Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, treated with Adriamycin (0.5ug/ml, 24hours), using MNK1 (Phospho-Thr255) antibody.)
MNK1 Antibody | anti-MNK1 antibody
MNK1 (Phospho-Thr255) Antibody
Gene Names
MKNK1; MNK1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
MNK1; MNK1 (Phospho-Thr255) Antibody; EC 2.7.11.1; kinase Mnk1; Map kinase interacting kinase; MAP kinase signal-integrating kinase 1; MAP kinase-interacting serine/threonine kinase 1; MKNK1; anti-MNK1 antibody
Reactivity
Human, Mouse, Rat
Specificity
MNK1 (Phospho-Thr255) antibody detects endogenous levels of MNK1 only when phosphorylated at threonine 255.
Purity/Purification
Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
424
Applicable Applications for anti-MNK1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
ELISA: 1:10000
ELISA: 1:10000
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human MNK1 around the phosphorylation site of threonine 255 (L-T-TP-P-C).
All Sites
Human: Thr255
Mouse: Thr214
Rat: Thr202
Mouse: Thr214
Rat: Thr202
Preparation and Storage
Store at-20 degree C for 1 year.
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, treated with Adriamycin (0.5ug/ml, 24hours), using MNK1 (Phospho-Thr255) antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HeLa cells, treated with Adriamycin (0.5ug/ml, 24hours), using MNK1 (Phospho-Thr255) antibody.)
Product Categories/Family for anti-MNK1 antibody
References
Fukunaga R., EMBO J. 16:1921-1933(1997). Knauf U., Mol. Cell. Biol. 21:5500-5511(2001). O'Loghlen A., Exp. Cell Res. 299:343-355(2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,104 Da
NCBI Official Full Name
MAP kinase-interacting serine/threonine-protein kinase 1 isoform 3
NCBI Official Synonym Full Names
MAP kinase interacting serine/threonine kinase 1
NCBI Official Symbol
MKNK1
NCBI Official Synonym Symbols
MNK1
NCBI Protein Information
MAP kinase-interacting serine/threonine-protein kinase 1
UniProt Protein Name
MAP kinase-interacting serine/threonine-protein kinase 1
UniProt Gene Name
MKNK1
UniProt Synonym Gene Names
MNK1; MAPK signal-integrating kinase 1; Mnk1
NCBI Description
This gene encodes a Ser/Thr protein kinase that interacts with, and is activated by ERK1 and p38 mitogen-activated protein kinases, and thus may play a role in the response to environmental stress and cytokines. This kinase may also regulate transcription by phosphorylating eIF4E via interaction with the C-terminal region of eIF4G. Alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Jan 2012]
Uniprot Description
May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.
Research Articles on MNK1
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Product Notes
The MNK1 mknk1 (Catalog #AAA852933) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The MNK1 (Phospho-Thr255) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MNK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:1000 ELISA: 1:10000. Researchers should empirically determine the suitability of the MNK1 mknk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MNK1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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