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Application

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10000 results for "stress-protein" - showing 300-350


Immunocytochemistry/Immunofluorescence (ICC/IF) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody, Clone 6F10. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody at 1:400 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) 4-Hydroxy-2-hexenal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)

4-Hydroxy-2-hexenal, Monoclonal Antibody (Cat# AAA808682)

Full Name
4-Hydroxy-2-hexenal Antibody, Clone 6F10
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA, FC/FACS
Purity
Protein G Purified
Pricing
$505/0.1 mg | $2,050/5x0.1 mg
Western Blot (WB) (Western blot analysis of anti-PKCnu C-term Pab in NCI-H460 cell lysate. PKCnu (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

PKC nu, Polyclonal Antibody (Cat# AAA9204329)

Full Name
PKC nu Antibody (C-term)
Gene Names
PRKD3; EPK2; PKD3; PRKCN; PKC-NU; nPKC-NU
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
WB, EIA, IHC-P
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB) (Western blot analysis of Phospho-Sin1 (Ser510) in lysates of MCF7?, using Phospho-Sin1 (Ser510) Antibody.)

Sin1, Polyclonal Antibody (Cat# AAA9610798)

Full Name
Phospho-Sin1 (Ser510) Antibody
Gene Names
MAPKAP1; MIP1; SIN1; JC310; SIN1b; SIN1g
Reactivity
Human, Mouse
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Applications
WB, EIA
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Immunocytochemistry/Immunofluorescence (ICC/IF) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 11E3. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Malondialdehyde Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)

Malondialdehyde, Monoclonal Antibody (Cat# AAA808787)

Full Name
Malondialdehyde Antibody, Clone 11E3: PerCP
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$560/0.1 mg | $2,270/5x0.1 mg
Activity Data (Figure. The binding activity of SIRT3 with IDH2.Sirtuin 3 (SIRT3), the NAD-dependent deacetylaseis is member of the mammalian sirtuin family of proteins. In human, sirtuins have a range of molecular functions and have emerged as important proteins in aging, stress resistance and metabolic regulation. It also can regulate epigenetic gene silencing and suppress recombination of rDNA in yeast. SIRT3 expression in white and brown adipose tissue. Besides, Isocitrate Dehydrogenase 2, mitochondrial (IDH2) has been identified as an interactor of SIRT3, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat SIRT3 and recombinant rat IDH2. Briefly, SIRT3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IDH2-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-SIRT3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of SIRT3 and IDH2 was shown in Figure 1, and this effect was in a dose dependent manner.)

Sirtuin 3 (SIRT3), Active Protein (Cat# AAA2097316)

Full Name
Active Sirtuin 3 (SIRT3)
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$230/0.01 mg | $500/0.05 mg | $770/0.1 mg | $955/0.2 mg | $1,860/0.5 mg | $2,755/1 mg | $6,740/5 mg
Western Blot (WB) (Western blot analysis on NIH-3T3 cell lysate using HSP10 Antibody, The lane on the left is treated with the antigen-specific peptide.)

HSP10, Polyclonal Antibody (Cat# AAA9600121)

Full Name
HSP10 Antibody
Gene Names
HSPE1; EPF; CPN10; GROES; HSP10
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hsp27 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: K562 cell lysate Lane 3.: MCF7 cell lysate The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hsp27 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: A431 cell lysate Lane 2.: HepG2 cell lysate )

Hsp27, Monoclonal Antibody (Cat# AAA200046)

Full Name
Hsp27 antibody
Gene Names
HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
Reactivity
Human
Applications
EIA, WB, FC/FACS, ICC, IF
Purity
By protein-G affinity chromatography
Pricing
$260/0.05 mL | $345/0.1 mL | $1,300/5x0.1 mL
Immunocytochemistry (ICC) (Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-SOD (EC) Polyclonal Antibody . Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-SOD (EC) Polyclonal Antibody at 1:100 for 12 hours at 4 degree C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Golgi lumen. Exosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-SOD (EC) Antibody. (C) Composite.)

SOD (EC), Polyclonal Antibody (Cat# AAA806842)

Full Name
SOD (EC) Antibody: FITC
Gene Names
SOD3; EC-SOD
Reactivity
Human, Mouse, Rat
Applications
WB
Pricing
$500/0.1 mg | $2,020/5x0.1 mg
Immunofluorescence (IF) (MBS9605841 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

G3BP2, Polyclonal Antibody (Cat# AAA9605841)

Full Name
G3BP2 Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (MBS9600234 at 1/100 staining human lung carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

XRCC3, Polyclonal Antibody (Cat# AAA9600234)

Full Name
XRCC3 Antibody
Gene Names
XRCC3; CMM6
Reactivity
Human
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (MBS9605090 staining HepG2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

WWOX, Polyclonal Antibody (Cat# AAA9605090)

Full Name
WWOX Antibody
Gene Names
WWOX; FOR; WOX1; EIEE28; FRA16D; SCAR12; HHCMA56; PRO0128; SDR41C1; D16S432E
Reactivity
Human, Mouse
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (MBS9602025 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

FANCD2, Polyclonal Antibody (Cat# AAA9602025)

Full Name
FANCD2 Antibody
Gene Names
FANCD2; FA4; FAD; FACD; FAD2; FA-D2; FANCD
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL

MHC class I chain-related gene B, Active Protein (Cat# AAA142923)

Full Name
Recombinant Human MHC class I chain-related gene B
Gene Names
MICB; PERB11.2
Purity
Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing
$250/0.01 mg | $4,255/1 mg | $180/0.002 mg | $18,935/5x1 mg
Western Blot (WB) (Western blot analysis of HTRA2 using COLO205 whole lysates.)

HTRA2, Polyclonal Antibody (Cat# AAA9608223)

Full Name
HTRA2 Antibody
Gene Names
HTRA2; OMI; MGCA8; PARK13; PRSS25
Reactivity
Human
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of extracts of 293 and NIH/3T3 cells, using Phospho-MAPK14-Y322 antibody at 1:1000 dilution. 293 cells were treated by UV for 15-30 minutes. NIH/3T3 cells were treated by Anisomycin (25ug/ml) for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 15s.)

MAPK14-Y322, Antibody (Cat# AAA125169)

Full Name
Phospho-MAPK14-Y322 Polyclonal Antibody
Gene Names
MAPK14; RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA
Reactivity
Human, Mouse
Applications
WB, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Immunohistochemistry (IHC) (MBS9600672 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

FoxO3a, Polyclonal Antibody (Cat# AAA9600672)

Full Name
Phospho-FoxO3a (Ser7) Antibody
Gene Names
FOXO3; FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $310/0.2 mL | $1,330/5x0.2 mL
Western Blot (WB) (Western blot analysis of MSK1 phosphorylation expression in HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

MSK1, Polyclonal Antibody (Cat# AAA9600802)

Full Name
Phospho-MSK1 (Ser376) Antibody
Gene Names
RPS6KA5; MSK1; RLPK; MSPK1
Reactivity
Human, Mouse
Applications
WB, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. Inflammatory cells. HSP70/HSC70 stained brown.)

Hsp70/Hsc70, Monoclonal Antibody (Cat# AAA800575)

Full Name
Hsp70/Hsc70 Antibody: FITC
Gene Names
HSPA2; HSP70
Reactivity
Human, Mouse, Rat, Sheep, Dog, Beluga, Bovine, Fish, Guinea pig, Scallop pig, Hamster, Rabbit, Chicken, Xenopus, Drosophila, Yeast
Applications
WB, IP, IHC
Pricing
$520/0.2 mg | $2,135/5x0.2 mg
Western Blot (WB) (Western blot analysis of Human HeLa and HEK293Trap cell lysates showing detection of 47.7 kDa SQSTM1 protein using Rabbit Anti-SQSTM1 Polyclonal Antibody (SPC-637). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-SQSTM1 Polyclonal Antibody (SPC-637) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 47.7 kDa. Other Band(s): ~60 in Jurkat.)

SQSTM1, Polyclonal Antibody (Cat# AAA809653)

Full Name
SQSTM1 Antibody: ATTO 390
Gene Names
SQSTM1; p60; p62; A170; DMRV; OSIL; PDB3; ZIP3; p62B; NADGP; FTDALS3
Reactivity
Human; Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,940/5x0.1 mg
Western Blot (WB) (Western blot analysis of MKK3 expression in MDA-MB-435 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

MKK3, Polyclonal Antibody (Cat# AAA9601896)

Full Name
MKK3 Antibody
Gene Names
MAP2K3; MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
SDS-Page (SDS-PAGE Analysis Purified NRF1 Mouse Monoclonal Antibody (NRF1/2609). Confirmation of Purity and Integrity of Antibody.)

NRF1, Monoclonal Antibody (Cat# AAA4381127)

Full Name
NRF1
Gene Names
NRF1; ALPHA-PAL
Reactivity
Human
Applications
WB, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB) (Western blot analysis of Phospho-HSP27 (Ser82) expression in various lysates)

HSP27, Polyclonal Antibody (Cat# AAA9600854)

Full Name
Phospho-HSP27 (Ser82) Antibody
Gene Names
HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (MBS9601612 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Daxx, Polyclonal Antibody (Cat# AAA9601612)

Full Name
Daxx (Phospho-Ser668) Antibody
Gene Names
DAXX; DAP6; EAP1; BING2
Reactivity
Human, Mouse
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of 50 ug of whole cell lysate (A: mouse brain) using a 15% SDS PAGE gel and NDUFA5 antibody at a dilution of 1:1000)

NDUFA5, Polyclonal Antibody (Cat# AAA5312334)

Full Name
NDUFA5 Antibody
Gene Names
NDUFA5; B13; NUFM; UQOR13; CI-13kB; CI-13KD-B
Applications
IHC, WB
Purity
Antigen-Affinity Chromatography
Pricing
$700/0.1 mL | $3,130/5x0.1 mL
Western Blot (WB) (Western blot analysis of OMI in human colon cell lysates with OMI antibody at (A) 0.5 and (B) 1 μg/mL. )

OMI, Polyclonal Antibody (Cat# AAA150111)

Full Name
OMI Antibody
Gene Names
HTRA2; OMI; PARK13; PRSS25
Reactivity
Human
Applications
EIA, WB, IHC, IF
Purity
OMI Antibody is Ion exchange chromatography purified.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Immunohistochemistry (IHC) (At 1/100 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)

MYPT1, Polyclonal Antibody (Cat# AAA9610466)

Full Name
Phospho-MYPT1 (Ser299) Antibody
Gene Names
PPP1R12A; MBS; M130; MYPT1
Reactivity
Human, Mouse, Rat
Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Applications
WB, IHC, EIA
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Pricing
$245/0.05 mL | $310/0.1 mL | $370/0.2 mL | $1,605/5x0.2 mL
Immunohistochemistry (IHC) (MBS9604416 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

HSF1, Polyclonal Antibody (Cat# AAA9604416)

Full Name
Phospho-HSF1 (Ser326) Antibody
Gene Names
HSF1; HSTF1
Reactivity
Human, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (MBS9601493 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

MAPK10, Polyclonal Antibody (Cat# AAA9601493)

Full Name
MAPK10 Antibody
Gene Names
MAPK10; JNK3; JNK3A; PRKM10; SAPK1b; p493F12; p54bSAPK
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (MBS9602040 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IKK-gamma, Polyclonal Antibody (Cat# AAA9602040)

Full Name
IKK-gamma Antibody
Gene Names
IKBKG; IP; IP1; IP2; FIP3; IKKG; IPD2; NEMO; FIP-3; Fip3p; IMD33; AMCBX1; IKKAP1; ZC2HC9; IKK-gamma
Reactivity
Human, Mouse
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western blot analysis of lysates from Jurkat, mouse NIH/3T3, rat C6, rat PC-12, Hela cell line (from left to right), using RAD51 MBS9231651 Antibody. It was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)

RAD51, Monoclonal Antibody (Cat# AAA9231651)

Full Name
RAD51 Antibody
Gene Names
RAD51; RECA; BRCC5; FANCR; MRMV2; HRAD51; RAD51A; HsRad51; HsT16930
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
This antibody is purified through a protein G column, followed by dialysis against PBS.
Pricing
$435/0.1 mL | $1,655/5x0.1 mL
Immunofluorescence (IF) (Fluorescent image of U251 cells stained with APG8a/b (MAP1LC3A/B) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9214893 APG8a/b (MAP1LC3A/B) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). APG8a/b (MAP1LC3A/B) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)

APG8a/b (MAP1LC3A/B), Polyclonal Antibody (Cat# AAA9214893)

Full Name
APG8a/b (MAP1LC3A/B) Antibody
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, mouse (Predicted Reactivity: Bovine)
Applications
IF, EIA, WB, IHC
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using MAPK8 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.)

MAPK8, Polyclonal Antibody (Cat# AAA125392)

Full Name
MAPK8 Polyclonal Antibody
Gene Names
MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
Reactivity
Human, Mouse
Applications
WB, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant PASK protein (Right) or empty vector (Left) detected with PASK antibody)

PASK, Monoclonal Antibody (Cat# AAA833598)

Full Name
PASK antibody
Gene Names
PASK; STK37; PASKIN
Applications
FC/FACS, IF, IHC, WB
Purity
PASK antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Western Blot (WB) (Western blot analysis of TERT expression in Jurkat cell extracts.The lane on the left is treated with the antigen-specific peptide.)

TERT, Polyclonal Antibody (Cat# AAA9601584)

Full Name
TERT Antibody
Gene Names
TERT; TP2; TRT; CMM9; EST2; TCS1; hTRT; DKCA2; DKCB4; hEST2; PFBMFT1
Reactivity
Human
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (MBS9601693 at 1/100 staining human lung tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at143 degree C.)

MSK1, Polyclonal Antibody (Cat# AAA9601693)

Full Name
MSK1 Antibody
Gene Names
RPS6KA5; MSK1; RLPK; MSPK1
Reactivity
Human, Mouse
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (Western Blot: Sample. Lane1: Mouse Heart Tissue; Lane2: Rat Heart Tissue.)

Mitogen Activated Protein Kinase 14 (MAPK14), Polyclonal Antibody (Cat# AAA2004717)

Full Name
Polyclonal Antibody to Mitogen Activated Protein Kinase 14 (MAPK14)
Gene Names
MAPK14; RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA
Reactivity
Human
Applications
WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM
Purity
Antigen-specific Affinity Chromatography
Pricing
$160/0.1 mL | $195/0.2 mL | $320/0.5 mL | $405/1 mL | $1,160/5x1 mL
Flow Cytometry (FC/FACS) (Flow Cytometric analysis of Hela cells using SERPINB6 antibody (Red), compared to a nonspecific negative control antibody (Blue).)

SERPINB6, Monoclonal Antibody (Cat# AAA833704)

Full Name
SERPINB6 antibody
Gene Names
SERPINB6; CAP; PI6; PTI; PI-6; SPI3; DFNB91; MSTP057
Applications
FC/FACS, IF, WB
Purity
SERPINB6 antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Immunohistochemistry (IHC) (MBS9607489 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

MAPK10, Polyclonal Antibody (Cat# AAA9607489)

Full Name
MAPK10 Antibody
Gene Names
MAPK10; JNK3; JNK3A; PRKM10; SAPK1b; p493F12; p54bSAPK
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (MBS9609048 staining COLO205 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

TP53INP1, Polyclonal Antibody (Cat# AAA9609048)

Full Name
TP53INP1 Antibody
Gene Names
TP53INP1; SIP; Teap; p53DINP1; TP53DINP1; TP53INP1A; TP53INP1B
Reactivity
Human
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) (MBS9600852 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

HSP27, Polyclonal Antibody (Cat# AAA9600852)

Full Name
Phospho-HSP27 (Ser15) Antibody
Gene Names
HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB) (p53DINP1 Antibody)

p53DINP1, Polyclonal Antibody (Cat# AAA151022)

Full Name
p53DINP1 Antibody
Gene Names
TP53INP1; SIP; Teap; p53DINP1; TP53DINP1; TP53INP1A; TP53INP1B
Reactivity
Human, Mouse, Rat
Applications
EIA, IF, IHC-P, WB
Purity
p53DINP1 Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Western Blot (WB) (Western Blot: Sample: Human Brain Tissue.)

Mitogen Activated Protein Kinase 11 (MAPK11), Polyclonal Antibody (Cat# AAA2004868)

Full Name
Polyclonal Antibody to Mitogen Activated Protein Kinase 11 (MAPK11)
Gene Names
MAPK11; P38B; SAPK2; p38-2; PRKM11; SAPK2B; p38Beta; P38BETA2
Reactivity
Human
Applications
WB, ICC, IHC
Purity
Antigen-specific affintiy chromatography followed by Protein A affinity chromatography
Pricing
$160/0.1 mL | $195/0.2 mL | $320/0.5 mL | $405/1 mL | $1,160/5x1 mL
Western Blot (WB) (Western blot analysis of ZRANB1 Antibody (C-term) in K562 cell line lysates (35ug/lane). ZRANB1 (arrow) was detected using the purified Pab.)

ZRANB1, Polyclonal Antibody (Cat# AAA9203740)

Full Name
ZRANB1 Antibody (C-term)
Gene Names
ZRANB1; TRABID
Reactivity
Human, mouse (Predicted Reactivity: Bovine)
Applications
WB, EIA, IHC
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant SIL1 protein (Right) or empty vector (Left) detected with SIL1 antibody)

SIL1, Monoclonal Antibody (Cat# AAA833660)

Full Name
SIL1 antibody
Gene Names
SIL1; BAP; MSS; ULG5
Applications
FC/FACS, IF, IHC, WB
Purity
SIL1 antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Mouse Anti-Nitrotyrosine Monoclonal Antibody, Clone 39B6. Tissue: Retinal Injury Model. Species: Mouse. Primary Antibody: Mouse Anti-Nitrotyrosine Monoclonal Antibody at 1:1000. Secondary Antibody: Alexa Fluor 594 Goat Anti-Mouse (red). Courtesy of: Dr. Rajashekhar Gangaraju, University of Indiana, Department of Ophthalmology, Eugene and Marilyn Glick Eye Institute.)

Nitrotyrosine, Monoclonal Antibody (Cat# AAA801544)

Full Name
Nitrotyrosine Antibody: Biotin
Reactivity
Human, Mouse, Rat, Dog
Applications
WB, EIA, IHC, IP, IF
Pricing
$490/0.1 mg | $1,970/5x0.1 mg
Flow Cytometry (FC/FACS) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant NSMCE2 protein (red) or empty vector (blue) stained using NSMCE2 antibody)

NSMCE2, Monoclonal Antibody (Cat# AAA833430)

Full Name
NSMCE2 antibody
Gene Names
NSMCE2; NSE2; MMS21; ZMIZ7; C8orf36
Applications
FC/FACS, IF, WB
Purity
NSMCE2 antibody was purified by affinity chromatography.
Pricing
$885/0.1 mL | $3,965/5x0.1 mL
Western Blot (WB) (Western Blot Lane1: Human Lung Tissue Lane2: Porcine Muscle Tissue Lane3: Porcine Heart Tissue Primary Ab: 1ug/mL Rabbit Anti-Human HSP27 Ab Second Ab: 1:5000 Dilution of HRP-Linked Rabbit Anti-Mouse IgG Ab )

Heat Shock 27kDa Protein 1, Monoclonal Antibody (Cat# AAA2032033)

Full Name
Heat Shock 27kDa Protein 1 (HSPB1) Monoclonal Antibody
Gene Names
HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102
Reactivity
Human
Applications
WB, ICC, IHC, EIA
Purity
Affinity Chromatography
Pricing
$160/0.1 mL | $205/0.2 mL | $335/0.5 mL | $405/1 mL | $1,190/5x1 mL
Immunohistochemistry (IHC) (MBS9601141 at 1/100 staining human breast carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

HSF1, Polyclonal Antibody (Cat# AAA9601141)

Full Name
Phospho-HSF1 (Ser303) Antibody
Gene Names
HSF1; HSTF1
Reactivity
Human, Mouse
Applications
WB, IHC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunocytochemistry/Immunofluorescence (ICC/IF) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9F11. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Methylglyoxal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)

Methylglyoxal, Monoclonal Antibody (Cat# AAA808823)

Full Name
Methylglyoxal Antibody, Clone 9F11: PerCP
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$560/0.1 mg | $2,270/5x0.1 mg
Immunocytochemistry (ICC) (Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GRP94 Polyclonal Antibody . Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GRP94 Polyclonal Antibody at 1:120 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP94 Antibody. (C) Composite. Heat Shocked at 42 degree C for 1h.)

GRP94, Polyclonal Antibody (Cat# AAA8001978)

Full Name
GRP94 Antibody
Gene Names
Hsp90b1; TA-3; Tra1; gp96; ERp99; GRP94; Targ2; Tra-1; endoplasmin
Reactivity
Human, Mouse, Rat, Bovine
Applications
WB, IHC, ICC, IF, IP
Purity
Peptide Affinity Purified
Pricing
$250/0.025 mg | $895/5x0.025 mg
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