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266 results for "autophagic-related-protein" - showing 1-50

Western Blot (WB) (Anti-MAP1LC3A Antibody at 1:5000 dilution + recombinant protein Lysates/proteins at 20 ug per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.)

LC3, Monoclonal Antibody (Cat# AAA9200215)

Full Name
LC3 Antibody (APG8)
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human, mouse, rat
Applications
WB, EIA
Purity
This antibody is purified through a protein G column, followed by dialysis against PBS.
Pricing
$190/0.05 mL | $435/0.2 mL | $1,705/5x0.2 mL
Immunofluorescence (IF) (Fluorescent image of U251 cells stained with cleaved LC3B antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9205943 cleaved LC3B primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)

Cleaved LC3B, Polyclonal Antibody (Cat# AAA9205943)

Full Name
Cleaved LC3B Antibody
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, Mouse (Predicted Reactivity: Bovine)
Applications
IF, EIA, ICC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB) (Western blot analysis of lysates from A431 cell line, untreated or treated with chloroquine, 100ng/ml, using Cleaved-APG8a (MAP1LC3A) Antibody (upper) or Beta-actin (lower).)

Cleaved LC3A, Polyclonal Antibody (Cat# AAA9202198)

Full Name
Cleaved LC3A Antibody
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human, mouse (Predicted Reactivity: Rat, Bovine)
Applications
WB, EIA, IHC, IF, ICC
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (Western blot analysis of lysate from SH-SY5Y cell line, using APG5L Antibody (D3). AP1812a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)

ATG5, Polyclonal Antibody (Cat# AAA9206419)

Full Name
ATG5 Antibody (N-term)
Gene Names
ATG5; ASP; APG5; APG5L; hAPG5; APG5-LIKE
Reactivity
Human;
(Predicted Reactivity: Zebrafish, Bovine, Mouse, Pig, Rat, Rabbit )
Applications
WB, EIA, IHC-P, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB) (Western blot analysis of lc3A/Bexpression in HeLa whole cell lysate.The lane on the left is treated with the antigen-specific peptide.)

lc3A/B, Polyclonal Antibody (Cat# AAA9601573)

Full Name
lc3A/B Antibody
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Brain stained with ATG5 Mouse Monoclonal Antibody (ATG5/2101).)

ATG5, Monoclonal Antibody (Cat# AAA4380520)

Full Name
ATG5 (Autophagy Marker)
Gene Names
ATG5; ASP; APG5; APG5L; hAPG5; SCAR25; APG5-LIKE
Reactivity
Human. Others not known.
Applications
EIA, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunofluorescence (IF) (Immunofluorescent analysis of Hela cells, using Ubiquilin1 Antibody (Center). MBS9204638 was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).)

Ubiquilin1, Polyclonal Antibody (Cat# AAA9204638)

Full Name
Ubiquilin1 Antibody (Center)
Gene Names
UBQLN1; DA41; DSK2; UBQN; XDRP1; PLIC-1
Reactivity
Reactivity: Human, Mouse
Predicted: Rat
Applications
EIA, IHC-P, WB, IF
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB) (Immunoblots of phosphorylated LC3 (phospho-LC3) in CHO cell culture. LC3 and LC3 S12A mutant vectors were transfected into CHO cells. The cell lysates were separated with SDS-PAGE and blotted with anti-phospho-LC3 S12 antibody. LC3 = microtubule-associated protein light chain-3; S12A = replacement of the amino acid position 12 serine of LC3 with alanine. WT = wildtype LC3-transfected cell lysates; S12A = LC3 S12A mutant-transfected cell lysates; Empty vector = vector with no LC3 gene. Molecular size: LC3-I = 16kDa, and LC3-II = 14 kDa)

Phospho-LC3C (S12), Polyclonal Antibody (Cat# AAA9204824)

Full Name
Phospho-LC3C (S12) Antibody
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human
Predicted Reactivity: Zebrafish, Bovine, Mouse, Rat
Applications
WB, DB, EIA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

LC3, Polyclonal Antibody (Cat# AAA9210748)

Full Name
LC3 Antibody (APG8A/B)
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human
Predicted: Zebrafish, Bovine,Mouse, Rat
Applications
WB, EIA, IHC
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB) (Western blot analysis of Hela cell lysate, using MAP1LC3A Antibody. The lane on the left is treated with the antigen-specific peptide.)

MAP1LC3A, Polyclonal Antibody (Cat# AAA9608058)

Full Name
MAP1LC3A Antibody
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (Fluorescent image of U251 cells stained with APG8a/b (MAP1LC3A/B) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9214893 APG8a/b (MAP1LC3A/B) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). APG8a/b (MAP1LC3A/B) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)

APG8a/b (MAP1LC3A/B), Polyclonal Antibody (Cat# AAA9214893)

Full Name
APG8a/b (MAP1LC3A/B) Antibody
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, mouse (Predicted Reactivity: Bovine)
Applications
IF, EIA, WB, IHC
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB) (Western blot analysis of anti-LC3 (APG8a) Pab in rat brain lysate. Both lipidated (arrow, II) and non-lipidated APG8a (arrow, I) were detected in membrane fraction (P) but only non-lipidated LC3 was detected in soluble fraction (S).)

LC3, Polyclonal Antibody (Cat# AAA9201218)

Full Name
LC3 Antibody (APG8A) (N-term)
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human, mouse, rat
Applications
WB, EIA, IHC, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Brain stained with ATG5 Mouse Monoclonal Antibody (ATG5/2492).)

ATG5, Monoclonal Antibody (Cat# AAA4381422)

Full Name
ATG5 (Autophagy Marker)
Gene Names
ATG5; ASP; APG5; APG5L; hAPG5; SCAR25; APG5-LIKE
Reactivity
Human
Applications
EIA, WB, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Brain stained with ATG5 Mouse Monoclonal Antibody (ATG5/2553).)

ATG5, Monoclonal Antibody (Cat# AAA4381423)

Full Name
ATG5 (Autophagy Marker)
Gene Names
ATG5; ASP; APG5; APG5L; hAPG5; SCAR25; APG5-LIKE
Reactivity
Human
Applications
EIA, WB, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunofluorescence (IF) (Fluorescent image of U251 cells stained with ATG9A (C-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9208371 ATG9A (C-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG9A immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)

ATG9A, Polyclonal Antibody (Cat# AAA9208371)

Full Name
ATG9A Antibody (C-term)
Gene Names
ATG9A; mATG9; APG9L1; MGD3208
Reactivity
Human
Applications
IF, EIA, WB, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunofluorescence (IF) (Fluorescent image of U251 cells stained with MBS9203938 LC3 (APG8A) (P45) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9203938 LC3 (APG8A) (P45) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)

LC3, Polyclonal Antibody (Cat# AAA9203938)

Full Name
LC3 Antibody (APG8A) (P45)
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Applications
EIA, IHC, WB, IF
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunofluorescence (IF) (Fluorescent image of U251 cells stained with ATG12 (N-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9213526 ATG12 (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG12 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)

ATG12, Polyclonal Antibody (Cat# AAA9213526)

Full Name
ATG12 Antibody (N-term)
Gene Names
ATG12; APG12; FBR93; APG12L; HAPG12
Reactivity
Human
Applications
IF, EIA, WB, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunofluorescence (IF) (Fluorescent image of U251 cells stained with ATG4D (C-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9211764 ATG4D (C-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG4D immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)

ATG4D, Polyclonal Antibody (Cat# AAA9211764)

Full Name
ATG4D Antibody (C-term)
Gene Names
ATG4D; APG4D; AUTL4; APG4-D
Reactivity
Human
Applications
IF, EIA, WB, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunofluorescence (IF) (Fluorescent image of U251 cells stained with ATG4D (N-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9210910 ATG4D (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 uM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Pictures were taken on a Biorevo microscope (BZ-900, Keyence). ATG4D (N-term) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)

ATG4D, Polyclonal Antibody (Cat# AAA9210910)

Full Name
ATG4D Antibody (N-term)
Gene Names
ATG4D; APG4D; AUTL4; APG4-D
Reactivity
Mouse
Applications
IF, EIA, WB, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Uterus stained with ATG5 Mouse Monoclonal Antibody (rATG5/2553).)

ATG5, Antibody (Cat# AAA4381424)

Full Name
ATG5 (Autophagy Marker)
Gene Names
ATG5; ASP; APG5; APG5L; hAPG5; SCAR25; APG5-LIKE
Reactivity
Human
Applications
IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB) (Western blot analysis of APG5 (arrow) using rabbit polyclonal APG5 Antibody. 293T cell lysates either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG5 gene.)

ATG5, Polyclonal Antibody (Cat# AAA9203213)

Full Name
ATG5 Antibody
Gene Names
ATG5; ASP; APG5; APG5L; hAPG5; APG5-LIKE
Reactivity
Human
Applications
WB, EIA, IHC, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB) (Western blot analysis of hAPG12L-T23 in MCF-7 cell line lysates (35ug/lane). APG12L (arrow) was detected using the purified Pab.)

ATG12, Polyclonal Antibody (Cat# AAA9203753)

Full Name
ATG12 Antibody (N-term)
Gene Names
ATG12; APG12; FBR93; APG12L; HAPG12
Reactivity
Human, mouse
Applications
WB, EIA, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunofluorescence (IF) (Fluorescent image of U251 cells stained with ATG4B (C-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9206388 ATG4B (C-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG4B immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)

ATG4B, Polyclonal Antibody (Cat# AAA9206388)

Full Name
ATG4B Antibody (C-term)
Gene Names
ATG4B; APG4B; AUTL1
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
IF, EIA, WB, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunofluorescence (IF) (Fluorescent image of U251 cells stained with ATG7 antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9213191 ATG7 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG7 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000197548).)

ATG7, Polyclonal Antibody (Cat# AAA9213191)

Full Name
ATG7 Antibody
Gene Names
ATG7; GSA7; APG7L; APG7-LIKE
Reactivity
Human, mouse (Predicted Reactivity: Rat)
Applications
IF, EIA, WB, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
SDS-Page (SDS-PAGE Analysis Purified ATG5 Recombinant Rabbit Monoclonal Antibody (AGT5/3220R). Confirmation of Purity and Integrity of Antibody.)

ATG5, Monoclonal Antibody (Cat# AAA4381542)

Full Name
ATG5 (Autophagy Marker)
Gene Names
ATG5; ASP; APG5; APG5L; hAPG5; SCAR25; APG5-LIKE
Reactivity
Human
Applications
EIA
Purity
Purified Ab with BSA and Azide at 200ug/ml or Purified Ab with BSA and Azide at 200ug/ml or Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunofluorescence (IF) (Fluorescent image of U251 cells stained with LC3 (APG8A) (D106) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9201691 LC3 (APG8A) (D106) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)

LC3, Polyclonal Antibody (Cat# AAA9201691)

Full Name
LC3 Antibody (APG8A) (D106)
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human
Applications
IF, EIA, WB
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunofluorescence (IF) (Fluorescent image of U251 cells stained with ATG16L antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9206351 ATG16L primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG16L immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000085978).)

ATG16L, Polyclonal Antibody (Cat# AAA9206351)

Full Name
ATG16L Antibody
Gene Names
ATG16L1; IBD10; WDR30; APG16L; ATG16A; ATG16L
Reactivity
Human
Applications
IF, EIA, WB
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB) (Western blot analysis of hAPG12L-T23 in MCF-7 cell line lysates (35ug/lane). APG12L (arrow) was detected using the purified Pab.)

ATG12, Polyclonal Antibody (Cat# AAA648113)

Full Name
ATG12, NT (APG12, APG12L, HAPG12, APG12 Autophagy 12-like, APG12-like, Apg12 (Autophagy, yeast) Homolog, Autophagy-related Protein 12)
Gene Names
ATG12; APG12; FBR93; APG12L; HAPG12
Reactivity
Human, Mouse
Applications
EL/EIA, WB, IF
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Pricing
$675/0.2 mL | $3,020/5x0.2 mL
Immunofluorescence (IF) (Fluorescent image of U251 cells stained with APG5 antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1812c APG5 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). ATG5 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)

APG5, Polyclonal Antibody (Cat# AAA641681)

Full Name
APG5 (ATG5, APG5L, ASP, Autophagy protein 5, APG5-like, Apoptosis-specific protein)
Gene Names
ATG5; ASP; APG5; APG5L; hAPG5; APG5-LIKE
Reactivity
Human
Applications
EL/EIA, WB, IHC
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Pricing
$675/0.2 mL | $3,020/5x0.2 mL

Autophagy-related protein 9 (ATG9), Recombinant Protein (Cat# AAA1085060)

Full Name
Recombinant Cryptococcus neoformans var. neoformans serotype D Autophagy-related protein 9 (ATG9)
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$3,185/1 mg (E-Coli) | $3,675/1 mg (Yeast)
Immunofluorescence (IF) (Fluorescent image of U251 cells stained with APG8a/b (MAP1LC3A/B) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP10648a APG8a/b (MAP1LC3A/B) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). APG8a/b (MAP1LC3A/B) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)

MAPG8a/b, Polyclonal Antibody (Cat# AAA644349)

Full Name
MAPG8a/b (MAP1LC3A/B, MAP1LC3B, MAP1ALC3, Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, Microtubule-a
Reactivity
Human, Mouse
Applications
EL/EIA, WB, IHC
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Pricing
$665/0.2 mL | $2,975/5x0.2 mL
Immunocytochemistry (ICC)/Immunofluorescence (IF) (IC/IF analysis: U251 cells were treated with Chloroquine (50uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with antibody MBS642917 (1:200, 2 h at room temperature). Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h) as secondary antibody. Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells)

MAP1LC3A, Monoclonal Antibody (Cat# AAA642917)

Full Name
MAP1LC3A (LC3, APG8, Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated protein 1 l
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human, Mouse, Rat
Applications
EL/EIA, WB, IHC, IF
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Pricing
$695/0.2 mL | $3,110/5x0.2 mL
Western Blot (WB) (Western blot under reducing conditions on Olfactory cell line (Odora) lysate using MBS616452 at a dilution of 1:100.)

MAP1LC3B, Polyclonal Antibody (Cat# AAA616452)

Full Name
MAP1LC3B (LC3B, Autophagy Related Protein LC3B, Autophagy Related Ubiquitin-like Modifier LC3B, MAP1 Light Chain 3-like Protein 2, Microtubule Associated Proteins 1A/1B Light Chain 3B, MAP1A/1B Light Chain 3B, MAP1A/MAP1B LC3B, Microtubule Associated Prot
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, Rat
Applications
EL/EIA, WB, IHC, IF
Purity
Serum
Serum
Pricing
$640/0.1 mL | $2,875/5x0.1 mL
Immunofluorescence (IF) (Fluorescent image of U251 cells stained with ATG12 (N-term) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1816a ATG12 (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). ATG12 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)

ATG12, Polyclonal Antibody (Cat# AAA6003354)

Full Name
ATG12, NT (ATG12, APG12, APG12L, Ubiquitin-like protein ATG12, Autophagy-related protein 12)
Gene Names
ATG12; APG12; FBR93; APG12L; HAPG12
Reactivity
Human
Applications
EL/EIA, WB, IHC
Purity
Purified
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$675/0.2 mL | $3,020/5x0.2 mL
Immunofluorescence (IF) (Fluorescent image of U251 cells stained with ULK1 (phospho S556) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP3804a ULK1 (phospho S556) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). ULK1 (phospho S556) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)

ULK1, Polyclonal Antibody (Cat# AAA6006914)

Full Name
ULK1, phosphorylated (S556) (ULK1, KIAA0722, Serine/threonine-protein kinase ULK1, Autophagy-related protein 1 homolog, Unc-51-like kinase 1)
Gene Names
Ulk1; Unc51.1; AU041434; mKIAA0722
Reactivity
Human
Applications
EL/EIA, GS/EMSA
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Pricing
$675/0.2 mL | $3,020/5x0.2 mL
Immunofluorescence (IF) (Fluorescent image of U251 cells stained with ULK1 (phospho S556) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9208058 ULK1 (phospho S556) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ULK1 (phospho S556) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)

Phospho-ULK1 (S556), Polyclonal Antibody (Cat# AAA9208058)

Full Name
Phospho-ULK1 (S556) Antibody
Gene Names
ULK1; ATG1; ATG1A; UNC51; hATG1; Unc51.1
Reactivity
Human
Applications
IF, DB, EIA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunofluorescence (IF) (Fluorescent image of U251 cells stained with cleaved LC3B antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1806a cleaved LC3B primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)

MAP1ALC3, cleaved, Polyclonal Antibody (Cat# AAA6010760)

Full Name
MAP1ALC3, cleaved (LC3B, MAP1LC3B, Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, Microtubule-associat
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, Mouse
Applications
EL/EIA, ICC, IF
Purity
Purified
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$675/0.2 mL | $3,020/5x0.2 mL
Immunofluorescence (IF) (Fluorescent image of U251 cells stained with AM1818a Beclin1 antibody.U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AM1818a Beclin1 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Beclin1 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)

Beclin 1, Monoclonal Antibody (Cat# AAA6121144)

Full Name
Beclin 1 (BECN1, GT197, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197) (Biotin)
Gene Names
BECN1; ATG6; VPS30; beclin1
Reactivity
Human
Applications
EIA, IHC, WB
Purity
Purified by Protein G Affinity Chromatography
Pricing
$780/0.1 mL | $3,505/5x0.1 mL

APG8a/b (MAP1LC3A/B), Blocking Peptide (Cat# AAA9217750)

Full Name
APG8a/b(MAP1LC3A/B) Antibody Blocking peptide
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3

ULK2, Polyclonal Antibody (Cat# AAA224302)

Full Name
RABBIT ANTI MOUSE ULK2
Gene Names
Ulk2; Unc51.2; AU015340; mKIAA0623; A830085I22Rik
Reactivity
Human (Expected from Sequence), Rat (Expected from Sequence)
Applications
WB
Pricing
$535/0.1 mg | $2,250/5x0.1 mg

ATG14, Blocking Peptide (Cat# AAA9223357)

Full Name
ATG14 Antibody (Center) Blocking peptide
Gene Names
ATG14; ATG14L; BARKOR; KIAA0831

APG8b (MAP1LC3B)-T93/Y99, Blocking Peptide (Cat# AAA9223058)

Full Name
APG8b (MAP1LC3B)-T93/Y99 Antibody (Center) Blocking peptide
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3

APG8b (MAP1LC3B), Blocking Peptide (Cat# AAA9219169)

Full Name
APG8b(MAP1LC3B) Antibody (N-term T29) Blocking peptide
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Immunofluorescence (IF) (Fluorescent image of U251 cells stained with ATG4D (N-term) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP11448a ATG4D (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (5.25 μM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Pictures were taken on a Biorevo microscope (BZ-900, Keyence). ATG4D (N-term) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)

ATG4D, Polyclonal Antibody (Cat# AAA6005420)

Full Name
ATG4D, NT (ATG4D, APG4D, AUTL4, Cysteine protease ATG4D, AUT-like 4 cysteine endopeptidase, Autophagin-4, Autophagy-related cysteine endopeptidase 4, Autophagy-related protein 4 homolog D)
Reactivity
Mouse
Applications
EL/EIA, WB, IHC
Purity
Purified
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$665/0.2 mL | $2,975/5x0.2 mL
Immunofluorescence (IF) (Fluorescent image of U251 cells stained with cleaved LC3A antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1805a cleaved LC3A primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)

MAP1LC3A, cleaved, Polyclonal Antibody (Cat# AAA6008024)

Full Name
MAP1LC3A, cleaved (LC3B, Microtubule-associated proteins 1A/1B light chain 3A, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, Microtubule-associated protein
Reactivity
Human, Mouse
Applications
EL/EIA, WB, IHC, ICC
Purity
Affinity Purified
Purified by peptide immunoaffinity chromatography.
Pricing
$685/0.2 mL | $3,065/5x0.2 mL

Autophagic-related protein 16.1 (atg-16.1), Recombinant Protein (Cat# AAA1307856)

Full Name
Recombinant Caenorhabditis elegans Autophagic-related protein 16.1 (atg-16.1), partial
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.

APG8b (MAP1LC3B) (T29), Blocking Peptide (Cat# AAA9225252)

Full Name
Phospho-APG8b(MAP1LC3B)(T29) Antibody Blocking peptide
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Immunofluorescence (IF) (Fluorescent image of U251 cells stained with ATG9A (C-term) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1814c ATG9A (C-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). ATG9A immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)

ATG9A, Polyclonal Antibody (Cat# AAA646615)

Full Name
ATG9A, CT (ATG9A, APG9L1, Autophagy-related protein 9A, APG9-like 1, mATG9)
Gene Names
ATG9A; mATG9; APG9L1; MGD3208
Reactivity
Human
Applications
EL/EIA, WB, IHC
Purity
Purified
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$675/0.2 mL | $3,020/5x0.2 mL

Autophagy-related protein 8 (atg8), Recombinant Protein (Cat# AAA1167014)

Full Name
Recombinant Aspergillus clavatus Autophagy-related protein 8 (atg8)
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,255/1 mg (E-Coli) | $1,800/1 mg (Yeast) | $2,850/1 mg (Baculovirus) | $1,435/0.1 mg (Baculovirus) | $2,060/0.1 mg (Mammalian-Cell) | $705/0.1 mg (E-Coli) | $915/0.1 mg (Yeast) | $5,700/0.5 mg (Mammalian-Cell) | $1,030/0.02 mg (Baculovirus) | $1,265/0.02 mg (Mammalian-Cell) | $605/0.02 mg (E-Coli) | $780/0.02 mg (Yeast)
Immunofluorescence (IF) (Fluorescent image of U251 cells stained with ATG7 (C-term) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1813d ATG7 (C-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). ATG7 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells, supported by Human Protein Atlas Data .)

ATG7, Polyclonal Antibody (Cat# AAA6010337)

Full Name
ATG7, CT (ATG7, APG7L, Ubiquitin-like modifier-activating enzyme ATG7, ATG12-activating enzyme E1 ATG7, Autophagy-related protein 7, Ubiquitin-activating enzyme E1-like protein)
Reactivity
Human
Applications
EL/EIA, WB, IHC
Purity
Purified
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$675/0.2 mL | $3,020/5x0.2 mL

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