Selenoprotein P (SEPP1) Recombinant Protein | SEPP1 recombinant protein

Recombinant Bovine Selenoprotein P (SEPP1)

Cat. Num. AAA964014

• Gene Names: SEPP1; SELP
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Selenoprotein P (SEPP1); Recombinant Bovine Selenoprotein P (SEPP1); Selenoprotein P; SeP; Selenoprotein P-like protein; SEPP1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 20-402aa; Full Length of Mature Protein
• Sequence: ESQGQSSYCKQPPPWSIKDQDPMLNSYGSVTVVALLQASSYLCILQASRLEDLRVKLEKEGYSNISYVVVNHQGISSRLKYVHLKNKVSEHIPVYQQEENQPDVWTLLNGNKDDFLIYDRCGRLVYHLGLPYSFLTFTYVEDSIKTVYCEDKCGNCSLKALEDEDVCKNVFLATKEKTAEASQRHHHPHPHSHPHPHPHPHPHPHPHPHHGHQLHENAHLSESPKPDTPDTPENPPPSGLHHHHHRHKGPQRQGHSDNCDTPVGSESLQPSLPQKKLSRKRCINQLLSQFPKDSESALSSCCCHCRHLVFEKTGSAITSQCTEKLPSLCSSQGLLAEENVIESSQSRLPPAASQAAGQQLNPTEASTKSSSKNKAKMSKSPSN (The original sequence contains 12 'U's (P49907: U59S, U297S, U307S, U338S, U350S, U363S, U365S, U372S, U388S, U390S, U397S, U399S) that have already been replaced with 'S' (serine) in the sequence. The 'U' selenocysteine is encoded by UGA and is typically used as a stop codon in prokaryotic cells.)

• Sequence Positions: 20-402. Full Length of Mature Protein. The original sequence contains 12 'U's (P49907) that have already been replaced with 'S' (serine) in the sequence. The 'U' selenocysteine is encoded by UGA and is typically used as a stop codon in prokaryotic cells, which will lead to early termination of translation. Hence, 'U's have mutated with 'S' for protein manufacture.
• Species: Bos taurus (Bovine)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Sequence Information

(The original sequence contains 12 'U's (yellow highlights) UniProt (reference:: (P49907) that have been mutated to 'S' in the sequence for manufacturing the MBS964014 Bovine Selenoprotein P recombinant protein. The 'U' selenocysteine is encoded by UGA and is typically used as a stop codon in prokaryotic cells, which will lead to early termination of translation. Hence, the lab mutates the selenocysteine which is a derivative of serine. Selenocysteine and cysteine as well as serine are highly similar. The selenocysteine can be regarded as either the 'S' atom of cysteine replaced by Se (selenium atom) , or the 'O' atom of serine replaced by Se (selenium atom). Selenocysteine can mutated to either serine or cysteine. The lab has mutated selenocysteine 'U' to serine for protein expression because mutating 'U' to cysteine can lead to the formation of additional disulfide bonds, which can affect the expression and structure of the recombinant protein)

SDS-PAGE

Related Product Information for SEPP1 recombinant protein

Constitutes a major selenium pool in the brain and may play an important role in developing and/or modulating the morphology of neurons and/or glial cells.

NCBI and Uniprot Product Information

• NCBI GI #: 156631001
• NCBI GeneID: 282066
• NCBI Accession #: NP_776884.2
• NCBI GenBank Nucleotide #: NM_174459.3
• UniProt Accession #: P49907; O19003; Q9N2H6
• Molecular Weight: 70.1 kDa
• NCBI Official Full Name: selenoprotein P
• NCBI Official Symbol: SEPP1
• NCBI Official Synonym Symbols: SELP
• NCBI Protein Information: selenoprotein P; seP; selenoprotein P-like protein
• UniProt Protein Name: Selenoprotein P
• Protein Family: Selenoprotein
• UniProt Gene Name: SEPP1
• UniProt Synonym Gene Names: SELP; SeP
• UniProt Entry Name: SEPP1_BOVIN

NCBI Description

This gene encodes a selenoprotein that is predominantly expressed in the liver and secreted into the plasma. This selenoprotein is unique in that it contains multiple selenocysteine (Sec) residues per polypeptide (12 in cow), and accounts for most of the selenium in plasma. It has been implicated as an extracellular antioxidant, and in the transport of selenium to extra-hepatic tissues via apolipoprotein E receptor-2 (apoER2). Mice lacking this gene exhibit neurological dysfunction, suggesting its importance in normal brain function. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. The mRNA for this selenoprotein contains two SECIS elements. [provided by RefSeq, Feb 2017]

Uniprot Description

Constitutes a major selenium pool in the brain and may play an important role in developing and/or modulating the morphology of neurons and/or glial cells.