Rabbit GRP94 Polyclonal Antibody | anti-GRP94 antibody

GRP94 Antibody

Cat. Num. AAA8001978

• Gene Names: Hsp90b1; TA-3; Tra1; gp96; ERp99; GRP94; Targ2; Tra-1; endoplasmin
• Reactivity: Human, Mouse, Rat, Bovine
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Peptide Affinity Purified
• Synonyms: GRP94; Polyclonal Antibody; GRP94 Antibody; Rabbit Anti-Mouse GRP94 Polyclonal; HSP90B1 Antibody; GP96 Antibody; TRA1 Antibody; ECGP Antibody; 94 kDa glucose regulated protein antibody; 94 kDa glucose-regulated protein antibody; ECGP antibody; Endoplasmin antibody; Endothelial cell (HBMEC) glycoprotein antibody; ENPL_HUMAN antibody; Glucose regulated protein 94kDa antibody; gp96 antibody; gp96 homolog antibody; GRP 94 antibody; GRP-94 antibody; Heat shock protein 90 kDa beta member 1 antibody; heat shock protein 90kDa beta (Grp94); member 1 antibody; Heat shock protein; 90 kDa; beta; 1 antibody; HSP90B1 antibody; Stress inducible tumor rejection antigen GP96 antibody; TRA1 antibody; tumor rejection antigen (gp96) 1 antibody; Tumor rejection antigen 1 antibody; Tumor rejection antigen gp96 antibody; Tumor rejection antigen-1 (gp96) antibody; anti-GRP94 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Bovine
• Clonality: Polyclonal
• Specificity: Detects ~94kDa.
• Purity/Purification: Peptide Affinity Purified
• Form/Format: PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 802

• Applicable Applications for anti-GRP94 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:1000; ICC/IF: 1:120; IP: 1:80
• Immunogen: Synthetic peptide corresponding to the sequence near the C-terminus of mouse GRP94
• Immunogen Species: Mouse
• Conjugate: Unconjugated
• Cellular Localization: Endoplasmic Reticulum, Endoplasmic Reticulum Lumen, Melanosome
• Certificate of Analysis: 1 ug/ml of SPC-101 was sufficient for detection of Grp94 in 20 ug of Hela lysate by colorimetric immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
• Preparation and Storage: Store at-20 degree C.

Immunocytochemistry (ICC)

(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GRP94 Polyclonal Antibody . Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GRP94 Polyclonal Antibody at 1:120 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP94 Antibody. (C) Composite. Heat Shocked at 42 degree C for 1h.)

Western Blot (WB)

(Western blot analysis of Rat brain cell lysates showing detection of ~ 94-100 kDa GRP94 protein using Rabbit Anti-GRP94 Polyclonal Antibody . Lane 1: MW ladder. Lane 2: Anti-GRP94 (1:250). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-GRP94 Polyclonal Antibody at 1:250 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP antibody at 1:50-1:100 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~ 94-100 kDa. Other Band(s): ~50, ~75 kDa.)

Immunocytochemistry (ICC)/Immunofluorescence (IF)

(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GRP94 Polyclonal Antibody . Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GRP94 Polyclonal Antibody at 1:120 for 12 hours at 4 degree C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP94 Antibody. (C) Composite. Heat Shocked at 42 degree C for 1h.)

Related Product Information for anti-GRP94 antibody

Grp94 (glucose regulated protein 94, gp96) is a constitutively expressed endoplasmic reticulum (ER) lumenal protein that is up-regulated in response to cellular stress such as heat shock, oxidative stress or glucose depletion. Grp94 is thought to play a role in protein translocation to the ER, in their subsequent folding and assembly, and in regulating protein secretion (1). Grp94 also plays a role in antigen presentation by accessing the endogenous pathway and eliciting specific CTL responses to chaperone bound peptides via MHC class I pathway (2). Grp94 is a member of the HSP90 family of stress proteins and shares sequence homology with its cytosolic equivalent, HSP90 (3). Both HSP90 and Grp94 are calcium binding proteins (4). Despite sharing 50% sequence homology over its N domains and complete conservation in its ligand binding domains with HSP90, Grp94 and HSP90 differ in their interactions with regulatory ligands as Grp94 has weak ATP binding and hydrolyisis activity (5). Grp94 exists as a homodimer and the two subunits interact at two distinct intermolecular sites, C terminal dimerization domains and the N-terminal interacts with the middle domain of opposing subunits. (6). Grp94 contains a carboxy terminal KDEL (Lys-Asp-Glu-Leu) sequence which is believed to aid in its retention in the ER (7).

Product Categories/Family for anti-GRP94 antibody

Cancer; Heat Shock; Cell Signaling; Protein Trafficking; ER Proteins; Tags and Cell Markers; Organelle Markers; Cancer; Cancer Metabolism; Response to Hypoxia; Cell Signaling; Metabolism; Metabolism processes; Hypoxia

References

1. Rudolph R.W., and Bedows E. (1997) J Biol Chem 272: 3125-3128. 2. Srivastava P.K., et al. (1994) Immunogenetics. 39(2):93-98. 3. Mazzarella R.A., and Green M. (1987) J Biol Chem 262: 8875-8883. 4. Kang, H.S. and Welch W.J. (1991) J Biol Chem 266(9): 5643-5649. 5. Soldano K.L., et al. (2003) J Biol Chem 278(48): 48330-48338. 6. Chu F., et al. (2006) Protein Sci 15(6): 1260-1269. 7. Peter F., et al., (1992) J Biol Chem 267: 10631-10637. 8. Allen S. et al. (2000) Blood 96(2): 560-568. 9. Sato K et al. (2001) Blood 98(6): 1852-1857. 10. Yun S.-W. et al (2000) Brain Research Bulletin.52(5): 371-378. 11. Choukhi A., et al. (1998) J. Virol. 72: 3851-3858. 12. Hoshino T., et al. (1998) Blood 91(11): 4379-4386. 13. Riera M. et al. (1999) Mol. Cell Biochem. 191: 97-104. 14. Gusarova V., et al. (2001) J. Biol. Chem. 276(27): 24891-24900.

NCBI and Uniprot Product Information

• NCBI GI #: 6755863
• NCBI GeneID: 22027
• NCBI Accession #: NP_035761.1
• NCBI GenBank Nucleotide #: NM_011631.1
• UniProt Accession #: P08113
• NCBI Official Full Name: endoplasmin
• NCBI Official Synonym Full Names: heat shock protein 90, beta (Grp94), member 1
• NCBI Official Symbol: Hsp90b1
• NCBI Official Synonym Symbols: TA-3; Tra1; gp96; ERp99; GRP94; Targ2; Tra-1; endoplasmin
• NCBI Protein Information: endoplasmin
• UniProt Protein Name: Endoplasmin
• UniProt Gene Name: Hsp90b1
• UniProt Synonym Gene Names: Grp94; Tra-1; Tra1; GRP-94; ERp99

Uniprot Description

Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity ().