Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
10000 results for " phosphorylation" - showing 4000-4050
SDS-Page
Noggin (NOG), Recombinant Protein (Cat# AAA2011409)
Full Name
Recombinant Noggin (NOG)
Applications
WB, EIA, IP
Purity
>98%
Pricing
$160/0.01 mg | $290/0.05 mg | $450/0.1 mg | $545/0.2 mg | $1,055/0.5 mg | $1,550/1 mg | $3,800/5 mg
Sequence Information
Lymphotoxin Beta (LTb), Recombinant Protein (Cat# AAA2012386)
Full Name
Recombinant Lymphotoxin Beta (LTb)
Gene Names
Ltb; p33; Tnfc; LTbeta; Tnfsf3; AI662801
Reactivity
Mouse
Applications
May be suitable for use in other assays to be determined by the end user.
Purity
>98%
Pricing
$130/0.01 mg | $225/0.05 mg | $315/0.1 mg | $390/0.2 mg | $735/0.5 mg | $1,080/1 mg | $2,625/5 mg | $11,805/5x5 mg
Western Blot (WB)
(Western blot analysis of extract from 293 cell untreated or treated with PMA, using Rac1 (phospho-Ser71) antibody.)
Rac1, Antibody (Cat# AAA856763)
Full Name
Rac1 (Phospho-Ser71) Antibody
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$235/0.05 mg | $310/0.1 mg | $1,330/5x0.1 mg | $1,015/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Immunohistochemistry (IHC)
(P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human brain tissue using LIMK2 (Phospho-Ser283) antibody.)
LIMK2, Antibody (Cat# AAA853253)
Full Name
LIMK2 (Phospho-Ser283) Antibody
Reactivity
Human, Mouse, Rat
Applications
IHC, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
RAF-1, Polyclonal Antibody (Cat# AAA221524)
Full Name
RABBIT ANTI RAF-1 (pSer642)
Reactivity
Bovine (Expected from Sequence), Chicken (Expected from Sequence), Dog (Expected from Sequence), Human (Expected from Sequence), Mouse (Expected from Sequence), Primate (Expected from Sequence)
Applications
WB
Pricing
$625/0.1 mL | $2,670/5x0.1 mL
SDS-Page
DNA Repair Protein RAD50 (RAD50), Recombinant Protein (Cat# AAA2009740)
Full Name
Recombinant DNA Repair Protein RAD50 (RAD50)
Gene Names
RAD50; NBSLD; RAD502; hRad50
Applications
May be suitable for use in other assays to be determined by the end user.
Purity
> 97%
Pricing
$185/0.01 mg | $355/0.05 mg | $540/0.1 mg | $665/0.2 mg | $1,285/0.5 mg | $1,915/1 mg | $4,695/5 mg
Testing Data
(Peptide-+Western blot analysis of extracts from HeLa cells, using Glycogen Synthase (Phospho-Ser641) Antibody.)
Glycogen Synthase, Antibody (Cat# AAA853351)
Full Name
Glycogen Synthase (Phospho-Ser641) Antibody
Gene Names
GYS1; GSY; GYS
Reactivity
Human, Mouse
Applications
WB, EIA
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Testing Data
Kv2.1, Antibody (Cat# AAA852410)
Full Name
Kv2.1 (Phospho-Ser805) Antibody
Gene Names
KCNB1; DRK1; Kv2.1
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, treated with TNF-a (20ng/ml, 2mins), using CD136/RON (Phospho-Ser1394) antibody.)
CD136/RON, Antibody (Cat# AAA859051)
Full Name
CD136/RON (Phospho-Ser1394) Antibody
Gene Names
MST1R; RON; PTK8; CD136; NPCA3; CDw136
Reactivity
Human, Mouse
Applications
WB, EIA
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
IKBE, Polyclonal Antibody (Cat# AAA626438)
Full Name
IKBE (NFKBIE, NF-kappa-B Inhibitor epsilon, NF-kappa-BIE, I-kappa-B-epsilon, IkB-E, IkB-epsilon, IkappaBepsilon)
Gene Names
NFKBIE; IKBE
Reactivity
Human, Mouse
Applications
WB, IHC
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$465/0.05 mg | $2,090/5x0.05 mg
Immunohistochemistry (IHC)
(Peptide-+ Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using LKB1 (Ab-334) antibody.)
LKB1, Antibody (Cat# AAA856930)
Full Name
LKB1 (Ab-334) Antibody
Gene Names
STK11; PJS; LKB1; hLKB1
Reactivity
Human, Mouse
Applications
WB, IHC, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$195/0.05 mg | $255/0.1 mg | $1,100/5x0.1 mg | $820/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of extracts from NIH/3T3 cells, untreated or PDGF-stimulated for the indicated times, using Phospho-PLCgamma1 (Tyr783) Antibody (upper) or PLCgamma1 P4074-42N (lower).)
Phospholipase C, gamma 1, Polyclonal Antibody (Cat# AAA630460)
Full Name
Phospholipase C, gamma 1, phosphorylated (Tyr783) (PLCg1)
Gene Names
PLCG1; PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1
Reactivity
Human, Mouse, Rat
Applications
EL/EIA, WB, IP, IHC
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Pricing
$660/0.1 mL | $2,955/5x0.1 mL
Sequence Information
TAK1, Recombinant Protein (Cat# AAA2029813)
Full Name
Recombinant TAK1 Like Protein (TAK1L)
Applications
WB
Purity
> 97%
Pricing
$160/0.01 mg | $295/0.05 mg | $460/0.1 mg | $560/0.2 mg | $1,075/0.5 mg | $1,595/1 mg | $3,905/5 mg
Western Blot (WB)
(Western blot All lanes: Cytochrome b-c1 complex subunit 10 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 6.4kDa Observed band size: 80 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands)
Cytochrome b-c1 complex subunit 10, Polyclonal Antibody (Cat# AAA715423)
Full Name
Rabbit anti- human Cytochrome b-c1 complex subunit 10 polyclonal Antibody, FITC
Gene Names
UQCR11; UQCR; QCR10; 0710008D09Rik
Reactivity
Human
Applications
EIA
Purity
>95%, Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
SDS-Page
Nuclear Receptor Coactivator 3, Recombinant Protein (Cat# AAA2031278)
Full Name
Recombinant Nuclear Receptor Coactivator 3 (NCOA3)
Gene Names
Ncoa3; Actr; Aib1; Rac3; Src3; pCip; Tram1; p/Cip; KAT13B; Tram-1; bHLHe42; AW321064; 2010305B15Rik
Reactivity
Human
Applications
May be suitable for use in other assays to be determined by the end user.
Purity
> 97%
Pricing
$160/0.01 mg | $290/0.05 mg | $450/0.1 mg | $545/0.2 mg | $1,055/0.5 mg | $1,550/1 mg | $3,800/5 mg
Immunohistochemistry (IHC)
(Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SAPK/JNK (Ab-183) Antibody.)
SAPK/JNK, Antibody (Cat# AAA857669)
Full Name
SAPK/JNK (Ab-183) Antibody
Gene Names
MAPK9; JNK2; SAPK; p54a; JNK2A; JNK2B; PRKM9; JNK-55; SAPK1a; JNK2BETA; p54aSAPK; JNK2ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$195/0.05 mg | $255/0.1 mg | $1,100/5x0.1 mg | $820/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot All lanes: 60S ribosomal protein L15 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 22.4kDa Observed band size: 70 kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
60S ribosomal protein L15, Polyclonal Antibody (Cat# AAA715886)
Full Name
Rabbit anti-human 60S ribosomal protein L15 polyclonal Antibody, Biotin conjugated
Gene Names
RPL15; L15; EC45; DBA12; RPL10; RPLY10; RPYL10
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Immunohistochemistry (IHC)
(P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human brain tissue using MDC1 (Phospho-Ser513) antibody.)
MDC1, Antibody (Cat# AAA853903)
Full Name
MDC1 (Phospho-Ser513) Antibody
Gene Names
MDC1; NFBD1
Reactivity
Human
Applications
IHC, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of extracts from 3T3 cells, using iNOS (Ab-151) antibody.)
iNOS, Antibody (Cat# AAA855710)
Full Name
iNOS (Ab-151) Antibody
Gene Names
NOS2; NOS; INOS; NOS2A; HEP-NOS
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western Blot analysis of 293T cells using Phospho-p120 (Y228) Polyclonal Antibody)
p120, Polyclonal Antibody (Cat# AAA9412244)
Full Name
p120 (Phospho-Tyr228) Polyclonal Antibody
Gene Names
CTNND1; CAS; p120; CTNND; P120CAS; P120CTN; p120(CAS); p120(CTN)
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, EIA
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
ERK/MAPK, Polyclonal Antibody (Cat# AAA221791)
Full Name
RABBIT ANTI ERK/MAPK (pThr202/pTyr204)
Gene Names
Mapk3; p44; ERK1; ERT2; MNK1; Erk-1; Esrk1; MAPK1; Prkm3; p44erk1; p44mapk
Reactivity
Bovine (Expected from Sequence), Chicken (Expected from Sequence), Dog (Expected from Sequence), Human, Monkey (Expected from Sequence), Mouse (Expected from Sequence), Xenopus (Expected from Sequence), Zebrafish (Expected from Sequence)
Applications
WB
Pricing
$625/0.1 mL | $2,670/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts from 3T3 cells, treated with starved (24hours), using VCP (Phospho-Ser352) antibody.)
VCP, Antibody (Cat# AAA852196)
Full Name
VCP (Phospho-Ser352) Antibody
Gene Names
VCP; p97; TERA; CDC48
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot All lanes: Charged multivesicular body protein 2a antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 24 kDa Observed band size: 16kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Charged multivesicular body protein 2a, Polyclonal Antibody (Cat# AAA715778)
Full Name
Rabbit anti-human Charged multivesicular body protein 2a polyclonal Antibody, FITC
Gene Names
CHMP2A; BC2; BC-2; VPS2; CHMP2; VPS2A
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, treated with Adriamycin (0.5ug/ml, 24hours), using MNK1 (Phospho-Thr255) antibody.)
MNK1, Antibody (Cat# AAA852933)
Full Name
MNK1 (Phospho-Thr255) Antibody
Gene Names
MKNK1; MNK1
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Testing Data
(P-peptide - +Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using LIMK1/2 (Phospho-Thr508/505) Antibody.)
LIMK1/2, Antibody (Cat# AAA854176)
Full Name
LIMK1/2 (Phospho-Thr508/505) Antibody
Gene Names
LIMK1; LIMK; LIMK-1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells, treated with PMA (125ng/ml, 30mins), using TAL-1 (Phospho-Ser122) antibody.)
TAL-1, Antibody (Cat# AAA853777)
Full Name
TAL-1 (Phospho-Ser122) Antibody
Gene Names
TAL1; SCL; TCL5; tal-1; bHLHa17
Reactivity
Human, Rat
Applications
WB, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Immunohistochemistry (IHC)
(P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p53 (phospho-Ser6) antibody.)
p53, Antibody (Cat# AAA857627)
Full Name
p53 (Phospho-Ser6) Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human, Mouse
Applications
WB, IHC
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$235/0.05 mg | $310/0.1 mg | $1,330/5x0.1 mg | $1,015/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Immunohistochemistry (IHC)
(P-Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using SHP-1 (Phospho-Tyr536) Antibody.)
SHP-1, Antibody (Cat# AAA857273)
Full Name
SHP-1 (Phospho-Tyr536) Antibody
Gene Names
PTPN6; HCP; HCPH; SHP1; SHP-1; HPTP1C; PTP-1C; SHP-1L; SH-PTP1
Reactivity
Human, Mouse, Rat
Applications
IHC
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$235/0.05 mg | $310/0.1 mg | $1,330/5x0.1 mg | $1,015/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot All lanes: HCLS1-associated protein X-1 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 31 kDa Observed band size: 40kDa Additional bands at: 90 kDa.We are unsure as to the identity of this extra band. Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
HCLS1-associated protein X-1, Polyclonal Antibody (Cat# AAA715815)
Full Name
Rabbit anti-human HCLS1-associated protein X-1 polyclonal Antibody, Biotin conjugated
Gene Names
HAX1; SCN3; HS1BP1; HCLSBP1
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Immunohistochemistry
(Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using IGF1R (Phospho-Tyr980) Antibody..)
IGF1R, Antibody (Cat# AAA856795)
Full Name
IGF1R (Phospho-Tyr980) Antibody
Gene Names
IGF1R; IGFR; CD221; IGFIR; JTK13
Reactivity
Human, Mouse, Rat
Applications
IHC
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$235/0.05 mg | $310/0.1 mg | $1,330/5x0.1 mg | $1,015/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of extract from HeLa cells, untreated or treated with PMA (200nM, 30min), using p90RSK (Ab-352) antibody.)
p90RSK, Antibody (Cat# AAA857377)
Full Name
p90RSK (Ab-352) Antibody
Gene Names
RPS6KA1; RSK; HU-1; RSK1; p90Rsk; MAPKAPK1A
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$195/0.05 mg | $255/0.1 mg | $1,100/5x0.1 mg | $820/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western Blot showing CDC2 antibody used against Jurkat (1) cell lysate.)
Cdc2, Monoclonal Antibody (Cat# AAA831971)
Full Name
Cdc2 antibody
Gene Names
cdc2; 5363; cdc; Cdc2; CDC2; cdc2Dm; CDCDm; Cdk-1; cdk1; Cdk1; CDK1; CDK1/CDC2; CG5363; Dcdc2; dCdk1; Dm cdc2; Dmcdc2; DmCdc2; DmCdk1; DmelCG5363; group 4; l(2)31Eh
Applications
EIA, FC/FACS, WB
Pricing
$605/0.1 mL | $2,715/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts from mouse brain tissue using Tau (Ab-262) Antibody and Tau (Phospho-Ser262) Antibody.)
Tau, Antibody (Cat# AAA858556)
Full Name
Tau (Phospho-Ser262) Antibody
Gene Names
MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Immunohistochemistry (IHC)
(Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using FOXO3A (Ab-253) antibody.)
FOXO3A, Antibody (Cat# AAA857276)
Full Name
FOXO3A (Ab-253) Antibody
Gene Names
FOXO3; FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$195/0.05 mg | $255/0.1 mg | $1,100/5x0.1 mg | $820/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
ZAP-70-PE, Monoclonal Antibody (Cat# AAA670249)
Full Name
Mouse Anti-Human ZAP-70-PE
Reactivity
Human
Applications
ELISA, Flow Cytometry
Pricing
$195/25 mg | $720/5x25 mg
Western Blot (WB)
(Western Blot analysis of 3T3 cells using Phospho-MRE11 (S264) Polyclonal Antibody)
MRE11, Polyclonal Antibody (Cat# AAA9401155)
Full Name
MRE11 (Phospho-Ser264) Polyclonal Antibody
Gene Names
MRE11A; ATLD; HNGS1; MRE11; MRE11B
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Dok1, Polyclonal Antibody (Cat# AAA626478)
Full Name
Dok1, phosphorylated (Tyr362) (Docking Protein 1, Downstream Of Tyrosine Kinase 1, MGC117395, MGC138860, p62(dok), p62dok, P62DOK, pp62)
Gene Names
DOK1; P62DOK; MGC117395; MGC138860
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$465/0.05 mg | $2,090/5x0.05 mg
Western blot
(Western blot analysis of extracts from COS cells, treated with EGF (200ng/ml, 30mins), using CD18/ITGB2 (Phospho-Thr758) antibody.)
CD18/ITGB2, Antibody (Cat# AAA858983)
Full Name
CD18/ITGB2 (Phospho-Thr758) Antibody
Gene Names
ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
Reactivity
Human, Mouse, Rat
Applications
WB, IF, EIA
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
AKT (Y326), Blocking Peptide (Cat# AAA9224987)
Full Name
Phospho-AKT(Y326) Antibody Blocking peptide
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant CDK2 protein (Right) or empty vector (Left) detected with CDK2 antibody)
CDK2, Monoclonal Antibody (Cat# AAA533407)
Full Name
CDK2 antibody
Gene Names
CDK2; CDKN2; p33(CDK2)
Applications
FC/FACS, IF, WB
Purity
CDK2 antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Gene Sequencing (extract)
ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a), Recombinant Protein (Cat# AAA2011058)
Full Name
Recombinant ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a)
Gene Names
ATP4A; ATP6A
Reactivity
Homo sapiens (Human)
Applications
WB
Purity
> 95%
Pricing
$150/0.01 mg | $265/0.05 mg | $395/0.1 mg | $480/0.2 mg | $920/0.5 mg | $1,355/1 mg | $3,315/5 mg | $14,910/5x5 mg
Western Blot (WB)
(Western blot analysis of extracts from NIH/3T3 and 293 cells, untreated or UV-treated, and C6 cells, untreated or anisomycin-treated, using MBS633592.)
MAP Kinase p38, Monoclonal Antibody (Cat# AAA633592)
Full Name
MAP Kinase p38, phosphorylated (Thr180, Ty182) (Mitogen Activated Protein Kinase p38, MAPK p38)
Reactivity
Human, Mouse, Rat
Applications
WB, IP, FC/FACS
Purity
Ascites
Ascites
Ascites
Pricing
$660/0.1 mL | $2,955/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells, using JAK3 (Phospho-Tyr785) antibody.)
JAK3, Antibody (Cat# AAA858964)
Full Name
JAK3 (Phospho-Tyr785) Antibody
Gene Names
JAK3; JAKL; LJAK; JAK-3; L-JAK; JAK3_HUMAN
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
IKK alpha, Polyclonal Antibody (Cat# AAA626752)
Full Name
IKK alpha, phosphorylated (Thr23) Inhibitor Of Nuclear Factor kappa-B Kinase alpha Subunit, I kappa-B Kinase alpha, IkBKA, IKK-A, IkappaB Kinase, I-kappa-B Kinase 1, IKK1, Conserved Helix-loop-Helix Ubiquitous Kinase, Nuclear Factor NFkappaB Inhibitor Kin
Gene Names
CHUK; IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
$465/0.05 mg | $2,090/5x0.05 mg
Western Blot (WB)
(Western blot analysis of extracts from 293 cells, using Bcl-6 (Ab-333) antibody.)
Bcl-6, Antibody (Cat# AAA855734)
Full Name
Bcl-6 (Ab-333) Antibody
Gene Names
BCL6; BCL5; LAZ3; BCL6A; ZNF51; ZBTB27
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma tissue using CHK2 (Ab-387) Antibody.)
CHK2, Antibody (Cat# AAA857347)
Full Name
CHK2 (Ab-387) Antibody
Gene Names
CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$195/0.05 mg | $255/0.1 mg | $1,100/5x0.1 mg | $820/5x0.05 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot (WB)
(Western blot All lanes: Phosphatidylserine synthase 1 antibody at at 2 /mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysate SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size: 52 kDa Observed band size: 36kDa Why is the actual band size different from the predicted Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include... · post-translational modification - e.g. phosphorylation, glycosylation etc which increases the size of the protein · post-translation cleavage - e.g. many proteins are synthesized as pro-proteins, and then cleaved to give the active form, e.g. pro-caspases · splice variants - alternative splicing may create different sized proteins from the same gene · relative charge - the composition of amino acids (charged vs non-charged) multimers - e.g. dimerisation of a protein. This is usually prevented in reducing conditions, although strong interactions can result in the appearance of higher bands.)
Phosphatidylserine synthase 1, Polyclonal Antibody (Cat# AAA715976)
Full Name
Rabbit anti-human Phosphatidylserine synthase 1 polyclonal Antibody, Biotin conjugated
Gene Names
PTDSS1; LMHD; PSS1; PSSA
Reactivity
Human
Applications
EIA
Purity
Caprylic Acid Ammonium Sulfate Precipitation
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot analysis of 293T MOUSE-BRAIN using p-14-3-3 theta/tau (S232) antibody.)
14-3-3 theta/tau, Polyclonal Antibody (Cat# AAA9412129)
Full Name
14-3-3 theta/tau (Phospho-Ser232) Polyclonal Antibody
Gene Names
YWHAQ; 1C5; HS1; 14-3-3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, EIA
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$245/0.05 mL | $305/0.1 mL | $1,230/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts from 293 cells treated with EGF (200ng/ml, 5mins), using Ephrin B (Ab-330) antibody.)
EFNB1/2, Antibody (Cat# AAA859936)
Full Name
EFNB1/2 (Ab-330) Antibody
Gene Names
EFNB2; HTKL; EPLG5; Htk-L; LERK5
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $465/0.1 mL (Biotin) | $465/0.1 mL (FITC) | $465/0.1 mL (AF350) | $465/0.1 mL (AF405) | $465/0.1 mL (AF488) | $465/0.1 mL (AF555) | $465/0.1 mL (AF568)
Western Blot
(Western Blot analysis using BTRC antibodyWestern Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant BTRC protein (Right) or empty vector (Left) detected with BTRC antibody)
BTRC, Monoclonal Antibody (Cat# AAA533939)
Full Name
BTRC antibody
Gene Names
BTRC; FWD1; FBW1A; FBXW1; bTrCP; FBXW1A; bTrCP1; betaTrCP; BETA-TRCP
Applications
WB
Purity
BTRC antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
