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Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
10000 results for " phosphorylation" - showing 4350-4400
Western Blot (WB)
(Western blot analysis of phosphorylated recombinant human Tau protein, stimulated by GSK3β, using Rabbit Anti-Tau (Phospho-Ser409) Polyclonal Antibody Lane 1: Rabbit Anti-Tau (Phospho-Ser409) Polyclonal Antibody Lane 2: Rabbit Anti-Tau (Phospho-Ser409) Polyclonal Antibody pre-incubated with nonphoshpo-peptide Lane 3: Rabbit Anti-Tau (Phospho-Ser409) Polyclonal Antibody pre-incubated with phoshpo-peptide Secondary Antibody: Goat Anti-Rabbit IgG (H&L) [HRP] The signal was developed with LumiSensorTM HRP Substrate Kit)
Tau, Polyclonal Antibody (Cat# AAA627760)
Full Name
Tau, phosphorylated (Ser409) (Tau Protein, Microtubule-associated Protein, MAP)
Gene Names
tau; BcDNA:RE16764; CG12881; CG31057; CG5606; DmelCG31057; dtau
Reactivity
Human
Applications
EL/EIA, WB
Purity
Affinity Purified
Purified by Affinity chromatography.
Purified by Affinity chromatography.
Pricing
$420/0.04 mg | $1,885/5x0.04 mg
Western Blot (WB)
(Western blot analysis of the extracts from K562 cells using p73 (Ab-99) antibody and p73 (phospho-Tyr99) antibody.)
p73, Antibody (Cat# AAA857066)
Full Name
p73 (Ab-99) Antibody
Gene Names
TP73; P73
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$195/0.05 mg | $255/0.1 mg | $1,100/5x0.1 mg | $820/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of extract from 293 cells using IGF-1R(Phospho-Tyr1280) Antibody.)
IGF-1R, Polyclonal Antibody (Cat# AAA9409243)
Full Name
IGF-1R (Phospho-Tyr1280) Antibody
Gene Names
IGF1R; IGFR; CD221; IGFIR; JTK13
Reactivity
Human
Applications
WB
Purity
Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, HepG2 cells and COLO205 cells, using p27 Kip1 (Phospho-Thr198) antibody.)
p27 Kip1, Antibody (Cat# AAA859253)
Full Name
p27 Kip1 (Phospho-Thr198) Antibody
Gene Names
CDKN1B; KIP1; MEN4; CDKN4; MEN1B; P27KIP1
Reactivity
Human
Applications
WB, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of extracts from 3T3 cells, using Tuberin (Ab-981) antibody.)
Tuberin, Antibody (Cat# AAA852413)
Full Name
Tuberin (Ab-981) Antibody
Gene Names
TSC2; LAM; TSC4; PPP1R160
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Immunohistochemistry (IHC)
(P-peptide-+ Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using STAT2 (Phospho-Tyr690) antibody.)
STAT2, Antibody (Cat# AAA851660)
Full Name
STAT2 (Phospho-Tyr690) Antibody
Gene Names
STAT2; P113; IMD44; ISGF-3; STAT113
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot C-C motif chemokine 2 Antibody at 2 /ml + EC109 whole cell lysate at 20 SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 11kDaObserved band size: 50 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
C-C motif chemokine 2, Polyclonal Antibody (Cat# AAA715513)
Full Name
Rabbit anti-human C-C motif chemokine 2 polyclonal Antibody, FITC
Gene Names
CCL2; HC11; MCAF; MCP1; MCP-1; SCYA2; GDCF-2; SMC-CF; HSMCR30
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
VASP, Polyclonal Antibody (Cat# AAA835777)
Full Name
Phospho-VASP antibody
Applications
WB
Purity
Phospho-VASP antibody was purified by affinity chromatography
Pricing
$590/0.1 mg | $2,650/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells and HepG2 cells, using eNOS (Phospho-Thr494) antibody.)
eNOS, Antibody (Cat# AAA855807)
Full Name
eNOS (Phospho-Thr494) Antibody
Gene Names
NOS3; eNOS; ECNOS
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Immunohistochemistry (IHC)
(Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Myc (Ab-358) antibody.)
Myc, Antibody (Cat# AAA857495)
Full Name
Myc (Ab-358) Antibody
Gene Names
MYC; MRTL; MYCC; c-Myc; bHLHe39
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$195/0.05 mg | $255/0.1 mg | $1,100/5x0.1 mg | $820/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
GluR1a, Polyclonal Antibody (Cat# AAA620967)
Full Name
GluR1a (Glutamate Receptor), phosphorylated (Ser849)
Gene Names
gria1a; glur1a; gria1.1; ZfGluR1a; zfGRIA1[a]
Reactivity
Human, Mouse
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$550/0.1 mg | $2,455/5x0.1 mg
Western Blot (WB)
(Western Blot analysis of various cells with Phospho-Akt1/3 (Tyr437/434) Polyclonal Antibody at dilution of 1:1000)
Akt1/3, Polyclonal Antibody (Cat# AAA2535479)
Full Name
Phospho-Akt1/3 (Y437/434) Polyclonal Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Antigen Affinity Purified
Pricing
$130/0.02 mL | $395/0.2 mL | $250/0.12 mL | $170/0.06 mL | $1,715/5x0.2 mL
Western Blot (WB)
(Western blot analysis of the extracts from COLO cells using CDC2 (Ab-161) antibody.)
CDC2, Antibody (Cat# AAA857603)
Full Name
CDC2 (Ab-161) Antibody
Gene Names
CDK1; CDC2; CDC28A; P34CDC2
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$195/0.05 mg | $255/0.1 mg | $1,100/5x0.1 mg | $820/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Immunohistochemistry (IHC)
(P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human brain tissue using APC1 (Phospho-Ser355) antibody.)
APC1, Antibody (Cat# AAA851479)
Full Name
APC1 (Phospho-Ser355) Antibody
Gene Names
ANAPC1; APC1; MCPR; TSG24
Reactivity
Human, Mouse
Applications
IHC, EIA
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of extracts from 293 cells, using PKR (Ab-258) antibody.)
PKR, Antibody (Cat# AAA854455)
Full Name
PKR (Ab-258) Antibody
Gene Names
EIF2AK2; PKR; PRKR; EIF2AK1; PPP1R83
Reactivity
Human
Applications
WB, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Immunohistochemistry (IHC)
(Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using p70 S6 Kinase (phospho-Ser424) antibody.)
p70 S6 Kinase, Antibody (Cat# AAA857216)
Full Name
p70 S6 Kinase (Ab-424) Antibody
Gene Names
RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$195/0.05 mg | $255/0.1 mg | $1,100/5x0.1 mg | $820/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Testing Data
(P-peptide-+)
Coagulation Factor III, Antibody (Cat# AAA859742)
Full Name
Coagulation Factor III (Phospho-Ser290) Antibody
Gene Names
F3; TF; TFA; CD142
Reactivity
Human
Applications
WB, IHC, EIA
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western Blot showing STAT3 antibody used against full-length STAT3-His recombinant protein (1).)
STAT3, Monoclonal Antibody (Cat# AAA831137)
Full Name
STAT3 antibody
Gene Names
STAT3; APRF; HIES; ADMIO
Applications
EIA, WB
Pricing
$605/0.1 mL | $2,715/5x0.1 mL
Western Blot (WB)
(Western Blot analysis of COS7 cells using Phospho-Crk-L (Y207) Polyclonal Antibody)
Crk-L, Polyclonal Antibody (Cat# AAA9404326)
Full Name
Crk-L (Phospho-Tyr207) Polyclonal Antibody
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC, IF, EIA
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Western Blot (WB)
(Western Blot analysis of ectracts from HepG2 cells)
c-Met, Polyclonal Antibody (Cat# AAA626705)
Full Name
c-Met, phosphorylated (Tyr1234) (Hepatocyte Growth Factor Receptor, Scatter Factor Receptor, HGFR, SFR)
Gene Names
MET; c-Met
Reactivity
Human
Applications
EL/EIA, WB
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$550/0.1 mg | $2,455/5x0.1 mg
IkB alpha, Polyclonal Antibody (Cat# AAA625007)
Full Name
IkB alpha, phosphorylated (Ser32/Ser36) (NF-kappa-B Inhibitor alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, Major Histocompatibility Complex Enhancer-binding Protein MAD3, NFKBIA, IKBA, MAD3, NFKBI)
Gene Names
CHUK; IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha
Reactivity
Human, Mouse, Rat
Applications
IHC
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$465/0.05 mg | $2,090/5x0.05 mg
Testing Data
IRS-1, Antibody (Cat# AAA855659)
Full Name
IRS-1 (Phospho-Ser312) Antibody
Gene Names
IRS1; HIRS-1
Reactivity
Human, Mouse, Rat
Applications
WB, IF, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Immunohistochemistry (IHC)
(P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma using JunB (phospho-Ser259) antibody.)
JunB, Antibody (Cat# AAA857032)
Full Name
JunB (Phospho-Ser259) Antibody
Gene Names
JUNB; AP-1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$235/0.05 mg | $310/0.1 mg | $1,330/5x0.1 mg | $1,015/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Immunohistochemistry (IHC)
(P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using LIMK2 (phospho-Thr505) antibody.)
LIMK2, Antibody (Cat# AAA857469)
Full Name
LIMK2 (Phospho-Thr505) Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$235/0.05 mg | $310/0.1 mg | $1,330/5x0.1 mg | $1,015/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot
(Western Blot analysis using SYK antibodyWestern Blot showing SYK antibody used against truncated SYK-His recombinant protein (1) and PMA induced THP-1 cell lysate (2).)
SYK, Monoclonal Antibody (Cat# AAA830791)
Full Name
SYK antibody
Gene Names
Syk; Sykb
Applications
EIA, WB
Pricing
$675/0.1 mL | $3,020/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts from C6 cell untreated or treated with serum usingc-kit(phospho-Tyr936) Antibody.)
c-kit, Polyclonal Antibody (Cat# AAA9402830)
Full Name
c-kit (phospho-Tyr936) Antibody
Gene Names
KIT; PBT; SCFR; C-Kit; CD117
Reactivity
Human
Applications
IHC, WB
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant DCK protein (Right) or empty vector (Left) detected with DCK antibody)
DCK, Monoclonal Antibody (Cat# AAA532822)
Full Name
DCK antibody
Applications
FC/FACS, IF, WB
Purity
DCK antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Testing Data
B-Myb, Antibody (Cat# AAA858575)
Full Name
B-Myb (Ab-577/581) Antibody
Gene Names
MYBL2; BMYB; B-MYB
Reactivity
Human, Mouse, Rat
Applications
WB, IF, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Immunohistochemistry (IHC)
(P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human brain tissue using VEGFR1 (Phospho-Tyr1048) antibody.)
VEGFR1, Antibody (Cat# AAA854350)
Full Name
VEGFR1 (Phospho-Tyr1048) Antibody
Gene Names
FLT1; FLT; FLT-1; VEGFR1; VEGFR-1
Reactivity
Human, Mouse, Rat
Applications
IHC, EIA
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Testing Data
MEK1/2 (pS218/S222), Antibody (Cat# AAA462074)
Full Name
Rabbit anti MEK1/2 (pSer218/222), Phosphospecific
Reactivity
Human, Mouse, Rat, Chicken
Applications
ELISA, Western Blot
Purity
The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
Pricing
$310/0.1 mg | $1,380/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells, treated with EGF (200ng/ml, 5mins), using AKT1 Phospho-Thr308) antibody.)
AKT1, Antibody (Cat# AAA855182)
Full Name
AKT1 (Phospho-Thr308) Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot All lanes: Cytochrome b-c1 complex subunit 8 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 9.5 kDa Observed band size: 30kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Cytochrome b-c1 complex subunit 8, Polyclonal Antibody (Cat# AAA715270)
Full Name
Rabbit anti-human Cytochrome b-c1 complex subunit 8 polyclonal Antibody, HRP conjugated
Gene Names
UQCRQ; QPC; QCR8; QP-C; UQCR7; MC3DN4
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
K6PP, Polyclonal Antibody (Cat# AAA5302970)
Full Name
K6PP antibody
Applications
WB, EIA
Pricing
$405/0.1 mg | $1,805/5x0.1 mg
Testing Data
(P-peptide - +)
PAK3, Antibody (Cat# AAA851059)
Full Name
PAK3 (Phospho-Ser154) Antibody
Gene Names
PAK3; ARA; bPAK; MRX30; MRX47; OPHN3; PAK-3; PAK3beta; beta-PAK
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, treated with Adriamycin (0.5ug/ml, 24hours), using RFA2 (Phospho-Thr21) antibody.)
RFA2, Antibody (Cat# AAA851538)
Full Name
RFA2 (Phospho-Thr21) Antibody
Gene Names
RPA2; REPA2; RPA32; RP-A p32; RP-A p34
Reactivity
Human, Mouse
Applications
WB, IHC, EIA
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
RIPK1, Polyclonal Antibody (Cat# AAA540832)
Full Name
RIPK1 Antibody Affinity Purified
Gene Names
RIPK1; RIP; RIP1; RIP-1
Reactivity
Human
Applications
EIA, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Testing Data
(P-peptide-+)
AurB, Antibody (Cat# AAA859096)
Full Name
AurB (Phospho-Tyr12) Antibody
Gene Names
AURKB; AIK2; AIM1; ARK2; AurB; IPL1; STK5; AIM-1; ARK-2; STK-1; STK12; PPP1R48; aurkb-sv1; aurkb-sv2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of extracts from RAW264.7 cells, treated with UV (5mins), using MSK2 (Ab-568) antibody.)
MSK2, Antibody (Cat# AAA853073)
Full Name
MSK2 (Ab-568) Antibody
Gene Names
RPS6KA4; MSK2; RSK-B; S6K-alpha-4
Reactivity
Human, Mouse
Applications
WB, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Immunohistochemistry (IHC)
(P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT1 (phospho-Tyr701) antibody.)
STAT1, Antibody (Cat# AAA855342)
Full Name
STAT1 (Phospho-Tyr701) Antibody
Gene Names
STAT1; CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA, IP
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western Blot analysis of Jurkat cells with CD45 Polyclonal Antibody.)
CD45, Polyclonal Antibody (Cat# AAA2538847)
Full Name
CD45 Polyclonal Antibody
Gene Names
PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, EIA
Purity
Affinity purification
Pricing
$130/0.02 mL | $395/0.2 mL | $250/0.12 mL | $170/0.06 mL | $1,715/5x0.2 mL
SDS-Page
Aspartate Aminotransferase (AST), Recombinant Protein (Cat# AAA2010629)
Full Name
Recombinant Aspartate Aminotransferase (AST)
Gene Names
Got1; cCAT; Got-1; cAspAT; AI789014
Reactivity
Mus musculus (Mouse)
Applications
WB
Purity
> 97%
Pricing
$165/0.01 mg | $315/0.05 mg | $485/0.1 mg | $600/0.2 mg | $1,145/0.5 mg | $1,705/1 mg | $4,180/5 mg
Phosphorylation Extracellular Signal Regulated Kinase 1/2, ELISA Kit (Cat# AAA9774)
Full Name
Hamster Phosphorylation Extracellular Signal Regulated Kinase 1/2 ELISA Kit
Gene Names
BRI1; ATBRI1; BIN1; BR INSENSITIVE 1; BRASSINOSTEROID INSENSITIVE 1; BRI1; CABBAGE 2; CBB2; DWARF 2; DWF2; F23K16.30; F23K16_30
Reactivity
Hamster
Pricing
$540/48-Strip-Wells | $755/96-Strip-Wells | $3,410/5x96-Strip-Wells | $6,715/10x96-Strip-Wells
Western Blot (WB)
(Western blot analysis of extract from 3T3 cell, using FAK (phospho-Tyr397) antibody.)
FAK, Antibody (Cat# AAA857407)
Full Name
FAK (Phospho-Tyr397) Antibody
Gene Names
PTK2; FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$235/0.05 mg | $310/0.1 mg | $1,330/5x0.1 mg | $1,015/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Testing Data
(UV - +Western blot analysis of extracts from HT29 cells untreated or treated UV, using Chk1 (phospho-Ser280) antibody (Line 1 and 2).)
Chk1, Antibody (Cat# AAA851018)
Full Name
Chk1 (Phospho-Ser280) Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells treated with Hu using AIRE (Phospho-Ser156) Antibody.The lane on the right is treated with the antigen-specific peptide.)
AIRE, Polyclonal Antibody (Cat# AAA9401950)
Full Name
AIRE (Phospho-Ser156) Antibody
Gene Names
AIRE; APS1; APSI; PGA1; AIRE1; APECED
Reactivity
Human
Applications
WB
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts from NIH-3T3 cells, using p44/42 MAP Kinase (Ab-204) antibody and p44/42 MAP Kinase (phospho-Tyr204) antibody.)
p44/42 MAP Kinase, Antibody (Cat# AAA857205)
Full Name
p44/42 MAP Kinase (Ab-204) Antibody
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$195/0.05 mg | $255/0.1 mg | $1,100/5x0.1 mg | $820/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of extract from starved NIH/3T3 cells, using MARCKS (Ab-158) antibody and MARCKS (phospho-Ser158) antibody.)
MARCKS, Antibody (Cat# AAA856793)
Full Name
MARCKS (Phospho-Ser158) Antibody
Gene Names
MARCKS; MACS; 80K-L; PKCSL; PRKCSL
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$235/0.05 mg | $310/0.1 mg | $1,330/5x0.1 mg | $1,015/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of extracts from COS-7 cells, treated with forskolin (40nM, 30mins), using DARPP-32 (Ab-34) antibody.)
DARPP-32, Antibody (Cat# AAA852051)
Full Name
DARPP-32 (Ab-34) Antibody
Gene Names
PPP1R1B; DARPP32; DARPP-32
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Immunohistochemistry (IHC)
(P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p44/42 MAP Kinase (phospho-Thr202) antibody.)
p44/42 MAP Kinase, Antibody (Cat# AAA857416)
Full Name
p44/42 MAP Kinase (Phospho-Thr202) Antibody
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$235/0.05 mg | $310/0.1 mg | $1,330/5x0.1 mg | $1,015/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
MAP Kinase p44/42, Polyclonal Antibody (Cat# AAA628275)
Full Name
MAP Kinase p44/42, phosphorylated (Thr202/Tyr204) (MAPK3/MAPK1, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, ERT1, Extracellular Signal-regulated Kinase 2, ERK-2, MAP Kinase Isoform p42, p42-MAPK, Mitogen-activated Protein Kinase 2, MAP Kinas
Reactivity
Human, Mouse, Rat
Applications
EL/EIA, WB, IHC
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$465/0.05 mg | $2,090/5x0.05 mg
