Aspartate Aminotransferase (AST) Recombinant Protein | AST recombinant protein

Recombinant Aspartate Aminotransferase (AST)

Cat. Num. AAA2010629

• Gene Names: Got1; cCAT; Got-1; cAspAT; AI789014
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Aspartate Aminotransferase (AST); Recombinant Aspartate Aminotransferase (AST); AST recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGS-MAPPSVFAQV PQAPPVLVFK LTADFRDDPD PRKVNLGVGA YRTDESQPWV LPVVRKVEQK IANDNSLNHE YLPILGLAEF RSCASRLVLG DNSLAIRENR VGGVQSLGGT GALRIGADFL GRWYNGTDNK NTPIYVSSPT WENHNAVFSA AGFKDIRPYC YWDAEKRGLD LQGFLNDLEN APEFSIFVLH ACAHNPTGTD PTPEQWKQIA AVMQRRFLFP FFDSAYQGFA SGDLEKDAWA IRYFVSEGFE LFCAQSFSKN FGLYNERVGN LTVVGKESDS VLRVLSQMEK IVRITWSNPP AQGARIVAAT LSDPELFKEW KGNVKTMADR ILTMRSELRA RLEALKTPGT WSHITEQIGM FSFTGLNPKQ VEYLVNEKHI YLLPSGRINM CGLTTKNLDY VATSIHEAVT KIQ
• Sequence Length: 413

• Applicable Applications for AST recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Met1~Gln413 (Accession # P05201) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 160298209
• NCBI GeneID: 14718
• NCBI Accession #: NP_034454.2
• NCBI GenBank Nucleotide #: NM_010324.2
• UniProt Accession #: P05201; Q3UJH8
• Molecular Weight: 47.5kDa
• NCBI Official Full Name: aspartate aminotransferase, cytoplasmic
• NCBI Official Synonym Full Names: glutamate oxaloacetate transaminase 1, soluble
• NCBI Official Symbol: Got1
• NCBI Official Synonym Symbols: cCAT; Got-1; cAspAT; AI789014
• NCBI Protein Information: aspartate aminotransferase, cytoplasmic; transaminase A; cysteine transaminase, cytoplasmic; cytosolic aspartate aminotransferase; cysteine aminotransferase, cytoplasmic
• UniProt Protein Name: Aspartate aminotransferase, cytoplasmic
• Protein Family: Allatostatin
• UniProt Gene Name: Got1
• UniProt Synonym Gene Names: cAspAT; cCAT
• UniProt Entry Name: AATC_MOUSE

Uniprot Description

GOT1: Plays a key role in amino acid metabolism. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Protein type: EC 2.6.1.1; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - alanine, aspartate and glutamate; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - cysteine and methionine; Transferase; EC 2.6.1.3; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis

Cellular Component: lysosome; cytoplasm; nerve terminal; nucleus; cytosol

Molecular Function: transferase activity; cysteine transaminase activity; phosphatidylserine decarboxylase activity; carboxylic acid binding; aspartate transaminase activity; transaminase activity; catalytic activity; pyridoxal phosphate binding

Biological Process: amino acid metabolic process; dicarboxylic acid metabolic process; oxaloacetate metabolic process; glycerol biosynthetic process; glutamate catabolic process to aspartate; fatty acid homeostasis; aspartate biosynthetic process; biosynthetic process; aspartate metabolic process; aspartate catabolic process; glutamate catabolic process to 2-oxoglutarate; amino acid biosynthetic process