Related Product Information for SEPP1 elisa kit
Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat SEPP1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat SEPP1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat SEPP1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat SEPP1 can be calculated.
NCBI Description
This gene encodes a selenoprotein containing multiple selenocysteine (Sec) residues, which are encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This selenoprotein is an extracellular glycoprotein, and is unusual in that it contains 10 Sec residues per polypeptide. It is a heparin-binding protein that appears to be associated with endothelial cells, and has been implicated to function as an antioxidant in the extracellular space. Several transcript variants, encoding either the same or different isoform, have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
SEPP1: Might be responsible for some of the extracellular antioxidant defense properties of selenium or might be involved in the transport of selenium. May supply selenium to tissues such as brain and testis. Belongs to the selenoprotein P family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 5q31
Cellular Component: extracellular region; extracellular space
Molecular Function: selenium binding
Biological Process: brain development; growth; locomotory behavior; post-embryonic development; response to oxidative stress; selenium metabolic process; sexual reproduction
Product Notes
The Rat SEPP1 sepp1 (Catalog #
AAA765295) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA765295 ELISA Kit recognizes Rat SEPP1.
It is sometimes possible for the material contained within the vial of
"Selenoprotein P, ELISA Kit" to become dispersed throughout the inside of
the vial, particularly around the seal of said vial, during shipment and storage. We always
suggest centrifuging these vials
to consolidate all of the liquid away from the lid and to the bottom of the vial prior to
opening. Please be advised that
certain products may require dry ice for shipping and that, if this is the case, an
additional dry ice fee may also be
required.
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