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10000 results for " phosphorylation" - showing 4600-4650
FAK, Polyclonal Antibody (Cat# AAA627821)
Full Name
FAK, phosphorylated (Tyr925) (Focal Adhesion Kinase 1, FADK 1, pp125FAK, Protein-tyrosine Kinase 2, PTK2, FAK1)
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$465/0.05 mg | $2,090/5x0.05 mg
Western Blot (WB)
(Western blot analysis of extracts from A431 cells untreated or treated with EGF (200ng/ml, 5min), using CARM1 (Ab-228) antibody and CARM1 (Phospho-Ser228) antibody.)
CARM1, Antibody (Cat# AAA857509)
Full Name
CARM1 (Ab-228) Antibody
Gene Names
CARM1; PRMT4
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$195/0.05 mg | $255/0.1 mg | $1,100/5x0.1 mg | $820/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of extracts from 293 cells treated with PMA using Phospho-C-RAF (Ser296) antibody.The lane on the right is treated with the antigen-specific peptide.)
C-RAF, Polyclonal Antibody (Cat# AAA9403142)
Full Name
C-RAF (Phospho-Ser296) Antibody
Gene Names
RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
BCL2L11, Polyclonal Antibody (Cat# AAA1488382)
Full Name
Rabbit Anti Human BCL2L11 PolyClonal Antibody
Gene Names
BCL2L11; BAM; BIM; BOD
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$325/0.1 mL | $1,465/5x0.1 mL
Western Blot (WB)
(Western Blot analysis of Mouse cells with Phospho-Ephrin-B1 (Tyr317) Polyclonal Antibody )
Ephrin-B1, Polyclonal Antibody (Cat# AAA2535969)
Full Name
Phospho-Ephrin-B1 (Y317) Polyclonal Antibody
Gene Names
EFNB1; CFND; CFNS; EFB1; EFL3; EPLG2; Elk-L; LERK2
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Antigen Affinity Purified
Pricing
$130/0.02 mL | $395/0.2 mL | $250/0.12 mL | $170/0.06 mL | $1,715/5x0.2 mL
Testing Data
(Detection of phospho-CREB in HEK 293T cells using MBS631087 (top panel). Bottom panel: HEK 293T cells probled with CREB antibody. Lanes 2 and 4 (top and bottom): Samples treated with lambda-Phosphatase. Lanes 3 and 4 (top and bottom): Samples treated with 0.2mM H2O2.)
CREB, Polyclonal Antibody (Cat# AAA631087)
Full Name
CREB, phosphorylated (Ser121) (CBP, CREBBP, CREB binding protein, cAMP Response Element Binding Protein, RTS, RSTS, Rubinstein-Taybi syndrome)
Gene Names
CREB1; CREB; MGC9284
Reactivity
Human, Mouse
Applications
WB, IP
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$610/0.1 mL | $2,730/5x0.1 mL
Western Blot (WB)
(Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using ABL1 (Phospho-Thr735) Antibody. The lane on the right is blocked with the phospho peptide.)
ABL1, Polyclonal Antibody (Cat# AAA9503235)
Full Name
ABL1 (Phospho-Thr735) Antibody
Gene Names
ABL1; ABL; JTK7; p150; c-ABL; v-abl; CHDSKM; c-ABL1; bcr/abl
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Pricing
$265/0.05 mL | $365/0.1 mL | $1,580/5x0.1 mL
GluR1, Polyclonal Antibody (Cat# AAA629553)
Full Name
GluR1, phosphorylated (Ser831) (Glutamate Receptor)
Gene Names
GRIA1; GLUH1; GLUR1; GLURA; GluA1; HBGR1; MGC133252
Reactivity
Rat
Applications
WB, GS/EMSA
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$860/0.15 mL | $3,860/5x0.15 mL
ZAP70, Recombinant Protein (Cat# AAA5303958)
Full Name
ZAP70 protein
Gene Names
ZAP70; SRK; STD; TZK; STCD; ZAP-70
Purity
ZAP70 protein was purified using a a proprietary chromatographic technique.
Pricing
$555/0.005 mg | $2,490/5x0.005 mg
TSC2, Polyclonal Antibody (Cat# AAA623439)
Full Name
TSC2, phosphorylated (Ser1254) (Tuberin, Tuberous Sclerosis 2 Protein, TSC4)
Gene Names
TSC2; LAM; TSC4
Reactivity
Human
Applications
EL/EIA, WB, IHC
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$465/0.05 mg | $2,090/5x0.05 mg
RIPK1, Blocking Peptide (Cat# AAA544377)
Full Name
RIPK1 Antibody Antigenic Blocking Peptide
Gene Names
RIPK1; RIP; RIP1; RIP-1
Applications
Immunodepletion, Immunocompetition
Pricing
$280/0.25 mg | $1,110/5x0.25 mg
Immunohistochemistry (IHC)
(Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD (Ab-112) antibody.)
BAD, Antibody (Cat# AAA857069)
Full Name
BAD (Ab-112) Antibody
Gene Names
BAD; BBC2; BCL2L8
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$195/0.05 mg | $255/0.1 mg | $1,100/5x0.1 mg | $820/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot All lanes: Protein AATF antibody at at 2 /mlLane 1: 293T whole cell lysateLane 2: EC109 whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 62kDa Observed band size: 50kDa Additional bands at: 100kDa?10 kDa. We are unsure as to the identity of these extra bands. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Protein AATF, Polyclonal Antibody (Cat# AAA715767)
Full Name
Rabbit anti-human Protein AATF polyclonal Antibody, Biotin conjugated
Gene Names
AATF; DED; BFR2; CHE1; CHE-1
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts from K562 cells treated with EGF using B-Raf (Phospho-Thr753) Antibody.The lane on the right is treated with the antigen-specific peptide.)
B-Raf, Polyclonal Antibody (Cat# AAA9413283)
Full Name
B-Raf (Phospho-Thr753) Antibody
Gene Names
BRAF; NS7; BRAF1; RAFB1; B-RAF1
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Testing Data
(P-peptide-+)
BLNK, Antibody (Cat# AAA855772)
Full Name
BLNK (Phospho-Tyr84) Antibody
Gene Names
BLNK; bca; AGM4; BASH; LY57; SLP65; BLNK-S; SLP-65
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot V-type proton ATPase subunit G 1 antibody at at 2 /ml+ 293T whole cell lysate at 20 SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 13 kDa Observed band size: 50kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
V-type proton ATPase subunit G 1, Polyclonal Antibody (Cat# AAA715298)
Full Name
Rabbit anti-human V-type proton ATPase subunit G 1 polyclonal Antibody, FITC
Gene Names
ATP6V1G1; ATP6G; ATP6J; Vma10; ATP6G1; ATP6GL
Reactivity
Human, Mouse, Rat, Cow
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Immunohistochemistry (IHC)
(MBS630068 staining on Normal Cerebellum. Tissue pretreated with Citrate, pH 6.0. The antibody was diluted to 1:200, using IHC-Select Detection with HRP-DAB.)
Neurofilament M, 145 kD, Polyclonal Antibody (Cat# AAA630068)
Full Name
Neurofilament M, 145 kD, CT (NF-M)
Reactivity
Bovine, Chicken, Human, Mouse, Porcine, Rat
Applications
WB, IHC
Purity
Serum
Neat serum
Neat serum
Pricing
$690/0.05 mL | $3,105/5x0.05 mL
Western Blot (WB)
(Western blot analysis of extracts from HepG2 cells, using TPH2 (Phospho-Ser19) antibody.)
TPH2, Antibody (Cat# AAA858310)
Full Name
TPH2 (Phospho-Ser19) Antibody
Gene Names
TPH2; NTPH; ADHD7
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
((0.5ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
DYRK1A, Polyclonal Antibody (Cat# AAA423127)
Full Name
Goat anti-DYRK1A Antibody
Gene Names
DYRK1A; MNB; DYRK; HP86; MNBH; MRD7; DYRK1
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Applications
EIA, WB
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg
Western Blot (WB)
(Western Blot analysis of PC-3 cells using Phospho-PPAR-Gamma (S112) Polyclonal Antibody)
PPAR-gamma, Polyclonal Antibody (Cat# AAA9402552)
Full Name
PPAR-gamma (Phospho-Ser112) Polyclonal Antibody
Gene Names
PPARG; GLM1; CIMT1; NR1C3; PPARG1; PPARG2; PPARgamma
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Western Blot (WB)
(Western Blot from A2780 cells using MBS623301)
cdc25A, Polyclonal Antibody (Cat# AAA623301)
Full Name
cdc25A, phosphorylated (Ser75)
Reactivity
Human
Applications
WB, IHC
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$550/0.1 mg | $2,455/5x0.1 mg
Testing Data
Elk-1, Polyclonal Antibody (Cat# AAA616209)
Full Name
Elk-1, phosphorylated (Ser389)
Reactivity
Human
Applications
IHC
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$550/0.1 mg | $2,455/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts from MOLT cells using Rb (Ab-795) antibody.)
Rb, Antibody (Cat# AAA856988)
Full Name
Rb (Ab-795) Antibody
Gene Names
RB1; RB; pRb; OSRC; pp110; p105-Rb; PPP1R130
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$195/0.05 mg | $255/0.1 mg | $1,100/5x0.1 mg | $820/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Lamin A/B antibody)
Lamin A/B, Polyclonal Antibody (Cat# AAA5302115)
Full Name
Lamin A/B antibody
Gene Names
lmna; lmna-A
Applications
EIA, IHC, IF, WB
Purity
Lamin A/B antibody was purified by affinity chromatography
Pricing
$405/0.1 mg | $1,805/5x0.1 mg
Immunohistochemistry (IHC)
(Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK1 (Ab-291) antibody.)
MEK1, Antibody (Cat# AAA856904)
Full Name
MEK1 (Ab-291) Antibody
Gene Names
MAP2K1; CFC3; MEK1; MKK1; MAPKK1; PRKMK1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$195/0.05 mg | $255/0.1 mg | $1,100/5x0.1 mg | $820/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of extracts from 293cells, treated with EGF (200ng/ml, 30mins), using FAK (Phospho-Tyr397) antibody.)
FAK, Antibody (Cat# AAA855472)
Full Name
FAK (Phospho-Tyr397) Antibody
Gene Names
PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Immunohistochemistry (IHC)
(P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using SEK1/MKK4 (phospho-Ser80) antibody.)
SEK1/MKK4, Antibody (Cat# AAA857439)
Full Name
SEK1/MKK4 (Phospho-Ser80) Antibody
Gene Names
MAP2K4; JNKK; MEK4; MKK4; SEK1; SKK1; JNKK1; SERK1; MAPKK4; PRKMK4; SAPKK1; SAPKK-1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$235/0.05 mg | $310/0.1 mg | $1,330/5x0.1 mg | $1,015/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot detection of STAT1 in Hela, MCF7, COS7, SW480 and A549 cell lysates using STAT1 mouse mAb (1:1000 diluted).Predicted band size:91KDa.Observed band size:91KDa.)
STAT1, Monoclonal Antibody (Cat# AAA475231)
Full Name
Anti-STAT1 Mouse mAb
Gene Names
STAT1; CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91
Reactivity
Human, Monkey
Applications
WB, IP
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,520/5x0.1 mL
FAK, Polyclonal Antibody (Cat# AAA628783)
Full Name
FAK, phosphorylated (Tyr397) (Focal Adhesion Kinase 1, FADK 1, pp125FAK, Protein-tyrosine Kinase 2, PTK2, FAK1)
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$465/0.05 mg | $2,090/5x0.05 mg
Immunohistochemistry (IHC)
(P-peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cortactin (phospho-Tyr466) antibody.)
Cortactin, Antibody (Cat# AAA853450)
Full Name
Cortactin (Phospho-Tyr466) Antibody
Gene Names
CTTN; EMS1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Testing Data
(P-peptide-+)
AKT1/2/3, Antibody (Cat# AAA853798)
Full Name
AKT1/2/3 (Phospho-Tyr315/316/312) Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
IHC, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
CDK6, Polyclonal Antibody (Cat# AAA629853)
Full Name
CDK6, phosphorylated (Tyr24) (Cell Division Protein Kinase 6, Serine/Threonine-protein Kinase PLSTIRE, Crk2, MGC59692)
Gene Names
CDK6; PLSTIRE; MGC59692
Reactivity
Human, Mouse
Applications
IHC
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$465/0.05 mg | $2,090/5x0.05 mg
Immunohistochemistry (IHC)
(Peptide-+ Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using MEK1 (Ab-217) antibody.)
MEK1, Antibody (Cat# AAA856982)
Full Name
MEK1 (Ab-217) Antibody
Gene Names
MAP2K1; CFC3; MEK1; MKK1; MAPKK1; PRKMK1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$195/0.05 mg | $255/0.1 mg | $1,100/5x0.1 mg | $820/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells, treated with PMA (125ug/ml, 30mins), using CHOP (Phospho-Ser30) antibody.)
CHOP, Antibody (Cat# AAA858956)
Full Name
CHOP (Phospho-Ser30) Antibody
Gene Names
DDIT3; CHOP; CEBPZ; CHOP10; CHOP-10; GADD153; C/EBPzeta
Reactivity
Human, Mouse
Applications
WB, IHC, IF, EIA
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot Alpha-synuclein antibody at 2 /ml+293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 16 kDa Observed band size: 55 kDa Additional bands at: 118kDa. We are unsure as to the identity of these extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands)
Alpha-synuclein, Polyclonal Antibody (Cat# AAA719495)
Full Name
Rabbit anti-human Alpha-synuclein polyclonal Antibody, HRP conjugated
Gene Names
SNCA; PD1; NACP; PARK1; PARK4
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Sequence Information
Prothrombin Fragment 1+2, Recombinant Protein (Cat# AAA2029665)
Full Name
Recombinant Prothrombin Fragment 1+2 (F1+2)
Reactivity
Rat
Applications
May be suitable for use in other assays to be determined by the end user.
Purity
>95%
Pricing
$165/0.01 mg | $315/0.05 mg | $485/0.1 mg | $600/0.2 mg | $1,145/0.5 mg | $1,705/1 mg | $4,180/5 mg
Immunohistochemistry (IHC)
(P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using p44/42 MAP Kinase (phospho-Tyr204) antibody.)
p44/42 MAP Kinase, Antibody (Cat# AAA857485)
Full Name
p44/42 MAP Kinase (Phospho-Tyr204) Antibody
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$235/0.05 mg | $310/0.1 mg | $1,330/5x0.1 mg | $1,015/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of extracts from 293, Hela and HepG2 cells, using AurB/C (Ab-202/175) antibody.)
AurB/C, Polyclonal Antibody (Cat# AAA9410194)
Full Name
AurB/C (Ab-202/175) Antibody
Gene Names
AURKC; AIE2; AIK3; ARK3; AurC; SPGF5; STK13; HEL-S-90; aurora-C
Reactivity
Human
Applications
WB
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts from COLO205 cells, using PKC alpha (Phospho-Tyr657) antibody.)
PKC alpha, Antibody (Cat# AAA855654)
Full Name
PKC alpha (Phospho-Tyr657) Antibody
Gene Names
PRKCA; AAG6; PKCA; PRKACA; PKC-alpha
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Testing Data
(Peptide-+)
MYPT1, Antibody (Cat# AAA858577)
Full Name
MYPT1 (Ab-696) Antibody
Gene Names
PPP1R12A; MBS; M130; MYPT1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of extracts from cos7 cells untreated or treated with EGF using BAD(Phospho-Ser112) Antibody)
BAD, Polyclonal Antibody (Cat# AAA9405193)
Full Name
BAD (Phospho-Ser112) Antibody
Gene Names
Bad; Bbc2; AI325008
Reactivity
Human, Mouse
Applications
WB, IHC
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Immunohistochemistry (IHC)
(Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using PAK1/PAK2/PAK3 (Ab-423/402/421) antibody.)
PAK1/PAK2/PAK3, Antibody (Cat# AAA857362)
Full Name
PAK1/PAK2/PAK3 (Ab-423/402/421) Antibody
Gene Names
PAK1; PAKalpha
Reactivity
Human, Mouse, Rat
Applications
IHC
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$195/0.05 mg | $255/0.1 mg | $1,100/5x0.1 mg | $820/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Immunohistochemistry (IHC)
(Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using IRS-1 (Ab-636) antibody.)
IRS-1, Antibody (Cat# AAA856887)
Full Name
IRS-1(Ab-636) Antibody
Gene Names
IRS1; HIRS-1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$195/0.05 mg | $255/0.1 mg | $1,100/5x0.1 mg | $820/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot Vascular endothelial growth factor C Antibody at 2 /ml + 293T whole cell lysate at 20 SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 46 kDaObserved band size: 20 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Vascular endothelial growth factor C, Polyclonal Antibody (Cat# AAA715961)
Full Name
Rabbit anti-human Vascular endothelial growth factor C polyclonal Antibody, FITC
Gene Names
VEGFC; VRP; Flt4-L; LMPH1D
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells, using ALOX5 (Phospho-Ser523) Antibody.)
ALOX5, Antibody (Cat# AAA858202)
Full Name
ALOX5 (Phospho-Ser523) Antibody
Gene Names
ALOX5; 5-LO; 5LPG; LOG5; 5-LOX
Reactivity
Human, Rat
Applications
WB, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Testing Data
(Peptide - +Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Met (Ab-1003) Antibody (E021548).)
Met, Antibody (Cat# AAA857054)
Full Name
Met (Ab-1003) Antibody
Gene Names
MET; HGFR; AUTS9; RCCP2; c-Met; DFNB97
Reactivity
Human, Mouse, Rat
Applications
IHC
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$195/0.05 mg | $255/0.1 mg | $1,100/5x0.1 mg | $820/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Immunofluorescence (IF)
(P-peptide-+ Immunofluorescence analysis of HeLa cells, using Histone H3.3 (Phospho-Ser31) antibody.)
Histone H3.3, Antibody (Cat# AAA859330)
Full Name
Histone H3.3 (Phospho-Ser31) Antibody
Gene Names
H3F3A; H3F3; H3.3A
Reactivity
Human, Mouse, Rat
Applications
IF, EIA
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of extracts from HT-29 cells untreated or treated with hydroxyurea, using p53 (Ab-18) antibody and p53 (phospho-Thr18) antibody.)
p53, Antibody (Cat# AAA857506)
Full Name
p53 (Phospho-Thr18) Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human
Applications
WB
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$235/0.05 mg | $310/0.1 mg | $1,330/5x0.1 mg | $1,015/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Immunoblotting
(Alkali-stripped membranes from rFPR-expressing CHO cells (lane 11) and degranulated human neutrophils (lane 12) blotted with MBS190883)
FPR1, Monoclonal Antibody (Cat# AAA190883)
Full Name
FPR1 Monoclonal Antibody
Gene Names
FPR1; FPR; FMLP
Applications
IB, IF
Purity
Protein G purified
Pricing
$405/0.1 mg | $1,760/5x0.1 mg
SDS-Page
Matrix Metalloproteinase 8, Recombinant Protein (Cat# AAA2031518)
Full Name
Recombinant Matrix Metalloproteinase 8 (MMP8)
Gene Names
MMP8; HNC; CLG1; MMP-8; PMNL-CL
Applications
May be suitable for use in other assays to be determined by the end user.
Purity
> 90%
Pricing
$160/0.01 mg | $300/0.05 mg | $460/0.1 mg | $565/0.2 mg | $1,080/0.5 mg | $1,600/1 mg | $3,920/5 mg
