Rabbit anti-Human OMI Polyclonal Antibody | anti-HTRA2 antibody

OMI Antibody

Cat. Num. AAA150111

• Gene Names: HTRA2; OMI; PARK13; PRSS25
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: OMI Antibody is Ion exchange chromatography purified.
• Synonyms: OMI; Polyclonal Antibody; OMI Antibody; PARK13; PRSS25; Serine protease HTRA2; mitochondrial; High temperature requirement protein A2; HtrA2; HtrA serine peptidase 2; anti-HTRA2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: OMI Antibody is Ion exchange chromatography purified.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 458

• Applicable Applications for anti-HTRA2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: OMI antibody can be used for detection of OMI by Western blot at 0.5 to 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: OMI antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human OMI.
• Buffer: OMI Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: OMI antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of OMI in human colon cell lysates with OMI antibody at (A) 0.5 and (B) 1 μg/mL. )

Immunohistochemistry (IHC)

(Immunohistochemistry of OMI in human colon tissue with OMI antibody at 10 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of OMI in human colon tissue with OMI antibody at 20 μg/mL.)

Related Product Information for anti-HTRA2 antibody

OMI Antibody: Inhibitor of apoptosis proteins (IAPs) were initially identified in baculoviruses as proteins that inhibit apoptosis of the host cells to allow time for viral replication. Cellular homologues containing at least one baculoviral IAP repeat (BIR) motif essential for their anti-apoptosis activity have been identified in yeasts and higher organisms and often act by binding and inhibiting processed caspases. The activity of these proteins can be modulated by the expression of proteins such as Smac/DIABLO and XAF-1 which displace or prevent the binding of caspases by IAPs. Recently, a mitochondrial serine protease termed Omi/HtrA2 has been found to bind IAPs. Similar to Smac, Omi possesses a conserved IAP-binding motif, but acts to cleave IAPs to irreversibly inactivate IAPs and promote apoptosis.

NCBI and Uniprot Product Information

• NCBI GI #: 5870865
• NCBI GeneID: 27429
• UniProt Accession #: O43464; Q9HBZ4; Q9P0Y3; Q9P0Y4
• Molecular Weight: 39,914 Da
• NCBI Official Full Name: serine protease
• NCBI Official Synonym Full Names: HtrA serine peptidase 2
• NCBI Official Symbol: HTRA2
• NCBI Official Synonym Symbols: OMI; PARK13; PRSS25
• NCBI Protein Information: serine protease HTRA2, mitochondrial; serine protease 25; protease, serine, 25; serine proteinase OMI; HtrA-like serine protease; Omi stress-regulated endoprotease; high temperature requirement protein A2
• UniProt Protein Name: Serine protease HTRA2, mitochondrial
• Protein Family: Serine protease
• UniProt Gene Name: HTRA2
• UniProt Synonym Gene Names: OMI; PRSS25; HtrA2
• UniProt Entry Name: HTRA2_HUMAN

NCBI Description

This gene encodes a serine protease. The protein has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional transcript variants have been described, but their full-length sequences have not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

HTRA2: a serine proteinase that normally resides in mitochondrial membranes. It exists in two populations in mitochondria: as an unprocessed form probably attached to the inner mitochondrial membrane through a N-terminal transmembrane domain, and as a processed form containing a reaper motif at its N-terminus. Following apoptotic stimuli it is autoproteolytically activated and released into the cytosol, where it promotes programmed cell death in caspase-dependent and -independent manners. The amino-terminal reaper motif binds to the IAP (inhibitor of apoptosis) proteins cIAP1, cIAP2, and and XIAP, disrupting IAP-caspase complexes in a manner similar to Smac/DIABLO. Phosphorylation by the protein kinase Akt attenuates its serine protease and pro-apoptotic activities. The PDZ domain mediates interaction with Mxi2, an alternatively spliced form of the p38 stress-activated kinase. In contrast to its pro-apoptotic effects, targeted deletion in mice indicates that it is involved in protection against cell stress in striatial neurons. Defects in HTRA2 are the cause of Parkinson disease 13 (PARK13). Four alternatively spliced human isoforms have been described.

Protein type: Membrane protein, integral; Protease; EC 3.4.21.108; Mitochondrial

Chromosomal Location of Human Ortholog: 2p12

Cellular Component: endoplasmic reticulum membrane; internal side of plasma membrane; cytoskeleton; membrane; mitochondrion; endoplasmic reticulum; mitochondrial membrane; mitochondrial intermembrane space; cytosol; chromatin; nucleus

Molecular Function: peptidase activity; protein binding; serine-type peptidase activity; serine-type endopeptidase activity; unfolded protein binding

Biological Process: mitochondrion organization and biogenesis; positive regulation of apoptosis; positive regulation of caspase activity; regulation of multicellular organism growth; response to herbicide; proteolysis; adult walking behavior; negative regulation of cell cycle; protein autoprocessing; DNA damage response, signal transduction resulting in induction of apoptosis; pentacyclic triterpenoid metabolic process; cellular protein catabolic process; forebrain development; neuron development; ceramide metabolic process

Disease: Parkinson Disease 13, Autosomal Dominant, Susceptibility To