Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
10000 results for "actin" - showing 300-350
Immunofluorescence (IF)
(Confocal immunofluorescence analysis of Hela cells using MLH1 antibody (green), showing nuclear localization. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
MLH1, Monoclonal Antibody (Cat# AAA5310879)
Full Name
MLH1 antibody
Gene Names
MLH1; FCC2; COCA2; HNPCC; hMLH1; HNPCC2
Applications
EIA, IF, IHC, WB
Pricing
$605/0.1 mL | $2,715/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H. cerebellum section using GLD2 Antibody (N-term). MBS9212203 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
GLD2, Polyclonal Antibody (Cat# AAA9212203)
Full Name
GLD2 Antibody (N-term)
Gene Names
PAPD4; GLD2; TUT2
Applications
EIA, IHC-P, IF, WB
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB)
(Park2 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the Park2 antibody detected the Park2 protein (arrow).)
Parkin, Polyclonal Antibody (Cat# AAA9203814)
Full Name
Parkin Antibody (C-term)
Gene Names
PARK2; PDJ; PRKN; AR-JP; LPRS2
Reactivity
Human, mouse
Applications
WB, EIA, IF, FC/FACS, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Typical Standard Curve/Testing Data
Actin Alpha 2, Smooth Muscle (ACTa2), ELISA Kit (Cat# AAA450851)
Full Name
Human Actin Alpha 2, Smooth Muscle (ACTa2) ELISA Kit
Gene Names
ACTA2; AAT6; ACTSA; MYMY5
Reactivity
Human
Pricing
$425/48-Strip-Wells | $565/96-Strip-Wells | $2,540/5x96-Strip-Wells | $4,955/10x96-Strip-Wells
Western Blot (WB)
(GSTP1 Antibody (Center) western blot analysis in K562,PC-3,Y79 cell line and mouse lung tissue lysates (35ug/lane).This demonstrates the GSTP1 antibody detected the GSTP1 protein (arrow).)
GSTP1, Polyclonal Antibody (Cat# AAA9215166)
Full Name
GSTP1 Antibody (Center)
Gene Names
GSTP1; PI; DFN7; GST3; GSTP; FAEES3; HEL-S-22
Reactivity
Human, mouse
Applications
IF, EIA, WB, IHC, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(Western blot analysis of ACTL6B using HeLa whole cell lysates)
ACTL6B, Polyclonal Antibody (Cat# AAA9609389)
Full Name
ACTL6B Antibody
Gene Names
ACTL6B; ACTL6; BAF53B; arpNalpha
Reactivity
Human, Mouse
Applications
WB, EIA, ICC, IF
Purity
Peptide affinity purification
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA210664)
Full Name
MOUSE ANTI V5-TAG:FITC
Applications
IF
Pricing
$445/0.1 mg | $1,845/5x0.1 mg
Typical Testing Data/Standard Curve (for reference only)
HLTF, ELISA Kit (Cat# AAA2511303)
Full Name
Human HLTF (Helicase Like Transcription Factor) ELISA Kit
Gene Names
HLTF; ZBU1; HLTF1; RNF80; HIP116; SNF2L3; HIP116A; SMARCA3
Reactivity
Human
Pricing
$440/48-Strip-Wells | $525/96-Strip-Wells | $2,215/5x96-Strip-Wells | $3,965/10x96-Strip-Wells | $250/24-Strip-Wells
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded Rat Lung stained with Smooth Muscle Actin Monoclonal Antibody (1A4 + ACTA2/791).)
Actin, Monoclonal Antibody (Cat# AAA439602)
Full Name
Actin, Smooth Muscle (Leiomyosarcoma Marker) Mouse Monoclonal Antibody
Gene Names
ACTA2; AAT6; ACTSA; MYMY5
Reactivity
Human, Rat.
Predicted to show a broad reactivity
Predicted to show a broad reactivity
Applications
FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with FMR1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
FMR1, Polyclonal Antibody (Cat# AAA9207834)
Full Name
FMR1 Antibody (N-term)
Gene Names
FMR1; POF; FMRP; POF1; FRAXA
Reactivity
Human, mouse
Applications
IHC-P, WB, FC,E
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
PIP, cDNA Clone (Cat# AAA1271743)
Full Name
PIP cDNA Clone
Gene Names
PIP; GPIP4; GCDFP15; GCDFP-15
Pricing
$845/5x0.01 mg Plasmid + 5x0.2 mL Glycerol-Stock | $190/0.01 mg Plasmid + 0.2 mL Glycerol-Stock
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Uterus stained with Calponin-1 Monoclonal Antibody (SPM169).)
Calponin-1, Monoclonal Antibody (Cat# AAA438913)
Full Name
Calponin-1 (Smooth Muscle Marker) Mouse Monoclonal Antibody
Gene Names
CNN1; SMCC; Sm-Calp; HEL-S-14
Reactivity
Human, Rat
Applications
FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Western blot detection of beta actin in Hela, 3T3, COS7, CHO-k1 and Goat muscle cell lysates using beta actin mouse mAb (1:1500 diluted).Predicted band size:45KDa.Observed band size:45KDa.)
beta-Actin, Monoclonal Antibody (Cat# AAA475054)
Full Name
Anti-beta-Actin Mouse mAb
Gene Names
ACTB; BRWS1; PS1TP5BP1
Reactivity
Human, Rat, Mouse, Goat, Monkey, Hamster
Applications
WB
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Immunohistochemistry (IHC)
(Formalin-fixed. Paraffin-embedded human Melanoma stained with S100B Mouse Monoclonal Antibody (S100B/1012).)
S100B, Monoclonal Antibody (Cat# AAA4380220)
Full Name
S100B (Astrocyte and Melanoma Marker)
Gene Names
S100B; NEF; S100; S100-B; S100beta
Reactivity
Human, Mouse, Rat, Cow. Others not known.
Applications
FC/FACS, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Western blot analysis of C19orf21 Antibody (N-term) (MBS9206968) in HepG2 cell line lysates (35ug/lane). C19orf21 (arrow) was detected using the purified Pab.)
C19orf21, Polyclonal Antibody (Cat# AAA9206968)
Full Name
C19orf21 Antibody (N-term)
Gene Names
MISP; C19orf21
Reactivity
Human
Applications
WB, EIA, IHC, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Typical Testing Data/Standard Curve (for reference only)
TAGLN, ELISA Kit (Cat# AAA2508359)
Full Name
Human TAGLN (Transgelin) ELISA Kit
Gene Names
TAGLN; SM22; SMCC; TAGLN1; WS3-10
Reactivity
Human
Pricing
$440/48-Strip-Wells | $525/96-Strip-Wells | $2,215/5x96-Strip-Wells | $3,965/10x96-Strip-Wells | $250/24-Strip-Wells
Western Blot (WB)
(Western Blot analysis of extracts of various cells line, using B-actin antibody MBS125142 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:1000 dilution.Lysates/proteins: 25 ug/per lane. Blocking buffer: 3% non fat dry milk in TBST.Detection: ECL Basic Kit Exposure time: 90s.)
beta-actin, Polyclonal Antibody (Cat# AAA125142)
Full Name
beta-actin Polyclonal Antibody
Gene Names
ACTB; BRWS1; PS1TP5BP1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$175/0.05 mL | $215/0.1 mL | $285/0.2 mL | $1,130/5x0.2 mL
Western Blot (WB)
((0.3ug/ml) staining of MCF7 nuclear (A) and U251 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)
SOX2, Antibody (Cat# AAA423840)
Full Name
Goat Anti-SOX2 Antibody
Gene Names
SOX2; ANOP3; MCOPS3
Reactivity
Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Applications
EIA, WB, IF, FC
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
$335/0.1 mg
Testing Data
(Sequence)
Fibronectin (FN), Active Protein (Cat# AAA2097257)
Full Name
Active Fibronectin (FN)
Gene Names
FN1; FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2; SMDCF
Applications
Cell culture, Activity Assays
Purity
>98%
Pricing
$175/0.01 mg | $335/0.05 mg | $510/0.1 mg | $625/0.2 mg | $1,205/0.5 mg | $1,790/1 mg | $4,385/5 mg
Immunohistochemistry (IHC)
(Anti-AKT1/2/3 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)
AKT1 + AKT2 + AKT3, Polyclonal Antibody (Cat# AAA242291)
Full Name
Rabbit Polyclonal to Human AKT1 + AKT2 + AKT3
Reactivity
Chicken, Human, Mouse, Rat
Applications
IHC - Paraffin, IF, WB, IP, EIA
Purity
Delipidated and defibrinated
Pricing
$585/0.05 mL | $2,585/5x0.05 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of MBS9203877 on paraffin-embedded human kidney tissue was performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:250) for 15min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary antibody.)
NEU2, Polyclonal Antibody (Cat# AAA9203877)
Full Name
NEU2 Antibody (N-term)
Gene Names
NEU2; SIAL2
Reactivity
Human
Applications
WB, EIA, IHC-P-Leica, IF, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunohistochemistry (IHC)
(Anti-MYOD / MYOD1 antibody IHC staining of human small intestine, muscularis propria. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
MYOD / MYOD1, Polyclonal Antibody (Cat# AAA248172)
Full Name
Rabbit Polyclonal to Human MYOD / MYOD1
Gene Names
MYOD1; PUM; MYF3; MYOD; bHLHc1
Reactivity
Human
Applications
IHC - Paraffin, IF, WB
Purity
Immunoaffinity Purified
Pricing
$610/0.2 mL | $2,685/5x0.2 mL
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Skeletal Muscle stained with Dystrophin Monospecific Mouse Monoclonal Antibody (DMD/3245).)
Dystrophin (DMD), Monoclonal Antibody (Cat# AAA4380840)
Full Name
Dystrophin (DMD) (Marker of Duchenne and Becker Muscular Dystrophy)
Gene Names
DMD; BMD; CMD3B; MRX85; DXS142; DXS164; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; DXS272
Reactivity
Human
Applications
EIA, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Figure 1 Western Blot Validation in Human Cell LinesLoading: 15 μg of lysates per lane.Antibodies: TIM-1 3811 (1 μg /mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
TIM-1, Polyclonal Antibody (Cat# AAA150460)
Full Name
TIM-1 Antibody
Gene Names
HAVCR1; TIM; KIM1; TIM1; HAVCR; KIM-1; TIM-1; TIMD1; TIMD-1; HAVCR-1
Reactivity
Human, Rat
Applications
EIA, WB
Purity
TIM-1 Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Testing Data
(25 ug of HeLa cell lysate spiked with 3.5 ug of mouse IgG was run in lanes 2, 3, 5 and 6 under reducing conditions on SDS PAGE using the V3 system and transferred to PVDF membrane. Precision Plus MW marker was run in lanes 1 and 4. Mouse anti actin gamma (VMA00049) was used to detect actin gamma (approximate MW of 46 kDa) in lanes 2 and 5 only. Lanes 3 and 6 were used as controls to detect the background signaling of the secondary detection agent (no primary antibody control).. TidyBlot Western Blot Detection Reagent:HRP was used as the secondary reagent in lanes 2 and 3 and an HRP conjugated anti-mouse IgG heavy and light chain antibody was used as the secondary in lanes 5 and 6. The Blot was visualized with the Chemidoc. The TidyBlot Western Blot Detection Reagent:HRP only bound to the native actin gamma antibody whereas the standard anti-Mouse IgG heavy and light recognized both the native and denatured antibodies)
TidyBlot WESTERN BLOT DETECTION REAGENT, Accessory Reagent (Cat# AAA224768)
Full Name
TidyBlot WESTERN BLOT DETECTION REAGENT: HRP
Applications
WB
Pricing
$170/0.05 mL | $220/0.5 mL | $325/1 mL | $845/5x0.5 mL | $1,305/5x1 mL | $625/5x0.05 mL
Western Blot (WB)
(Figure 1. Western blot analysis using CHK2 mouse mAb against cell lysate from various cell types.)
CHK2, Monoclonal Antibody (Cat# AAA850536)
Full Name
CHK2 Antibody
Gene Names
CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
Reactivity
Human
Applications
WB, IHC, IF, EIA
Pricing
$310/0.1 mg | $1,330/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with Muscle Specific Actin Monoclonal Antibody (SPM160))
Actin, Monoclonal Antibody (Cat# AAA438064)
Full Name
Actin, Muscle Specific (Muscle Cell Marker) Mouse Monoclonal Antibody
Gene Names
ACTA2; AAT6; ACTSA; MYMY5
Reactivity
Human, Rabbit, Rat
Applications
FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Transgelin Monoclonal Antibody (TAGLN/247))
Transgelin, Monoclonal Antibody (Cat# AAA439392)
Full Name
Transgelin (SM22-alpha) Mouse Monoclonal Antibody
Gene Names
TAGLN; SM22; SMCC; TAGLN1; WS3-10
Reactivity
Human, Cow, Pig, Rabbit, Mouse
Applications
FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA at 1mg/mL)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Melanoma stained with S100B Mouse Monoclonal Antibody (4C4.9) (AEC Chromogen))
S100B, Monoclonal Antibody (Cat# AAA4380217)
Full Name
S100B (Astrocyte and Melanoma Marker)
Gene Names
S100B; NEF; S100; S100-B; S100beta
Reactivity
Human, Mouse, Rat, Cow. Others not known.
Applications
FC/FACS, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Ep-CAM Monoclonal Antibody (EGP40/826).)
Ep-CAM /CD326, Monoclonal Antibody (Cat# AAA438771)
Full Name
Ep-CAM /CD326 (Epithelial Marker) Mouse Monoclonal Antibody
Gene Names
EPCAM; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
Reactivity
Human.
Does not react with Mouse and Rat
Does not react with Mouse and Rat
Applications
FC/FACS, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded Human Placenta stained with Spectrin alpha 1 (SPTA1) Mouse Monoclonal Antibody (SPTA1/1832).)
Spectrin Alpha 1, Monoclonal Antibody (Cat# AAA4380492)
Full Name
Spectrin Alpha 1 (Erythrocyte Marker)
Gene Names
SPTA1; EL2; HPP; HS3; SPH3; SPTA
Reactivity
Human. Others not known.
Applications
EIA, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 4F1. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm: medium; Nucleus: strong. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha Synuclein Antibody. (D) Composite.)
Alpha Synuclein, Monoclonal Antibody (Cat# AAA808971)
Full Name
Alpha Synuclein Antibody, Clone 4F1: ATTO 390
Gene Names
Snca; NACP; alphaSYN; alpha-Syn
Reactivity
Human; Mouse; Rat
Applications
WB, DB, IHC, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB)
(Western blot analysis of lysates from NIH/3T3 cell line, untreated or treated with EGF, 100ng/ml, using Phospho-SHP2-Y546 Antibody(EV)(upper) or Beta-actin (lower).)
SHP2, Polyclonal Antibody (Cat# AAA9231732)
Full Name
Phospho-SHP2 (Y546) Antibody
Gene Names
PTPN11; CFC; NS1; JMML; SHP2; BPTP3; PTP2C; METCDS; PTP-1D; SH-PTP2; SH-PTP3
Reactivity
Human, Mouse
Predicted: Chicken, Rat
Predicted: Chicken, Rat
Applications
WB, DB, EIA
Pricing
$435/0.1 mL | $1,655/5x0.1 mL
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Calponin-1 Monoclonal Antibody (CNN1/832).)
Calponin-1, Monoclonal Antibody (Cat# AAA438657)
Full Name
Calponin-1 (Smooth Muscle Marker) Mouse Monoclonal Antibody
Gene Names
CNN1; SMCC; Sm-Calp; HEL-S-14
Reactivity
Human, Rat
Applications
FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 4F1. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm: medium; Nucleus: strong. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha Synuclein Antibody. (D) Composite.)
Alpha Synuclein, Monoclonal Antibody (Cat# AAA808972)
Full Name
Alpha Synuclein Antibody, Clone 4F1: ATTO 488
Gene Names
Snca; NACP; alphaSYN; alpha-Syn
Reactivity
Human; Mouse; Rat
Applications
WB, DB, IHC, ICC, IF, EIA
Purity
Protein G Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 μg of lysates per lane.Antibodies: ATG5 5031 (2 μg/mL), ATG5 64-229 (2 μg/mL), ????-actin (1 μg/mL), and GAPDH (0.02 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-chicken or goat anti-mouse IgG HRP conjugate at 1:10000 and 1:5000 dilution, respectively.)
ATG5, Polyclonal Antibody (Cat# AAA151098)
Full Name
ATG5 Antibody
Gene Names
ATG5; ASP; APG5; APG5L; hAPG5; APG5-LIKE
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC
Purity
ATG5 Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Testing Data
(Western blotting analysis of human SOS1 epression on K562 (lane 1) and Raji (lane 2) cell lysates usingMouse anti Human SOS1 antibody, detection is by chemiluminescence)
SOS1, Monoclonal Antibody (Cat# AAA217406)
Full Name
MOUSE ANTI SOS1
Gene Names
SOS1; GF1; HGF; NS4; GGF1; GINGF
Reactivity
Mouse
Applications
EIA, IF, WB
Pricing
$345/0.1 mg | $1,405/5x0.1 mg
Immunofluorescence (IF)
(Fluorescent image of Hela cell stained with IRS2 Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with IRS2 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree).IRS2 immunoreactivity is localized to Cytoplasm significantly.)
IRS2, Polyclonal Antibody (Cat# AAA9213098)
Full Name
IRS2 Antibody (C-term)
Gene Names
IRS2; IRS-2
Reactivity
Human
Applications
IF, EIA, WB, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Uterus stained with Calponin-1 Recombinant Mouse Monoclonal Antibody (rCNN1/832).)
Calponin-1, Monoclonal Antibody (Cat# AAA439885)
Full Name
Calponin-1 (Smooth Muscle Marker)
Gene Names
CNN1; SMCC; Sm-Calp; HEL-S-14
Reactivity
Human, Rat
Applications
FC, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
SDS-PAGE
Actin, cytoplasmic 1 (ACTB), Recombinant Protein (Cat# AAA962728)
Full Name
Recombinant Human Actin, cytoplasmic 1 (ACTB)
Gene Names
ACTB; BRWS1; PS1TP5BP1
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,605/1 mg (E-Coli) | $1,075/0.1 mg (Baculovirus) | $450/0.1 mg (E-Coli) | $440/0.02 mg (Baculovirus) | $1,475/0.5 mg (Baculovirus) | $265/0.02 mg (E-Coli) | $7,045/5x1 mg (E-Coli) | $6,460/5x0.5 mg (Baculovirus)
Western Blot (WB)
(The anti-phospho CDC25A S124 Pab is used in Western blot to detect CDC25A in mouse liver tissue lysate)
Phospho-CDC25A (S124), Polyclonal Antibody (Cat# AAA9201824)
Full Name
Phospho-CDC25A (S124) Antibody
Gene Names
CDC25A; CDC25A2
Reactivity
Human (Predicted Reactivity: Rat)
Applications
WB, EIA, IHC
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB)
(The CDK7-T170 Pab is used in Western blot to detect CDK7-T170 in Ramos tissue lysate.)
Phospho-CDK7 (T170), Polyclonal Antibody (Cat# AAA9201280)
Full Name
Phospho-CDK7 (T170) Antibody
Gene Names
CDK7; CAK1; HCAK; MO15; STK1; CDKN7; p39MO15
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
WB, DB, EIA, IHC-p
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunofluorescence (IF)
(Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Flightless Iantibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.)
Flightless I, Polyclonal Antibody (Cat# AAA837309)
Full Name
Flightless I antibody
Gene Names
fliI; 15; BcDNA:LD21753; CG1484; CT3691; DCA3-19; DmelCG1484; EN3; fli; fli I; fli-I; Fli-I; FLI-I; fltI; fltO; l(1)18-80; l(1)19Fd; l(1)ESHS53; l(1)W-2; lDCB2-19; sdby; w-2; W2
Applications
IF, ICC, WB
Purity
Flightless I antibody was purified by antigen-affinity chromatography
Pricing
$700/0.1 mL | $3,130/5x0.1 mL
Western Blot (WB)
(Western Blot Analysis of HeLa and 293 Cell Lysates using Spectrin beta III Monoclonal Antibody (SPTBN2/1582).)
Spectrin beta III (SPTBN2), Monoclonal Antibody (Cat# AAA4380235)
Full Name
Spectrin beta III (SPTBN2)
Gene Names
SPTBN2; SCA5; SCAR14; GTRAP41
Reactivity
Human. Others not known.
Applications
EIA, FC/FACS, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(C9JLR9 Antibody (C-term) (MBS9215006) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the C9JLR9 antibody detected the C9JLR9 protein (arrow).)
C9JLR9, Polyclonal Antibody (Cat# AAA9215006)
Full Name
C9JLR9 Antibody (C-term)
Reactivity
Human
Predicted Reactivity: Mouse
Predicted Reactivity: Mouse
Applications
WB, IHC-P, IF
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded Rat Heart stained with Muscle Specific Actin Monoclonal Antibody (HHF35 + MSA/953))
Actin, Monoclonal Antibody (Cat# AAA438839)
Full Name
Actin, Muscle Specific (Muscle Cell Marker) Mouse Monoclonal Antibody
Gene Names
ACTA2; AAT6; ACTSA; MYMY5
Reactivity
Human, Rabbit, Rat
Applications
FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with Fascin-1 Monoclonal Antibody (FSCN1/416))
Fascin-1, Monoclonal Antibody (Cat# AAA439680)
Full Name
Fascin-1 (Reed-Sternberg Cell Marker) Mouse Monoclonal Antibody
Gene Names
FSCN1; HSN; SNL; p55; FAN1
Reactivity
Human
Applications
FC/FACS, IF, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunofluorescence (IF)
(Fluorescent confocal image of HeLa cells stained with phospho-SMAD3-S208 antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9213657 phospho-SMAD3-S208 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 uM, 25 min). Pictures were taken on a Biorevo microscope (BZ-900, Keyence).Note the highly specific localization of the phospho-SMAD3 mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000166949).)
Phospho-SMAD3 (S208), Polyclonal Antibody (Cat# AAA9213657)
Full Name
Phospho-SMAD3 (S208) Antibody
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human (Predicted Reactivity: Chicken, Mouse, Pig, Rat)
Applications
IF, DB, EIA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Immunohistochemistry (IHC)
(MBS9603401 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Synaptopodin, Polyclonal Antibody (Cat# AAA9603401)
Full Name
Synaptopodin Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Melanoma stained with S100B Mouse Monoclonal Antibody (4C4.9 + S100B/1012))
S100B, Monoclonal Antibody (Cat# AAA4380221)
Full Name
S100B (Astrocyte and Melanoma Marker)
Gene Names
S100B; NEF; S100; S100-B; S100beta
Reactivity
Human, Mouse, Rat, Cow. Others not known.
Applications
FC/FACS, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
