Sequence
(There are 10 selenocysteine (U) in the original sequence. The amino acids at multiple sites are ‘U’ in UniProt (yellow highlighted; U59S, U300S, U318S, U330S, U345S, U352S, U367S, U369S, U376S, U378S), reference:: https://www.uniprot.org/uniprot/P49908 ). The ‘U’ at each yellow highlighted site has been mutated to ‘S’ to manufacture the MBS1065524 Recombinant Human Selenoprotein P. 'U' selenocysteine is encoded by UGA and it is normally used as a stop codon in prokaryotic cells, which will lead to early termination of translation, hence the lab mutates this selenocysteine for protein manufacturing. In terms of the formation of selenocysteine, selenocysteine is actually a derivative of serine. Selenocysteine and cysteine as well as serine are all highly similar. This selenocysteine can be regarded as either the 'S' of cysteine replaced by Se, or the 'O' of serine replaced by Se. The selenocysteine can mutated to be either serine or cysteine.Generally, the lab mutates 'U' to serine for protein expression because mutating the 'U' to cysteine may result in the formation of additional disulfide bonds, potentially affecting the expression and structure of the protein.)
Selenoprotein P (SEPP1) Recombinant Protein | SEPP1 recombinant protein
Recombinant Human Selenoprotein P (SEPP1) (U59S, U300S, U318S, U330S, U345S, U352S, U367S, U369S, U376S, U378S)
Sequence
(There are 10 selenocysteine (U) in the original sequence. The amino acids at multiple sites are ‘U’ in UniProt (yellow highlighted; U59S, U300S, U318S, U330S, U345S, U352S, U367S, U369S, U376S, U378S), reference:: https://www.uniprot.org/uniprot/P49908 ). The ‘U’ at each yellow highlighted site has been mutated to ‘S’ to manufacture the MBS1065524 Recombinant Human Selenoprotein P. 'U' selenocysteine is encoded by UGA and it is normally used as a stop codon in prokaryotic cells, which will lead to early termination of translation, hence the lab mutates this selenocysteine for protein manufacturing. In terms of the formation of selenocysteine, selenocysteine is actually a derivative of serine. Selenocysteine and cysteine as well as serine are all highly similar. This selenocysteine can be regarded as either the 'S' of cysteine replaced by Se, or the 'O' of serine replaced by Se. The selenocysteine can mutated to be either serine or cysteine.Generally, the lab mutates 'U' to serine for protein expression because mutating the 'U' to cysteine may result in the formation of additional disulfide bonds, potentially affecting the expression and structure of the protein.)
Sequence
(There are 10 selenocysteine (U) in the original sequence. The amino acids at multiple sites are ‘U’ in UniProt (yellow highlighted; U59S, U300S, U318S, U330S, U345S, U352S, U367S, U369S, U376S, U378S), reference:: https://www.uniprot.org/uniprot/P49908 ). The ‘U’ at each yellow highlighted site has been mutated to ‘S’ to manufacture the MBS1065524 Recombinant Human Selenoprotein P. 'U' selenocysteine is encoded by UGA and it is normally used as a stop codon in prokaryotic cells, which will lead to early termination of translation, hence the lab mutates this selenocysteine for protein manufacturing. In terms of the formation of selenocysteine, selenocysteine is actually a derivative of serine. Selenocysteine and cysteine as well as serine are all highly similar. This selenocysteine can be regarded as either the 'S' of cysteine replaced by Se, or the 'O' of serine replaced by Se. The selenocysteine can mutated to be either serine or cysteine.Generally, the lab mutates 'U' to serine for protein expression because mutating the 'U' to cysteine may result in the formation of additional disulfide bonds, potentially affecting the expression and structure of the protein.)
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a selenoprotein containing multiple selenocysteine (Sec) residues, which are encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This selenoprotein is an extracellular glycoprotein, and is unusual in that it contains 10 Sec residues per polypeptide. It is a heparin-binding protein that appears to be associated with endothelial cells, and has been implicated to function as an antioxidant in the extracellular space. Several transcript variants, encoding either the same or different isoform, have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
SEPP1: Might be responsible for some of the extracellular antioxidant defense properties of selenium or might be involved in the transport of selenium. May supply selenium to tissues such as brain and testis. Belongs to the selenoprotein P family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 5q31
Cellular Component: extracellular region; extracellular space
Molecular Function: selenium binding
Biological Process: brain development; growth; locomotory behavior; post-embryonic development; response to oxidative stress; selenium metabolic process; sexual reproduction
Research Articles on SEPP1
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Product Notes
The SEPP1 sepp1 (Catalog #AAA1065524) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 20-381aa (U59S,U300S,U318S,U330S,U345S,U352S,U367S,U369S,U376S,U378S) ;Full Length of Mature Protein. The amino acid sequence is listed below: ESQDQSSLCK QPPAWSIRDQ DPMLNSNGSV TVVALLQASS YLCILQASKL EDLRVKLKKE GYSNISYIVV NHQGISSRLK YTHLKNKVSE HIPVYQQEEN QTDVWTLLNG SKDDFLIYDR CGRLVYHLGL PFSFLTFPYV EEAIKIAYCE KKCGNCSLTT LKDEDFCKRV SLATVDKTVE TPSPHYHHEH HHNHGHQHLG SSELSENQQP GAPNAPTHPA PPGLHHHHKH KGQHRQGHPE NRDMPASEDL QDLQKKLCRK RCINQLLCKL PTDSELAPRS SCCHCRHLIF EKTGSAITSQ CKENLPSLCS SQGLRAEENI TESCQSRLPP AASQISQQLI PTEASASSRS KNQAKKSESP SN. It is sometimes possible for the material contained within the vial of "Selenoprotein P (SEPP1), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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