HER2 / ErbB2 recombinant protein

Recombinant Mouse HER2 / ErbB2 Protein (Fc tag)

Cat. Num. AAA2546589

• Gene Names: Erbb2; Neu; HER2; HER-2; c-neu; Erbb-2; c-erbB2; l11Jus8; mKIAA3023
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: HER2 / ErbB2; Recombinant Mouse HER2 / ErbB2 Protein (Fc tag); RP23-355L10.8; Erbb-2; HER-2; HER2; Neu; c-erbB2; c-neu; mKIAA3023; p185erbB2; HER2 / ErbB2 recombinant protein

• Host: Human Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 1256

• Application Notes: The secreted recombinant mouse ERBB2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 872 amino acids and has a calculated molecular mass of 96.7 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 120-130 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Thr 23
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for HER2 / ErbB2 recombinant protein

Background: Human epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein, and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on.

Description: A DNA sequence encoding the mouse ERBB2 (P70424) extracellular domain (Met 1-Thr 653) was fused with the Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 54873610
• NCBI GeneID: 13866
• NCBI Accession #: NP_001003817.1
• NCBI GenBank Nucleotide #: NM_001003817.1
• UniProt Accession #: P70424; Q61525; Q6ZPE0
• Molecular Weight: 138,579 Da
• NCBI Official Full Name: receptor tyrosine-protein kinase erbB-2
• NCBI Official Synonym Full Names: erb-b2 receptor tyrosine kinase 2
• NCBI Official Symbol: Erbb2
• NCBI Official Synonym Symbols: Neu; HER2; HER-2; c-neu; Erbb-2; c-erbB2; l11Jus8; mKIAA3023
• NCBI Protein Information: receptor tyrosine-protein kinase erbB-2
• UniProt Protein Name: Receptor tyrosine-protein kinase erbB-2
• UniProt Gene Name: Erbb2
• UniProt Synonym Gene Names: Kiaa3023; Neu

Uniprot Description

HER2: a proto-oncogenic receptor tyrosine kinase of the EGFR family. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. Not activated by EGF, TGF- alpha and amphiregulin. Amplified in breast cancer. Overexpression induces constitutive activity, and the gene is amplified or overexpressed in up to 30% of breast cancers, correlating with poor survival. The antibody Herceptin is approved for treatment of metastatic breast cancer with HER2 amplification/overexpression. Somatic mutations seen in 4% of lung cancers and also in breast, gastric, ovarian cancer and glioblastoma. One SNP shows predisposition to breast and gastric cancer. Inhibitors: Herceptin, lapatinib, PKI-166, EKB-569, CI-1033.

Protein type: EC 2.7.10.1; EGFR family; Kinase, protein; Membrane protein, integral; Oncoprotein; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Chromosomal Location of Human Ortholog: 11 61.75 cM|11 D

Cellular Component: apical plasma membrane; basal plasma membrane; basolateral plasma membrane; cytoplasm; cytoplasmic vesicle; endosome membrane; lateral loop; lipid raft; microvillus; myelin sheath; nucleus; perinuclear region of cytoplasm; plasma membrane; postsynaptic membrane; receptor complex

Molecular Function: glycoprotein binding; growth factor binding; Hsp90 protein binding; identical protein binding; protein binding; protein C-terminus binding; protein heterodimerization activity; protein phosphatase binding; protein-tyrosine kinase activity; transmembrane receptor activity; transmembrane receptor protein tyrosine kinase activity; ubiquitin protein ligase binding

Biological Process: cell surface receptor linked signal transduction; glial cell differentiation; heart development; motor axon guidance; myelination; negative regulation of apoptosis; negative regulation of immature T cell proliferation in the thymus; nervous system development; neuromuscular junction development; oligodendrocyte differentiation; peripheral nervous system development; phosphoinositide 3-kinase cascade; positive regulation of cell adhesion; positive regulation of cell growth; positive regulation of cell proliferation; positive regulation of epithelial cell proliferation; positive regulation of GTPase activity; positive regulation of MAP kinase activity; positive regulation of MAPKKK cascade; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein amino acid phosphorylation; positive regulation of Ras protein signal transduction; positive regulation of transcription from RNA polymerase I promoter; positive regulation of transcription from RNA polymerase III promoter; positive regulation of translation; protein amino acid autophosphorylation; regulation of microtubule-based process; response to axon injury; signal transduction; sympathetic nervous system development; transmembrane receptor protein tyrosine kinase signaling pathway; wound healing