Western Blot (WB)
(Western blot analysis of 50 ug of whole cell lysate (A: mouse brain) using a 15% SDS PAGE gel and NDUFA5 antibody at a dilution of 1:1000)
Rabbit NDUFA5 Polyclonal Antibody | anti-NDUFA5 antibody
NDUFA5 Antibody
Gene Names
NDUFA5; B13; NUFM; UQOR13; CI-13kB; CI-13KD-B
Applications
Immunohistochemistry, Western Blot
Purity
Antigen-Affinity Chromatography
Synonyms
NDUFA5; Polyclonal Antibody; NDUFA5 Antibody; Affinity Purified Rabbit Polyclonal NDUFA5 Antibody; Polyclonal NDUFA5 antibody; Anti-NDUFA5 antibody; B13 antibody; NDUFA 5 antibody; NDUFA5 antibody; DKFZp781K1356 antibody; FLJ12147 antibody; CI-13kB antibody; UQOR13 antibody; NUFM antibody; NDUFA 5; NADH-ubiquinone oxidoreductase 13 kDa-B subunit antibody; NDUFA-5; NDUFA-5 antibody; type I dehydrogenase antibody; CI-13KD-B antibody; anti-NDUFA5 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen-Affinity Chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
1 mg/ml (varies by lot)
Sequence Length
182
Applicable Applications for anti-NDUFA5 antibody
Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
Application Notes
IHC-P: 1:100-1:500
WB: 1:500-1:3000
WB: 1:500-1:3000
Cross-Reactivity
Human, Mouse
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of 50 ug of whole cell lysate (A: mouse brain) using a 15% SDS PAGE gel and NDUFA5 antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 50 ug of whole cell lysate (A: mouse brain) using a 15% SDS PAGE gel and NDUFA5 antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: Hep G2) using a 15% SDS PAGE gel and NDUFA5 antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: Hep G2) using a 15% SDS PAGE gel and NDUFA5 antibody at a dilution of 1:1000)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded Cal27 xenograft using NDUFA5 antibody at a dilution of 1:500)
Immunohistochemistry (IHC)
(Immunohistochemical staining of paraffin-embedded Cal27 xenograft using NDUFA5 antibody at a dilution of 1:500)
Related Product Information for anti-NDUFA5 antibody
The human NDUFA5 gene codes for the B13 subunit of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. The high degree of conservation of NDUFA5 extending to plants and fungi indicates its functional significance in the enzyme complex. The protein localizes to the inner mitochondrial membrane as part of the 7 component-containing, water soluble "iron-sulfur protein" fraction of complex I, although its specific role is unknown. It is assumed to undergo post-translational removal of the initiator methionine and N-acetylation of the next amino acid. The predicted secondary structure is primarily alpha helix, but the carboxy-terminal half of the protein has high potential to adopt a coiled-coil form. The amino-terminal part contains a putative beta sheet rich in hydrophobic amino acids that may serve as mitochondrial import signal. Related pseudogenes have also been identified on four other chromosomes.
Product Categories/Family for anti-NDUFA5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13 kDa
NCBI Official Full Name
NADH dehydrogenase
NCBI Official Synonym Full Names
NADH:ubiquinone oxidoreductase subunit A5
NCBI Official Symbol
NDUFA5
NCBI Official Synonym Symbols
B13; NUFM; UQOR13; CI-13kB; CI-13KD-B
NCBI Protein Information
NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5
Protein Family
NCBI Description
This nuclear gene encodes a conserved protein that comprises the B13 subunit of complex I of the mitochondrial respiratory chain. The encoded protein localizes to the inner mitochondrial membrane, where it is thought to aid in the transfer of electrons from NADH to ubiquinone. Alternative splicing results in multiple transcript variants. There are numerous pseudogenes of this gene on chromosomes 1, 3, 6, 8, 9, 11, 12, and 16. [provided by RefSeq, Apr 2014]
Research Articles on NDUFA5
Similar Products
Product Notes
The NDUFA5 (Catalog #AAA5312334) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's NDUFA5 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IHC-P: 1:100-1:500 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the NDUFA5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NDUFA5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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