Gene Sequencing (extract)
ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) Recombinant Protein | ATP4a recombinant protein
Recombinant ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a)
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Gene Sequencing (extract)
Gene Sequencing (extract)
SDS-Page
SDS-Page
NCBI and Uniprot Product Information
Accurate Molecular Mass: 17kDa as determined by SDS-PAGE reducing conditions.
NCBI Description
The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes a catalytic alpha subunit of the gastric H+, K+-ATPase. [provided by RefSeq, Jul 2008]
Uniprot Description
ATP4A: Catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. Responsible for acid production in the stomach. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
Protein type: Membrane protein, multi-pass; Membrane protein, integral; Energy Metabolism - oxidative phosphorylation; Transporter; Hydrolase; EC 3.6.3.10; Transporter, ion channel
Chromosomal Location of Human Ortholog: 19q13.1
Cellular Component: extracellular space; integral to plasma membrane; plasma membrane
Molecular Function: protein heterodimerization activity; sodium:potassium-exchanging ATPase activity; magnesium ion binding; hydrogen:potassium-exchanging ATPase activity; ATP binding
Biological Process: response to drug; transport; metabolic process; ATP hydrolysis coupled proton transport; pH reduction; regulation of proton transport; transmembrane transport; potassium ion transport
Research Articles on ATP4a
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Product Notes
The ATP4a atp4a (Catalog #AAA2011058) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. The Recombinant ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE; Western BLot (WB). (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the ATP4a atp4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: LTPYLIY ITVSVPLPLG CITILFIELC TDIFPSVSLA YEKAESDIMH LRPRNPKRDR LVNEPLAAYS YFQIGAIQSF AGFTDYFTAM AQEGWFPLLC VG. It is sometimes possible for the material contained within the vial of "ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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