Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells and HepG2 cells, using eNOS (Phospho-Thr494) antibody.)
eNOS Antibody | anti-eNOS antibody
eNOS (Phospho-Thr494) Antibody
Gene Names
NOS3; eNOS; ECNOS
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
eNOS; eNOS (Phospho-Thr494) Antibody; cNOS; Constitutive NOS; EC 1.14.13.39; EC-NOS; ECNOS; Endothelial NOS; Nitric-oxide synthase; endothelial; NOS; type III; NOS3; NOSIII; anti-eNOS antibody
Reactivity
Human, Mouse, Rat
Specificity
eNOS (Phospho-Thr494) antibody detects endogenous levels of eNOS only when phosphorylated at threonine 494.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
1203
Applicable Applications for anti-eNOS antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
ELISA: 1:5000
ELISA: 1:5000
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human eNOS around the phosphorylation site of threonine 494 (K-K-TP-F-K).
All Sites
Human: Thr494
Mouse: Thr494
Rat: Thr494
Mouse: Thr494
Rat: Thr494
Preparation and Storage
Store at-20 degree C for 1 year.
Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells and HepG2 cells, using eNOS (Phospho-Thr494) antibody.)
Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells and HepG2 cells, using eNOS (Phospho-Thr494) antibody.)
Product Categories/Family for anti-eNOS antibody
References
Janssens S.P., J. Biol. Chem. 267:14519-14522(1992). Janssens S.P., J. Biol. Chem. 267:22694-22694(1992). Marsden P.A., FEBS Lett. 307:287-293(1992).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
67,862 Da
NCBI Official Full Name
nitric oxide synthase, endothelial isoform 1
NCBI Official Synonym Full Names
nitric oxide synthase 3
NCBI Official Symbol
NOS3
NCBI Official Synonym Symbols
eNOS; ECNOS
NCBI Protein Information
nitric oxide synthase, endothelial
UniProt Protein Name
Nitric oxide synthase, endothelial
UniProt Gene Name
NOS3
UniProt Synonym Gene Names
cNOS; eNOS; NOSIII
NCBI Description
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016]
Uniprot Description
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Research Articles on eNOS
Similar Products
Product Notes
The eNOS nos3 (Catalog #AAA855807) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The eNOS (Phospho-Thr494) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's eNOS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500~1:1000 ELISA: 1:5000. Researchers should empirically determine the suitability of the eNOS nos3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "eNOS, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
