Rabbit AKT1 + AKT2 + AKT3 Polyclonal Antibody | anti-AKT1 + AKT2 + AKT3 antibody

Rabbit Polyclonal to Human AKT1 + AKT2 + AKT3

Cat. Num. AAA242291

• Reactivity: Chicken, Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, Immunoprecipitation, ELISA
• Purity: Delipidated and defibrinated
• Synonyms: AKT1 + AKT2 + AKT3; Polyclonal Antibody; Rabbit Polyclonal to Human AKT1 + AKT2 + AKT3; Anti-AKT1 + AKT2 + AKT3 Antibody (C-Terminus) IHC-plus; Human AKT1 + AKT2 + AKT3; anti-AKT1 + AKT2 + AKT3 antibody

• Host: Rabbit
• Reactivity: Chicken, Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Synthetic peptide C-R-P-H-F-P-Q-F-S-Y-S-A-S-G-T-A corresponding to the C-terminus (460-480) of human, rat and mouse and chicken AKT proteins conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.
• Purity/Purification: Delipidated and defibrinated
• Form/Format: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.1% sodium azide.
• Concentration: 75 mg/ml (varies by lot)

• Applicable Applications for anti-AKT1 + AKT2 + AKT3 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA)
• Application Notes: ELISA (1:2000 - 1:10000), IF (1:100 - 1:1000), IHC-P (1:100), IP, WB (1:500 - 1:2000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human AKT (AKT1/AKT2/AKT3).
• Immunogen Type: Synthetic peptide
• Immunogen: Synthetic peptide from human AKT (AKT1/AKT2/AKT3).
• Antigen Modification: C-Terminus
• Preparation and Storage: Store vial at -20 degree C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing.

Immunohistochemistry (IHC)

(Anti-AKT1/2/3 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)

Immunofluorescence (IF)

(Immunofluorescence microscopy. Rabbit anti-AKT was used at a 1:80 dilution to stain cultured neonatal rat cardiomyocytes that express a nuclear-targeted AKT construct. Anti-AKT staining appears green. Actin filaments are labeled red using a Texas-red conjugated phalloidin.)

Immunocytochemistry (ICC)

(Immunofluorescence Microscopy of Rabbit Anti-AKT Antibody. Tissue: neonatal rat cardiomyocytes. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: AKT antibody at 1:80 dilution for 1 h at RT. Secondary antibody: Texas-red conjugated rabbit secondary antibody at 1:10000 for 45 min at RT. Localization: AKT is nuclear. Staining: Anti-AKT staining appears green. Actin filaments are labeled red using a Texas-red conjugated phalloidin.)

Western Blot (WB)

(Western Blot. Rabbit anti-AKT was used at a 1:500 dilution to detect AKT by Western blot.)

Western Blot (WB)

(Western blot of Rabbit Anti-AKT antibody. Lane 1: NIH/3T3 whole cell lysate. Load: 20 ug per lane. Primary antibody: AKT antibody at 1:500 for overnight at 4C. Secondary antibody: HRP conjugated Gt-a-Rabbit IgG at 1:10000 preceded color development using Pierce Chemical's SuperSignal substrate. Block: MOPS buffer overnight at 4C. Predicted/Observed size: 128 kDa, 128 kDa for AKT. Other band(s): none.)