Sequence Information
Lymphotoxin Beta (LTb) Recombinant Protein | LTb recombinant protein
Recombinant Lymphotoxin Beta (LTb)
Gene Names
Ltb; p33; Tnfc; LTbeta; Tnfsf3; AI662801
Reactivity
Mouse
Applications
SDS-Page, Western Blot
Purity
>98%
Synonyms
Lymphotoxin Beta (LTb); Recombinant Lymphotoxin Beta (LTb); LTb recombinant protein
Host
E. coli
Reactivity
Mouse
Purity/Purification
>98%
Form/Format
10mM PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300.
Concentration
original concentration 200µg/mL (varies by lot)
Applicable Applications for LTb recombinant protein
Positive Control, Immunogen, SDS-PAGE, WB
(May be suitable for use in other assays to be determined by the end user.)
(May be suitable for use in other assays to be determined by the end user.)
Source
Prokaryotic expression
Residues
Ile58~Met304
Tags
N-terminal His-Tag
Tissue Specificity
Spleen
Subcellular Location
Membrane; Single-pass type II membrane protein
Traits
Freeze-dried powder
Predicted isoelectric point
6.8
Predicted Molecular Mass
27.6kDa
Accurate Molecular Mass
32kDa as determined by SDS-PAGE reducing conditions
Phenomenon explanation
The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Usage
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Sequence Information
Sequence Information
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
lymphotoxin-beta
NCBI Official Synonym Full Names
lymphotoxin B
NCBI Official Symbol
Ltb
NCBI Official Synonym Symbols
p33; Tnfc; LTbeta; Tnfsf3; AI662801
NCBI Protein Information
lymphotoxin-beta; TNF-C; LT-beta; lymphotoxin beta; tumor necrosis factor C; tumor necrosis factor ligand superfamily member 3
UniProt Protein Name
Lymphotoxin-beta
Protein Family
UniProt Gene Name
Ltb
UniProt Synonym Gene Names
Tnfc; Tnfsf3; LT-beta; TNF-C
UniProt Entry Name
TNFC_MOUSE
Uniprot Description
LTB: Cytokine that binds to LTBR/TNFRSF3. May play a specific role in immune response regulation. Provides the membrane anchor for the attachment of the heterotrimeric complex to the cell surface. Isoform 2 is probably non-functional. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Cellular Component: extracellular space; membrane; plasma membrane; integral to membrane
Molecular Function: cytokine activity; tumor necrosis factor receptor binding
Biological Process: skin development; positive regulation of interleukin-12 biosynthetic process; immune response; gene expression; lymph node development
Research Articles on LTb
Similar Products
Product Notes
The LTb ltb (Catalog #AAA2012386) is a Recombinant Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Lymphotoxin Beta (LTb) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Lymphotoxin Beta (LTb) can be used in a range of immunoassay formats including, but not limited to, Positive Control, Immunogen, SDS-PAGE, WB (May be suitable for use in other assays to be determined by the end user.) . Researchers should empirically determine the suitability of the LTb ltb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lymphotoxin Beta (LTb), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
