S. Pyogenes CRISPR-Associated Protein-9 Nuclease Recombinant Protein | CAS9 recombinant protein

Recombinant S. Pyogenes CRISPR-Associated Protein-9 Nuclease

Cat. Num. AAA140642

• Purity: Greater than 97% as determined by SDS-PAGE.
• Synonyms: S. Pyogenes CRISPR-Associated Protein-9 Nuclease; Recombinant S. Pyogenes CRISPR-Associated Protein-9 Nuclease; CAS9 S. Pyogenes; CRISPR-Associated Protein-9 Nuclease S. Pyogenes Recombinant; CRISPR-associated endonuclease Cas9/Csn1; SpyCas9; cas9; csn1; CAS9 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 97% as determined by SDS-PAGE.
• Form/Format: The CAS9 solution (1mg/ml) contains 10mM Tris-Cl, 250mM NaCl, 0.1mM EDTA, 50% glycerol and 1mM DTT pH 7.5.; Sterile Filtered colorless solution.
• Sequence Length: 1368

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Related Product Information for CAS9 recombinant protein

Introduction: Cas9 (CRISPR associated protein 9) is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in Streptococcus pyogenes, among other bacteria. S. Pyogenes utilizes Cas9 to remember and later probe and cleave foreign DNA, such as invading bacteriophage DNA or plasmid DNA. In case that the DNA substrate is complementary to the guide RNA, Cas9 cleaves the invading DNA. In addition to its original role in bacterial immunity, the Cas9 protein has been heavily employed as a genome engineering tool to induce site-directed double strand breaks in DNA. These disruptions may lead to gene inactivation or the presentation of heterologous genes via non-homologous end joining and homologous recombination respectively in many laboratory model organisms.

Description: Recombinant Cas9-NLS comprises the entire Cas9 protein sequence (1368 amino acids) joined to a 6xHis tag at the N-terminus and a proprietary nuclear localization sequence (NLS) at the C-terminus. The protein has an apparent molecular weight of 160kDa on SDS-PAGE.

Product Categories/Family for CAS9 recombinant protein

Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 15675041
• NCBI GeneID: 901176
• NCBI Accession #: NP_269215.1
• NCBI GenBank Nucleotide #: NC_002737.2
• UniProt Accession #: Q99ZW2
• Molecular Weight: 158,441 Da
• NCBI Official Full Name: conserved hypothetical protein
• NCBI Official Symbol: SPy_1046
• NCBI Protein Information: hypothetical protein
• UniProt Protein Name: CRISPR-associated endonuclease Cas9/Csn1
• Protein Family: CRISPR-associated endonuclease
• UniProt Gene Name: cas9
• UniProt Entry Name: CAS9_STRP1

Uniprot Description

Cas9: CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA probably requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and Cas9. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA (By similarity). Cas9/crRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. Binding to dsDNA target requires a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif). When the CRISPR3/cas system consisting of cas9-cas1-cas2-csn2-CRISPR3 or just cas9-CRISPR3 is expressed in E.coli it prevents plasmids homologous to spacers 1 or 2 from transforming

Protein type: EC 3.1.-.-