Testing Data
Cas9 Nuclease GFP NLS Enzyme
Cas9 Nuclease GFP NLS Protein
Synonyms
Cas9 Nuclease GFP NLS; Cas9 Nuclease GFP NLS Protein; spCas9; spCas9-GFP; Cas9-GFP; CRISPR-associated endonuclease Cas9 from Streptococcus pyogenes; Cas9 Nuclease GFP NLS enzyme
Host
E. coli
Form/Format
Protein Storage Buffer: 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v/v) Glycerol.
10X Cas9 Reaction Buffer Components: 200 mM HEPES, 50 mM MgCl2, 1 M NaCl,1 mM EDTA, pH 6.5.
10X Cas9 Reaction Buffer Components: 200 mM HEPES, 50 mM MgCl2, 1 M NaCl,1 mM EDTA, pH 6.5.
Concentration
Protein Concentration: 1000nM, 188 ug/mL (varies by lot)
Includes
- Cas9 Nuclease GFP NLS Protein (1000 nM) / Size: 9.5 ug (50 pmol/50 ul)
- 10X Cas9 Reaction Buffer / Size: 1.25 mL
- 10X Cas9 Reaction Buffer / Size: 1.25 mL
Cas Type
Nuclease
Cas Origin
spCas9
Cas Protein Marker
GFP
Preparation and Storage
Store all components at -20°C.
Testing Data
Testing Data
Testing Data
Testing Data
Related Product Information for Cas9 Nuclease GFP NLS enzyme
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) /Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. The Cas9 nuclease serves to unwind the genomic DNA duplex next to conserved protospacer adjacent motifs (PAMs) and homes in on its target sequence, which is recognized by a complementary single-guide RNA. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene. Alternatively, by supplying a suitable repair template, virtually any desired point mutation can be introduced at the break point via homology-directed repair (HDR). The Cas9 from the bacteria Streptococcus pyogenes, abbreviated spCas9, is the most commonly used Cas9 variant. The fusion of Cas9 Nuclease NLS to GFP allows for visual confirmation of transfection as well as subsequent verification of Cas9 clearance from the cells. Cas9 Nuclease-GFP can also be used for FACS applications and screening. Cas9 Nuclease-GFP NLS contains a SV40 T antigen nuclear localization sequence (NLS) on the C-terminus of the protein. Our Cas9-GFP proteins have the Enhanced green fluorescent protein (eGFP).
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Product Notes
The Cas9 Nuclease GFP NLS (Catalog #AAA4156509) is an Enzyme produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Cas9 Nuclease GFP NLS, Enzyme" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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