydrogenase (NAD) activity; retinal binding; retinal dehydrogenase activity
Biological Process: 9-cis-retinoic acid biosynthetic process; anterior/posterior pattern specification; blood vessel development; cardiac muscle tissue development; determination of bilateral symmetry; embryonic camera-type eye development; embryonic forelimb morphogenesis; embryonic gut development; heart morphogenesis; hindbrain development; kidney development; liver development; lung development; midgut development; morphogenesis of embryonic epithelium; negative regulation of cell proliferation; neural crest cell development; neural tube development; neuron differentiation; pancreas development; pituitary gland development; positive regulation of apoptosis; positive regulation of cell proliferation; proximal/distal pattern formation; regulation of endothelial cell proliferation; response to cytokine; response to estradiol; response to vitamin A; retinal metabolic process; retinoic acid metabolic process; retinoic acid receptor signaling pathway; retinol metabolic process; vitamin A metabolic process
Research Articles on ALDH1A2
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Product Notes
The ALDH1A2 aldh1a2 (Catalog #AAA2032120) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Aldehyde Dehydrogenase 1 Family, Member A2 (ALDH1A2) Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Aldehyde Dehydrogenase 1 Family can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the ALDH1A2 aldh1a2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aldehyde Dehydrogenase 1 Family, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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