Rabbit LC3 Polyclonal Antibody | anti-MAP1LC3A antibody

LC3 Antibody (APG8A) (N-term)

Cat. Num. AAA9201218

• Gene Names: MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
• Reactivity: Human, mouse, rat
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunofluorescence
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: LC3; Polyclonal Antibody; LC3 Antibody (APG8A) (N-term); Microtubule-associated proteins 1A/1B light chain 3A; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; MAP1 light chain 3-like protein 1; MAP1A/MAP1B light chain 3 A; MAP1A/MAP1B LC3 A; Microtubule-associated protein 1 light chain 3 alpha; MAP1LC3A; anti-MAP1LC3A antibody

• Host: Rabbit
• Reactivity: Human, mouse, rat
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-term of human LC3 (APG8a).
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 1-30
• Sequence Length: 125

• Applicable Applications for anti-MAP1LC3A antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of anti-LC3 (APG8a) Pab in rat brain lysate. Both lipidated (arrow, II) and non-lipidated APG8a (arrow, I) were detected in membrane fraction (P) but only non-lipidated LC3 was detected in soluble fraction (S).)

Immunofluorescence (IF)

(Fluorescent image of U251 cells stained with MBS9201218 LC3 (APG8A) (N-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9201218 LC3 (APG8A) (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with Autophagy LC3 Antibody (APG8a) (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Western Blot (WB)

(APG8a (MAP1LC3A) Antibody (M1) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the APG8a (MAP1LC3A) antibody detected the APG8a (MAP1LC3A) protein (arrow).)

Related Product Information for anti-MAP1LC3A antibody

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3a is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.

Product Categories/Family for anti-MAP1LC3A antibody

Signal Transduction; Neuroscience; Microbiology; Metabolism; Cell Biology; Cardiovascular; Cancer; Autophagy

References

References for protein:
1.Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005)
2.Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005)
3.Greenberg JT. Dev Cell. 8(6):799-801. (2005)
4.Levine B. Cell. 120(2):159-62. (2005)
5.Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004)
6.Tanida I., et al. Int. J. Biochem. Cell Biol. 36:2503-2518(2004)
7.He H., et al. J. Biol. Chem. 278:29278-29287(2003)
8.Tanida I., et al. J. Biol. Chem. 279:36268-36276(2004)
References for U251 cell line:
1. Westermark B.; Pontén J.; Hugosson R. (1973)." Determinants for the establishment of permanent tissue culture lines from human gliomas". Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449].
2. Pontén, J.,Westermark B. (1978)." Properties of Human Malignant Glioma Cells in Vitro". Medical Biology 56: 184-193.[PMID: 359950].
3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). "Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent". Neuro Oncol. 12(5): 473-481.[ PMID: 20406898].

NCBI and Uniprot Product Information

• NCBI GI #: 14210522
• NCBI GeneID: 84557
• NCBI Accession #: NP_115903.1; NP_852610.1
• NCBI GenBank Nucleotide #: NM_032514.3
• UniProt Accession #: Q9H492; Q9BXW5; E1P5P4; E1P5P5
• Molecular Weight: 14272
• NCBI Official Full Name: microtubule-associated proteins 1A/1B light chain 3A isoform a
• NCBI Official Synonym Full Names: microtubule-associated protein 1 light chain 3 alpha
• NCBI Official Symbol: MAP1LC3A
• NCBI Official Synonym Symbols: LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
• NCBI Protein Information: microtubule-associated proteins 1A/1B light chain 3A
• UniProt Protein Name: Microtubule-associated proteins 1A/1B light chain 3A
• Protein Family: Microtubule-associated protein
• UniProt Gene Name: MAP1LC3A
• UniProt Synonym Gene Names: MAP1A/MAP1B LC3 A
• UniProt Entry Name: MLP3A_HUMAN

NCBI Description

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis. [provided by RefSeq, Feb 2012]

Uniprot Description

LC3A: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle; Microtubule-binding; Autophagy; Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 20q11.22

Cellular Component: microtubule; extrinsic to membrane; late endosome; autophagic vacuole; cytoplasmic vesicle; cytosol

Molecular Function: protein binding; phosphatidylethanolamine binding; microtubule binding; GABA receptor binding; phospholipid binding

Biological Process: mitochondrion degradation; autophagic vacuole formation; cellular response to nitrogen starvation