Rabbit p53DINP1 Polyclonal Antibody | anti-TP53INP1 antibody

p53DINP1 Antibody

Cat. Num. AAA151022

• Gene Names: TP53INP1; SIP; Teap; p53DINP1; TP53DINP1; TP53INP1A; TP53INP1B
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: p53DINP1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: p53DINP1; Polyclonal Antibody; p53DINP1 Antibody; SIP; Teap; TP53DINP1; TP53INP1A; TP53INP1B; P53DINP1; Tumor protein p53-inducible nuclear protein 1; Stress-induced protein; tumor protein p53 inducible nuclear protein 1; anti-TP53INP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: At least two isoforms of p53DINP1 are known to exist; this antibody will detect both isoforms.
• Purity/Purification: p53DINP1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid; p53DINP1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 240

• Applicable Applications for anti-TP53INP1 antibody: ELISA (EIA),, Immunofluorescence (IF), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)
• Application Notes: p53DINP1 antibody can be used for detection of p53DINP1 by Western blot at 0.5 - 1 ?g/mL. Antibody can also be used for immunohistochemistry starting at 2 ?g/mL. For immunofluorescence start at 20 ?g/mL.; Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Immunogen: p53DINP1 antibody was raised with a synthetic peptide corresponding to 14 amino acids near the amino terminus of human p53DINP1.; The immunogen is located within the first 50 amino acids of p53DINP1.
• Positive Control: 1) Cat. No. MBS151589 - Human Lung Tissue Lysate
• Preparation and Storage: p53DINP1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(p53DINP1 Antibody)

Immunohistochemistry (IHC)

(Immunohistochemical staining of mouse liver using p53DINP1 antibody at 2 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of p53DINP1 in human liver tissue with p53DINP1 antibody at 5 μg/ml.Green: p53DINP1 antibody (3045)Red: Phylloidin stainingBlue: DAPI staining)

Related Product Information for anti-TP53INP1 antibody

p53DINP1 Antibody: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible gene was identified recently and designated p53DINP1 (for p53-dependent damage-inducible nuclear protein 1) and SIP (for stress induced protein) in human and mouse. A p53DINP1 antisense oligonucleotide inhibits and overexpression of p53DINP1 enhances Ser46 phosphorylation of p53, induction of p53AIP1, and cell death induced by DNA double-strand breaks. p53DINP1 may regulate p53-dependent apoptosis through phosphorylation at Ser46 and induction of p53AIP1. The p53DINP1/SIP gene encodes two proteins of 27 and 18 kDa in human and mouse termed p53DINP1-alpha and p53DINP1-beta or SIP27 and SIP18. p53DINP1/SIP is expressed in many tissues and induced by a variety of stress agents including UV stress, mutagenic stress, heat shock, and oxidative stress.

NCBI and Uniprot Product Information

• NCBI GI #: 61216823
• NCBI GeneID: 94241
• UniProt Accession #: Q96A56; Q969R9; B2RCE5
• Molecular Weight: Predicted: 18, 27 kDa; Observed: 30 kDa
• NCBI Official Full Name: Tumor protein p53-inducible nuclear protein 1
• NCBI Official Synonym Full Names: tumor protein p53 inducible nuclear protein 1
• NCBI Official Symbol: TP53INP1
• NCBI Official Synonym Symbols: SIP; Teap; p53DINP1; TP53DINP1; TP53INP1A; TP53INP1B
• NCBI Protein Information: tumor protein p53-inducible nuclear protein 1; p53-inducible p53DINP1; stress-induced protein; p53-dependent damage-inducible nuclear protein 1
• UniProt Protein Name: Tumor protein p53-inducible nuclear protein 1
• Protein Family: Tumor protein p53-inducible nuclear protein
• UniProt Gene Name: TP53INP1
• UniProt Synonym Gene Names: P53DINP1; SIP; p53DINP1
• UniProt Entry Name: T53I1_HUMAN

Uniprot Description

TP53INP1: Antiproliferative and proapoptotic protein involved in cell stress response which acts as a dual regulator of transcription and autophagy. Acts as a positive regulator of autophagy. In response to cellular stress or activation of autophagy, relocates to autophagosomes where it interacts with autophagosome-associated proteins GABARAP, GABARAPL1/L2, MAP1LC3A/B/C and regulates autophagy. Acts as an antioxidant and plays a major role in p53/TP53-driven oxidative stress response. Possesses both a p53/TP53-independent intracellular reactive oxygen species (ROS) regulatory function and a p53/TP53-dependent transcription regulatory function. Positively regulates p53/TP53 and p73/TP73 and stimulates their capacity to induce apoptosis and regulate cell cycle. In response to double-strand DNA breaks, promotes p53/TP53 phosphorylation on 'Ser-46' and subsequent apoptosis. Acts as a tumor suppressor by inducing cell death by an autophagy and caspase-dependent mechanism. Can reduce cell migration by regulating the expression of SPARC. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Apoptosis; Tumor suppressor

Chromosomal Location of Human Ortholog: 8q22

Cellular Component: PML body; autophagic vacuole; cytoplasmic vesicle; nucleus; cytosol

Molecular Function: antioxidant activity; protein binding

Biological Process: negative regulation of cell proliferation; transcription, DNA-dependent; apoptosis; response to heat; positive regulation of transcription, DNA-dependent; autophagic cell death; response to stress; cell cycle arrest; negative regulation of cell migration; positive regulation of autophagy; autophagic vacuole formation