Goat SOX2 Antibody | anti-SOX2 antibody

Goat Anti-SOX2 Antibody

Cat. Num. AAA423840

• Gene Names: SOX2; ANOP3; MCOPS3
• Reactivity: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
• Applications: ELISA, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: SOX2; Goat Anti-SOX2 Antibody; SRY (sex determining region Y)-box 2; ANOP3; MGC2413 ; SRY-related HMG-box gene 2; sex-determining region Y-box 2; transcription factor SOX2; anti-SOX2 antibody

• Host: Goat
• Reactivity: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Sequence Length: 317

• Applicable Applications for anti-SOX2 antibody: Peptide ELISA (EIA), WB, Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Application Notes: Peptide ELISA: antibody detection limit dilution 1:32000; WB: Approx 37kDa band observed in nuclear lysates of cell line MCF7 and in lysates of Human glioblastoma cell line U251 (calculated MW of 34.3kDa according to)NP_003097.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation 1 hour at room temperature.; IF: Strong expression of the protein seen in the nuclei of U251 cells. Recommended concentration: 10ug/ml.; FC/FACS: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.
• Immunogen: Peptide with sequence YNMMETELKPPGPQQ-C, from the N Terminus of the protein sequence according to NP_003097.1.
• Peptide Sequence: YNMMETELKPPGPQQ
• Epitope: N Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.3ug/ml) staining of MCF7 nuclear (A) and U251 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.)

Immunofluorescence (IF)

(Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained wit)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fol)

References

Palmqvist L, Glover CH, Hsu L, Lu M, Bossen B, Piret JM, Humphries RK, Helgason CD.: Correlation of murine embryonic stem cell gene expression profiles with functional measures of pluripotency. Stem Cells. 2005 May;23(5):663-80. PMID: 15849174

NCBI and Uniprot Product Information

• NCBI GI #: 28195386
• NCBI GeneID: 6657
• NCBI Accession #: NP_003097.1
• NCBI GenBank Nucleotide #: NP_003097.1
• UniProt Accession #: P48431
• NCBI Official Full Name: transcription factor SOX-2
• NCBI Official Synonym Full Names: SRY-box transcription factor 2
• NCBI Official Symbol: SOX2
• NCBI Official Synonym Symbols: ANOP3; MCOPS3
• NCBI Protein Information: transcription factor SOX-2
• UniProt Protein Name: Transcription factor SOX-2
• UniProt Gene Name: SOX2
• UniProt Entry Name: SOX2_HUMAN

NCBI Description

This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq, Jul 2008]