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48 results for " Cas9 CRISPR" - showing 1-48
Western Blot (WB)
CRISPR/Cas9, Monoclonal Antibody (Cat# AAA375383)
Full Name
Mouse Anti- CRISPR/Cas9 [7A9-3A3]
Reactivity
Streptococcus pyogene
Applications
IP, IF
Purity
Protein A
Pricing
$510/0.1 mL | $2,140/5x0.1 mL
Western Blot (WB)
(Fig-1: Detection of Cas9 in the HEK293/Cas9 stable cell line by Western blotting using anti-Cas9 antibody.)
Cas9, Cell Line (Cat# AAA669111)
Full Name
Cas9 Stable Cell Line
Applications
WB, IP
Pricing
$2,845/1 Vial | $12,730/5x1 Vial
S. Pyogenes CRISPR-Associated Protein-9 Nuclease, Recombinant Protein (Cat# AAA140642)
Full Name
Recombinant S. Pyogenes CRISPR-Associated Protein-9 Nuclease
Purity
Greater than 97% as determined by SDS-PAGE.
Pricing
$865/0.05 mg | $1,415/0.1 mg | $4,125/0.5 mg | $18,350/5x0.5 mg
Typical Testing Data/Standard Curve (for reference only)
Cas9 (CRISPR Associated Protein 9), ELISA Kit (Cat# AAA169377)
Full Name
Cas9 (CRISPR Associated Protein 9) ELISA Kit
Pricing
$630/96 Assays | $2,880/5x96 Assays
Testing Data
Cas9 Nuclease GFP NLS, Enzyme (Cat# AAA4156509)
Full Name
Cas9 Nuclease GFP NLS Protein
Pricing
$165/0.0095 mg | $510/5x0.0095 mg
Western Blot (WB)
(Western blot analysis of CRISPR-Cas9 SP expression in (1) 293T cell lysate transfected with CRISPR-Cas9; (2) 293T cell lysate; (3) 3T3 cell lysate; (4) PC12 cell lysate (MBS1750147).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-cas9 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for cas9 )
CRISPR-Cas9 SP, Monoclonal Antibody (Cat# AAA1750147)
Full Name
Anti-CRISPR-Cas9 SP Rabbit Monoclonal Antibody
Reactivity
Streptococcus pygenes
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
CRISPR-Cas9, Monoclonal Antibody (Cat# AAA8534487)
Full Name
CRISPR-Cas9 SP
Reactivity
Streptococcus pyogenes
Applications
WB, ICC, IF, IHC, FC/FACS
Purity
ProA affinity purified
Pricing
$350/0.1 mL | $570/0.1 mL (Biotin) | $1,515/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis on cas9 transfected 293 cells (+) and non trasfected 293 cells (-))
CRISPR-Cas9, Polyclonal Antibody (Cat# AAA8534771)
Full Name
CRISPR-Cas9
Applications
WB, EIA, ICC
Purity
Immunogen affinity purified
Pricing
$350/0.2 mL | $570/0.1 mL (Biotin) | $1,515/5x0.2 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB)
(Western blot of GFP-Cas9 transfected HEK293 cell lysate (+) showing specific immunolabeling of the ~53 kDa GFP-Cas9 fusion protein. Labeling is absent in the untransfected lysate (-).)
CRISPR Cas9, Monoclonal Antibody (Cat# AAA500083)
Full Name
Anti-CRISPR Cas9 Antibody
Applications
WB, IF, IHC
Purity
Protein G Purified Culture Supernatant
Pricing
$420/0.1 mL | $1,740/5x0.1 mL
Western Blot (WB)
(Western blot analysis of CRISPR-Cas9 expression in (1) 293T cell lysate transfected with CRISPR-Cas9; (2) 293T cell lysate; (3) 3T3 cell lysate; (4) PC12 cell lysate (MBS1750148).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-cas9 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for cas9 )
CRISPR-Cas9, Monoclonal Antibody (Cat# AAA1750148)
Full Name
Anti-CRISPR-Cas9 Rabbit Monoclonal Antibody
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
CRISPR-Cas9, Monoclonal Antibody (Cat# AAA8533559)
Full Name
CRISPR-Cas9
Applications
WB, ICC
Purity
ProA affinity purified
Pricing
$350/0.1 mL | $570/0.1 mL (Biotin) | $1,515/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
CRISPR-Cas9, Monoclonal Antibody (Cat# AAA9457102)
Full Name
CRISPR-Cas9 SP Conjugated Antibody
Reactivity
Streptococcus pyogenes
Applications
Most Applications
Pricing
$480/0.1 mL (Biotin) | $480/0.1 mL (AF350) | $480/0.1 mL (AF405) | $480/0.1 mL (AF488) | $480/0.1 mL (AF555) | $480/0.1 mL (AF594) | $480/0.1 mL (AF647) | $480/0.1 mL (AF680) | $480/0.1 mL (AF750)
serotype M1 cas9, Recombinant Protein (Cat# AAA156830)
Full Name
Recombinant Streptococcus pyogenes serotype M1 cas9 protein, C-His Tag
Purity
>90% as determined by SDS-PAGE
Pricing
$310/0.05 mg | $400/0.1 mg | $1,860/1 mg | $8,080/5x1 mg
CAS9, Polyclonal Antibody (Cat# AAA8560377)
Full Name
CAS9
Reactivity
Streptococcus Pyogenes
Applications
EIA, IHC
Purity
Purified By Antigen Affinity Column
Pricing
$350/0.2 mL | $570/0.1 mL (Biotin) | $1,515/5x0.2 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
CRISPR-Cas9, Polyclonal Antibody (Cat# AAA8543300)
Full Name
CRISPR-Cas9 Rabbit pAb
Reactivity
Staphylococcus aureus
Applications
WB, IHC, ICC, IF, IP, FC/FACS
Purity
Affinity Chromatography
Pricing
$310/0.1 mL | $570/0.1 mL (Biotin) | $1,330/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis on cas9 transfected 293 cells (+) and non transtected 293 cells (-).)
CRISPR-Cas9, Polyclonal Antibody (Cat# AAA9435071)
Full Name
CRISPR-Cas9 Antibody
Applications
WB
Purity
Immunogen affinity purified
Pricing
$325/0.05 mL | $445/0.1 mL | $1,860/5x0.1 mL
Western Blot (WB)
(Western Blot (WB) analysis of CRISPR/Cas9 (R780) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysate. Lane2:HEK293T transfected with CRISPR/Cas9 (L:P=3:1). Lane3:HEK293T transfected with CRISPR/Cas9 (L:P=6:1). Lane4:HEK293T transfected with CRISPR/Cas9 (L:P=9:1).)
CRISPR/Cas9, Polyclonal Antibody (Cat# AAA3000285)
Full Name
CRISPR/Cas9 (R780) Polyclonal Antibody
Reactivity
Recombinant Cas9
Applications
WB
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Pricing
$310/0.05 mL | $435/0.1 mL | $1,695/5x0.1 mL
serotype M1 cas9, Recombinant Protein (Cat# AAA156814)
Full Name
Recombinant Streptococcus pyogenes serotype M1 cas9 protein, N-His Tag
Purity
>90% as determined by SDS-PAGE
Pricing
$310/0.05 mg | $400/0.1 mg | $1,860/1 mg | $8,080/5x1 mg
Testing Data
saCas9 Nuclease NLS, Recombinant Protein (Cat# AAA4160527)
Full Name
saCas9 Nuclease NLS Protein
Pricing
$165/0.0065 mg | $510/5x0.0065 mg
CRISPR-Cas9, Polyclonal Antibody (Cat# AAA8534796)
Full Name
CRISPR-Cas9
Applications
WB, EIA, ICC
Purity
ProA affinity purified
Pricing
$350/0.2 mL | $570/0.1 mL (Biotin) | $1,515/5x0.2 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB)
(Western Blot analysis of CRISPR-Cas9 SP on 293 and 293T cells lysates transfected with CRISPR-Cas9 using anti- CRISPR-Cas9 SP antibody at 1/500 dilution.)
CRISPR-Cas9 SP, Polyclonal Antibody (Cat# AAA3012510)
Full Name
CRISPR-Cas9 SP Polyclonal Antibody
Reactivity
Streptococcus pyogenes
Applications
WB, ICC, IF, IHC, FC/FACS
Purity
ProA affinity purified
Pricing
$355/0.05 mL | $470/0.1 mL | $1,855/5x0.1 mL
Western Blot (WB)
(Western blot analysis of CRISPR-Cas9 SP on 293 and 293T cells lysates transfected with CRISPR-Cas9 using anti- CRISPR-Cas9 SP antibody at 1/500 dilution.)
CRISPR-Cas9, Monoclonal Antibody (Cat# AAA9436243)
Full Name
CRISPR-Cas9 SP Antibody
Reactivity
Streptococcus pyogenes
Applications
WB, ICC, IF, IHC, FC/FACS
Purity
ProA affinity purified
Pricing
$325/0.05 mL | $445/0.1 mL | $1,860/5x0.1 mL
Western Blot (WB)
(SaCas9 antibody (mAb) tested by Western blot. Lane 1: OSC Drosophila ovary cell lysate, transfected with SaCas9 (WT); Lane 2: OSC cell lysate transfected with SaCas9 (DN).)
SaCas9, Monoclonal Antibody (Cat# AAA389045)
Full Name
SaCas9 mAb (Clone 11E7), sample
Reactivity
Not Species Specific
Applications
WB
Purity
Protein A Chromatography
Pricing
$200/0.01 mg | $590/0.1 mg | $2,595/5x0.1 mg
CRISPR-Cas9 SP, Polyclonal Antibody (Cat# AAA8543301)
Full Name
CRISPR-Cas9 SP Rabbit pAb
Reactivity
Streptococcus Pyogenes
Applications
WB, IHC, ICC, IF, FC/FACS
Purity
Affinity Chromatography
Pricing
$310/0.1 mL | $570/0.1 mL (Biotin) | $1,330/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
serotype M1 cas9, Recombinant Protein (Cat# AAA156813)
Full Name
Recombinant Streptococcus pyogenes serotype M1 cas9 protein, N-His Tag
Purity
>90% as determined by SDS-PAGE
Pricing
$310/0.05 mg | $400/0.1 mg | $1,860/1 mg | $8,080/5x1 mg
HeLa/Cas9, Cell Line (Cat# AAA169562)
Full Name
HeLa/Cas9 Cell Line
Pricing
$615/1 Vial | $2,575/5x1 Vial
Western Blot (WB)
(Western Blot (WB) analysis of CRISPR/Cas9 (P860) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysate. Lane2:HEK293T transfected with CRISPR/Cas9.)
CRISPR/Cas9, Polyclonal Antibody (Cat# AAA3000286)
Full Name
CRISPR/Cas9 (P860) Polyclonal Antibody
Reactivity
Recombinant Cas9
Applications
WB
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Pricing
$310/0.05 mL | $435/0.1 mL | $1,695/5x0.1 mL
Western Blot (WB)
(Western Blot (WB) analysis of CRISPR/Cas9 (D1100) polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysate. Lane2:HEK293T transfected with CRISPR/Cas9.)
CRISPR/Cas9, Polyclonal Antibody (Cat# AAA3000287)
Full Name
CRISPR/Cas9 (D1100) Polyclonal Antibody
Reactivity
Recombinant Cas9
Applications
WB
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Pricing
$310/0.05 mL | $435/0.1 mL | $1,695/5x0.1 mL
Western Blot (WB)
(Western Blot (WB) analysis of CRISPR/Cas9 Mouse mAb at 1:5000 dilutionLane 1: CRISPR/Cas9 transfected 293 cells lysate Lane 2: Non-transfected 293 cells lysate)
CRISPR-Cas9, Monoclonal Antibody (Cat# AAA3014428)
Full Name
CRISPR-Cas9 Mouse Monoclonal Antibody
Reactivity
Recombinant Cas9
Applications
WB, ICC
Purity
Protein A affinity purified
Pricing
$355/0.05 mL | $470/0.1 mL | $1,855/5x0.1 mL
CAS9, Polyclonal Antibody (Cat# AAA7144677)
Full Name
Rabbit anti-Streptococcus pyogenes serotype M1 CAS9 Polyclonal Antibody
Reactivity
Streptococcus pyogenes serotype M1
Applications
EIA, Western Blot, ensure identification of antigen
Purity
Protein A/G
NIH3T3/Cas9, Cell Line (Cat# AAA169545)
Full Name
NIH3T3/Cas9 Cell Line
Pricing
$615/1 Vial | $2,575/5x1 Vial
293/Cas9, Cell Line (Cat# AAA169561)
Full Name
293/Cas9 Cell Line
Pricing
$615/1 Vial | $2,575/5x1 Vial
Testing Data
saCas9 Nuclease NLS, Recombinant Protein (Cat# AAA4160532)
Full Name
saCas9 Nuclease NLS Protein
Pricing
$540/0.15 mg | $2,185/5x0.15 mg
SDS-Page
serotype M1 cas9, Recombinant Protein (Cat# AAA157486)
Full Name
Recombinant Streptococcus pyogenes serotype M1 cas9 protein, N- His tag
Purity
>90% as determined by SDS-PAGE
Pricing
$310/0.05 mg | $400/0.1 mg | $1,510/5x0.1 mg
Testing Data
Cas9 Nuclease NLS Protein, Protein (Cat# AAA4160529)
Full Name
Cas9 Nuclease NLS Protein
Pricing
$185/0.04 mg | $595/5x0.04 mg
AM18-10LB-B cas9, Recombinant Protein (Cat# AAA1563020)
Full Name
Recombinant Eubacterium sp. AM18-10LB-B cas9 protein, N-His Tag
Purity
>90% as determined by SDS-PAGE
Pricing
$310/0.05 mg | $400/0.1 mg | $1,510/5x0.1 mg
serotype M1 cas9, Recombinant Protein (Cat# AAA157637)
Full Name
Recombinant Streptococcus pyogenes serotype M1 cas9 protein, N- His Tag
Purity
>90% as determined by SDS-PAGE
Pricing
$310/0.05 mg | $400/0.1 mg | $1,510/5x0.1 mg
Western Blot (WB)
(Cas9 antibody (mAb) tested by Immunoprecipitation. 5 ug of Cas9 antibody (mAb) was used to immunoprecipitate Cas9 from 100 ug of whole cell extracts after 72 hours of transient transfection of HEK293 cells with DYKDDDDK-Tagged Cas9. The immunoprecipitated protein was detected by Western blotting using Cas9 antibody (mAb) at a 0.5 ug/mL dilution.)
Cas9, Monoclonal Antibody (Cat# AAA388960)
Full Name
Cas9 antibody (mAb)(Clone 7A9-3A3)
Reactivity
Human
Applications
ChIP, ICC, IF, IP, WB
Purity
Protein G Chromatography
Pricing
$200/0.01 mg | $375/0.05 mg | $600/0.1 mg | $2,645/5x0.1 mg
Streptococcus pyogenes M1 CRISPR-Cas9, Cell Lysate (Cat# AAA8116161)
Full Name
Streptococcus pyogenes M1 CRISPR-Cas9 Baculovirus-Insect Overexpression Lysate
Applications
WB
Pricing
$280/0.3 mg | $1,205/5x0.3 mg
CAS9, Polyclonal Antibody (Cat# AAA7145761)
Full Name
Rabbit anti-Streptococcus thermophilus CAS9 Polyclonal Antibody
Reactivity
Streptococcus thermophilus
Applications
EIA, Western Blot, ensure identification of antigen
Purity
Protein A/G
Structure
RS-1, Biochemical (Cat# AAA3604000)
Full Name
RS-1
Purity
>98% (HPLC)
Pricing
$215/10 mg | $340/50 mg | $480/100 mg | $1,870/5x100 mg
Testing Data
saCas9 Nuclease NLS, Recombinant Protein (Cat# AAA4160533)
Full Name
saCas9 Nuclease NLS Protein
Pricing
$225/0.03 mg | $785/5x0.03 mg
Testing Data
saCas9 Nuclease NLS, Recombinant Protein (Cat# AAA4160531)
Full Name
saCas9 Nuclease NLS Protein
Pricing
$245/0.03 mg | $880/5x0.03 mg
Testing Data
Cas9 Nuclease NLS Protein, Protein (Cat# AAA4160530)
Full Name
Cas9 Nuclease NLS Protein
Pricing
$315/0.2 mg | $1,160/5x0.2 mg
SDS-Page
(Recombinant Cas9 protein gel 7.5% SDS-PAGE gel stained with Coomassie Blue. MW: 161.2 kDa Purity: > 85% )
Cas9, Recombinant Protein (Cat# AAA389126)
Full Name
Recombinant Cas9 protein
Pricing
$490/0.1 mg | $3,175/1 mg | $14,240/5x1 mg
SDS-Page
(AbFlex SaCas9 antibody (rAb) tested by Western blot. 20 ug of HeLa whole-cell extract (Lane 1) and HeLa whole-cell extract with 10 ng of full length SaCas9 protein (Lane 2), was run on SDS-PAGE and probed with AbFlex SaCas9 antibody at 0.5 ug/ml.)
SaCas9, Antibody (Cat# AAA389497)
Full Name
AbFlex SaCas9 antibody (rAb)
Reactivity
Human
Applications
WB
Purity
Protein A Chromatography
Pricing
$160/0.01 mg | $460/0.1 mg | $2,020/5x0.1 mg
SDS-Page
(AbFlex Cas9 antibody (rAb) tested by Western blot. 50 ng recombinant Cas9 protein was probed with 0.5 ug/ml AbFlex Cas9 antibody (Lane 1). HEK293T cells were transfected with a plasmid containing a mammalian expression construct for dCas9 (S. pyogenes). Chromatin was prepared 48 hours post-transfection, boiled and 15 ul (~200,000 cell equivalents) was run on a SDS-PAGE gel and probed with 2 ug/ml AbFlex Cas9 antibody (Lane 2). A molecular weight marker was run in Lane 3.)
Cas9, Antibody (Cat# AAA389444)
Full Name
AbFlex Cas9 antibody (rAb)
Reactivity
Human
Applications
ChIP, ChIP-Seq, EIA, WB
Purity
Ni-NTA
Pricing
$160/0.01 mg | $460/0.1 mg | $2,020/5x0.1 mg
Chromatin Immunoprecipitation (ChIP)
(Cas9 antibody (mAb) tested by ChIP. NIH3T3 cells stably expressing GFP-H2B, nuclease dead Cas9, and a GFP-targeting gRNA were fixed with formaldehyde, harvested and sonicated to get 200-500bp DNA fragments. 50?g chromatin was incubated O/N at 4 degree C with Cas9 antibody (200?l hybridoma SN, 5?g a-DYKDDDDK) followed by incubation with protein G beads for 3h at 4 degree C. After washing chromatin was eluted from the beads and crosslinking was reversed )/N at 65 degree C. After a proteinase K digesting step DNA was separated using phenol/chloroform/isoamyl alcohol, precipitated with ethanol/sodium acetate and dissolved in water. For the qPCR primers either targeting the GFP gene or as negative control non-targeted regions (Ppap2c +7122 and Prkcd +24069 from transcription start) were used.)
Cas9, Monoclonal Antibody (Cat# AAA389038)
Full Name
Cas9 antibody (mAb)(Clone 8C1-F10)
Reactivity
Human, Mouse
Applications
ChIP, IF, IP, WB
Purity
Protein A Chromatography
Pricing
$200/0.01 mg | $375/0.05 mg | $600/0.1 mg | $2,645/5x0.1 mg
