Rabbit anti-Human, Mouse CREB Polyclonal Antibody | anti-CREB1 antibody

CREB, phosphorylated (Ser121) (CBP, CREBBP, CREB binding protein, cAMP Response Element Binding Protein, RTS, RSTS, Rubinstein-Taybi syndrome)

Cat. Num. AAA631087

• Gene Names: CREB1; CREB; MGC9284
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: CREB; Polyclonal Antibody; phosphorylated (Ser121) (CBP; CREBBP; CREB binding protein; cAMP Response Element Binding Protein; RTS; RSTS; Rubinstein-Taybi syndrome); Anti -CREB; anti-CREB1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: This antibody is specific for the Ser-121-phosphorylated form of CREB. Species Crossreactivity: Reacts with human and mouse CREB. Other species have not been tested.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.

• Applicable Applications for anti-CREB1 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blot and Immunoprecipitation.
• Immunogen: A phospho-peptide spanning the Ser-121 phosphorylation site of human CREB.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

(Detection of phospho-CREB in HEK 293T cells using MBS631087 (top panel). Bottom panel: HEK 293T cells probled with CREB antibody. Lanes 2 and 4 (top and bottom): Samples treated with lambda-Phosphatase. Lanes 3 and 4 (top and bottom): Samples treated with 0.2mM H2O2.)

Related Product Information for anti-CREB1 antibody

Cyclic AMP Response Element Binding protein (CREB) is a basic / leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. CREB is also regulated by DNA-damaging stimuli and is directly phosphorylated on Ser-121 by the ataxia-telangiectasia-mutated protein kinase, ATM in response to ionizing radiation.

Product Categories/Family for anti-CREB1 antibody

Antibodies; Abs to Disease Markers

NCBI and Uniprot Product Information

• NCBI GI #: 33694240
• NCBI GeneID: 1385
• UniProt Accession #: Q6V963; P16220; Q16366; Q16367; Q4ZG78; Q53RU9; Q53X93; Q5U0J5
• Molecular Weight: 25,445 Da
• NCBI Official Full Name: CREB
• NCBI Official Synonym Full Names: cAMP responsive element binding protein 1
• NCBI Official Symbol: CREB1
• NCBI Official Synonym Symbols: CREB; MGC9284
• NCBI Protein Information: cyclic AMP-responsive element-binding protein 1; CREB-1; OTTHUMP00000163864; OTTHUMP00000163865; OTTHUMP00000206660; OTTHUMP00000206662; OTTHUMP00000206667; transactivator protein; active transcription factor CREB; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1
• UniProt Protein Name: CREB
• Protein Family: CREB-binding protein
• UniProt Entry Name: Q6V963_HUMAN

NCBI Description

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]

Uniprot Description

CREB: a transcription factor of the leucine zipper family of DNA binding proteins. Binds as a homodimer to the cAMP-responsive element (CRE). Phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Two splice-variant isoforms have been described.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 2q34

Cellular Component: nucleoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; enzyme binding; transcription cofactor activity; transcription factor activity

Biological Process: lactation; circadian rhythm; transcription from RNA polymerase II promoter; axon guidance; response to glucagon stimulus; viral reproduction; nerve growth factor receptor signaling pathway; positive regulation of osteoclast differentiation; positive regulation of transcription, DNA-dependent; stress-activated MAPK cascade; positive regulation of multicellular organism growth; toll-like receptor 3 signaling pathway; signal transduction; protein amino acid phosphorylation; toll-like receptor 10 signaling pathway; response to organic substance; toll-like receptor 5 signaling pathway; synaptic transmission; toll-like receptor 4 signaling pathway; epidermal growth factor receptor signaling pathway; response to drug; mitochondrion organization and biogenesis; Notch signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; protein stabilization; secretory granule organization and biogenesis; organelle organization and biogenesis; MyD88-independent toll-like receptor signaling pathway; positive regulation of hormone secretion; positive regulation of lipid biosynthetic process; memory; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; pituitary gland development; phospholipase C activation; toll-like receptor signaling pathway; positive regulation of fat cell differentiation; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; regulation of cell size

Disease: Histiocytoma, Angiomatoid Fibrous