Mouse anti-Mouse SOS1 Monoclonal Antibody | anti-SOS1 antibody

MOUSE ANTI SOS1

Cat. Num. AAA217406

• Gene Names: SOS1; GF1; HGF; NS4; GGF1; GINGF
• Reactivity: Mouse
• Applications: ELISA, Immunofluorescence, Western Blot
• Synonyms: SOS1; Monoclonal Antibody; MOUSE ANTI SOS1; anti-SOS1 antibody

• Host: Mouse
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: SOS-01
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 1333

• Applicable Applications for anti-SOS1 antibody: ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 150kDa in K562 and RAJI human cell lysates under reducing conditions.; Western Blotting: Maximum Dilution: 1.0ug/ml
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3) ; Preparation
• Buffer Solution: Phosphate buffered saline
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(Western blotting analysis of human SOS1 epression on K562 (lane 1) and Raji (lane 2) cell lysates usingMouse anti Human SOS1 antibody, detection is by chemiluminescence)

Testing Data

(Immunofluorescence analysis of human SOS1 expression (green) on human fibroblasts using Mouse anti Human SOS1, cells are counterstained for actin (red) and DNA using DAPI (blue))

Testing Data

(Immunofluorescence analysis of human SOS1 expression (red) on transformed murine fibroblasts using Mouse anti Human SOS1. Actin is stained green and nuclei (DAPI) blue)

Related Product Information for anti-SOS1 antibody

Mouse anti Human SOS1 antibody, clone SOS-1 recognizes SOS1 (son of sevenless homolog 1), a ubiquitously expressed 1333 amino acid ~150kDa intracellular protein and bifunctional guanine nucleotide exchange factor (GEF), which plays a role in growth and differentiation signalling pathways and in actin reorganization, by acting as a regulator of Ras and Rac/Rho GTPases. Mutations in the SOS1 gene are responsible for the rare oral overgrowth condition known as gingival fibromatosis 1 (Hart et al. 2002), and also the cause of the relatively common autosomal dominant disorder Noonan syndrome type 4 (NS4), characterized by short stature, congenital heart malformation, bleeding diathesis and distinctive facial features (Hanna et al. 2009).

NCBI and Uniprot Product Information

• NCBI GI #: 15529996
• NCBI GeneID: 6654
• NCBI Accession #: NP_005624.2
• NCBI GenBank Nucleotide #: NM_005633.3
• UniProt Accession #: Q07889; A8K2G3; B4DXG2
• Molecular Weight: 36,073 Da
• NCBI Official Full Name: son of sevenless homolog 1
• NCBI Official Synonym Full Names: son of sevenless homolog 1 (Drosophila)
• NCBI Official Symbol: SOS1
• NCBI Official Synonym Symbols: GF1; HGF; NS4; GGF1; GINGF
• NCBI Protein Information: son of sevenless homolog 1; SOS-1; gingival fibromatosis, hereditary, 1; guanine nucleotide exchange factor
• UniProt Protein Name: Son of sevenless homolog 1
• Protein Family: Son of sevenless
• UniProt Gene Name: SOS1
• UniProt Synonym Gene Names: SOS-1
• UniProt Entry Name: SOS1_HUMAN

NCBI Description

This gene encodes a protein that is a guanine nucleotide exchange factor for RAS proteins, membrane proteins that bind guanine nucleotides and participate in signal transduction pathways. GTP binding activates and GTP hydrolysis inactivates RAS proteins. The product of this gene may regulate RAS proteins by facilitating the exchange of GTP for GDP. Mutations in this gene are associated with gingival fibromatosis 1 and Noonan syndrome type 4. [provided by RefSeq, Jul 2008]

Uniprot Description

SOS1: a guanine nucleotide exchange factor that activates Ras by catalyzing the exchange of bound GDP for GTP. Interacts with GRB2, NCK, and other adapter proteins. Plays an important role in signaling pathways leading from the G-protein- coupled receptors and receptor tyrosine kinases to MAPK/ERK.

Protein type: GEFs; GEFs, Ras; Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 2p21

Cellular Component: cell soma; postsynaptic density; plasma membrane; cytosol

Molecular Function: protein binding; Rho guanyl-nucleotide exchange factor activity; DNA binding; protein heterodimerization activity; SH3 domain binding; Ras guanyl-nucleotide exchange factor activity

Biological Process: epidermal growth factor receptor signaling pathway; platelet activation; axon guidance; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; signal transduction; positive regulation of small GTPase mediated signal transduction; regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; positive regulation of epidermal growth factor receptor signaling pathway; Ras protein signal transduction; insulin receptor signaling pathway; innate immune response; blood coagulation; leukocyte migration

Disease: Fibromatosis, Gingival, 1; Noonan Syndrome 4