Search results for " stem cells "

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Immunohistochemistry (IHC) - Paraffin (Dilution: IHC-P - 1:25Immunohistochemical analysis of CD276 Antibody in MCF-7 cell sections. Cell was fixed with formaldehyde and blocked with super block for 10 minutes at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:25) for 1 hours at room temperature. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)

CD276, Monoclonal Antibody (Cat# AAA9231332)

Full Name

CD276 Antibody

Gene Names

CD276; B7H3; B7-H3; B7RP-2; 4Ig-B7-H3

Reactivity

Human

Applications

IHC, WB, EIA

Purity

Purified through a protein G column, followed by dialysis against PBS.

Pricing

$190/0.05 mL | $425/0.2 mL | $1,665/5x0.2 mL

Activity Data (Figure. Cell proliferation of Raw264.7 cells after stimulated with SCF.Stem cell factor (also known as SCF, KIT-ligand, KL, or steel factor) is a cytokine that binds to the c-KIT receptor (CD117). SCF can exist both as a transmembrane protein and a soluble protein. This cytokine plays an important role in hematopoiesis (formation of blood cells), spermatogenesis, and melanogenesis. SCF has been shown to stimulate the proliferation of Raw264.7 cells. To test this effect, Raw264.7 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach later, replaced with serum-free overnight, then the medium was replaced with 2% serum standard DMEM containing various concentrations of recombinant mouse SCF. After incubated for 120h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Proliferation of Raw264.7 cells after incubation with SCF for 120h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant SCF for 120h. The result was shown in Figure 2. It was obvious that SCF significantly increased cell viability of Raw264.7 cells.(A) Raw264.7 cells cultured in DMEM, stimulated with 1ng/mL SCF for 120h; (B) Unstimulated Raw264.7 cells cultured in DMEM for 120h.)

Stem Cell Factor (SCF), Active Protein (Cat# AAA2105078)

Full Name

Active Stem Cell Factor (SCF)

Gene Names

Kitl; Gb; SF; Sl; Clo; Con; Mgf; SCF; SLF; blz; Kitlg; contrasted

Applications

Cell Culture, Activity Assays

Purity

> 97%

Pricing

$230/0.01 mg | $500/0.05 mg | $770/0.1 mg | $955/0.2 mg | $1,860/0.5 mg | $2,755/1 mg | $6,740/5 mg

CD31 / PECAM-1, Monoclonal Antibody (Cat# AAA438300)

Full Name

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

Gene Names

PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM

Reactivity

Human

Applications

FC/FACS, IF, IHC

Pricing

$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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Cytokeratin 19 (KRT19), Monoclonal Antibody (Cat# AAA438383)

Full Name

Cytokeratin 19 (KRT19) (Pancreatic Stem Cell Marker) Mouse Monoclonal Antibody

Gene Names

KRT19; K19; CK19; K1CS

Reactivity

Human.
Does not react with Rat and Ferret

Applications

FC/FACS, IF, IHC

Pricing

$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)

CD33, Monoclonal Antibody (Cat# AAA438398)

Full Name

CD33

Gene Names

CD33; p67; SIGLEC3; SIGLEC-3

Reactivity

Human.

Applications

EIA, FC/FACS, IF, IP, IH

Pricing

$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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Western Blot (WB) (Western blot analysis of extracts of various celllines, using TERT antibody.)

TERT, Antibody (Cat# AAA8500687)

Full Name

TERT Antibody

Gene Names

TERT; TP2; TRT; CMM9; EST2; TCS1; hTRT; DKCA2; DKCB4; hEST2; PFBMFT1

Applications

WB, IHC

Purity

Affinity purification

Pricing

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Western Blot (WB) (Figure 1. Western blot analysis using WNT1 mouse mAb against NIH/3T3 (1), 3T3L1 (2) and Hela (3) cell lysate.)

WNT1, Monoclonal Antibody (Cat# AAA850112)

Full Name

WNT1 Antibody

Gene Names

WNT1; INT1; OI15; BMND16

Reactivity

Human, Mouse

Applications

WB, IHC, IF, FC/FACS, EIA

Pricing

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TrkA, Polyclonal Antibody (Cat# AAA9600757)

Full Name

Phospho-TrkA (Tyr490) Antibody

Gene Names

NTRK1; MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA

Reactivity

Human, Mouse, Rat

Applications

WB, IHC

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$260/0.1 mL | $310/0.2 mL | $1,330/5x0.2 mL

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Western Blot (WB) (Western Blot analysis of 1) Hela, 2) Mouse brain, 3) Rat brain using ACTA1 Monoclonal Antibody at dilution of 1:1000.)

alpha skeletal muscle actin, Monoclonal Antibody (Cat# AAA2534216)

Full Name

alpha skeletal muscle actin Monoclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, IF, IP

Purity

Protein A purification

Pricing

$125/0.02 mL | $405/0.2 mL | $255/0.12 mL | $180/0.06 mL | $1,825/5x0.2 mL

PAX6, Monoclonal Antibody (Cat# AAA438437)

Full Name

PAX6 (Stem Cell Marker) Mouse Monoclonal Antibody

Gene Names

PAX6; AN; AN2; FVH1; MGDA; WAGR; D11S812E

Reactivity

Human, Mouse, Rat, Chicken

Applications

FC/FACS, IF, WB

Pricing

$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)

CD31 / PECAM-1, Monoclonal Antibody (Cat# AAA438783)

Full Name

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

Gene Names

PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM

Reactivity

Human, Monkey, Rabbit

Applications

FC/FACS, IF

Purity

Purified Ab conjugated to PE

Pricing

$390/0.5 mL | $1,710/5x0.5 mL

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Western Blot (WB) (Figure 1: Western blot analysis using SMN1 mouse mAb against HepG2 (1), Hela (2), K562 (3), Jurkat (4), SKBR-3 (5), A431 (6) and Cos7 (7) cell lysate.)

SMN1, Antibody (Cat# AAA850993)

Full Name

SMN1 Antibody

Gene Names

SMN1; SMA; SMN; SMA1; SMA2; SMA3; SMA4; SMA@; SMNT; BCD541; GEMIN1; TDRD16A; T-BCD541

Reactivity

Human, Monkey

Applications

WB, IHC, FC/FACS, EIA

Pricing

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Testing Data (P-peptide-+)

C-RAF, Antibody (Cat# AAA855371)

Full Name

C-RAF (Phospho-Ser621) Antibody

Gene Names

RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, EIA

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

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Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human kidney stained with CD31 Mouse Monoclonal Antibody (PECAM1/3528).)

CD31/PECAM-1, Monoclonal Antibody (Cat# AAA4381157)

Full Name

CD31/PECAM-1 (Endothelial Cell Marker)

Gene Names

PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM

Reactivity

Human

Applications

IHC

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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Cytokeratin 15, Monoclonal Antibody (Cat# AAA4381055)

Full Name

Cytokeratin 15 (Esophageal Squamous Cell Carcinoma Marker)

Gene Names

KRT15; K15; CK15; K1CO

Reactivity

Human

Applications

FC/FACS, IF, WB, IHC

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 19 Monoclonal Antibody (BA17))

Cytokeratin 19 (KRT19), Monoclonal Antibody (Cat# AAA438442)

Full Name

Cytokeratin 19 (KRT19) (Pancreatic Stem Cell Marker) Mouse Monoclonal Antibody

Gene Names

KRT19; K19; CK19; K1CS

Reactivity

Human, Mouse

Applications

FC, IF, IHC

Pricing

$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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Western Blot (WB) (Western blot analysis of lysate from HT29 cell line, using KLF4 Antibody. MBS9212050 was diluted at 1:1000. A goat anti-rabbit (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)

KLF4, Polyclonal Antibody (Cat# AAA9212050)

Full Name

KLF4 Antibody

Gene Names

KLF4; EZF; GKLF

Reactivity

Human

Applications

IF, EIA, IHC, WB

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL

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Immunohistochemistry (IHC) (Peptide: - / +Immunohistochemistry analysis of paraffin-embedded human brain tissue using MRC2 antibody.)

MRC2, Antibody (Cat# AAA853311)

Full Name

MRC2 Antibody

Gene Names

MRC2; CD280; UPARAP; CLEC13E; ENDO180

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, EIA

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

CD34, Monoclonal Antibody (Cat# AAA438123)

Full Name

CD34

Reactivity

Human and Rat. Others not known.

Applications

FC/FACS

Purity

Purified Ab conjugated to PE

Pricing

$390/0.5 mL | $1,710/5x0.5 mL

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Cytokeratin 19, Monoclonal Antibody (Cat# AAA4380082)

Full Name

Cytokeratin 19 (Pancreatic Stem Cell Marker)

Gene Names

KRT19; K19; CK19; K1CS

Reactivity

Human, Mouse, Rat. Others not known.

Applications

FC/FACS, IF, IHC

Pricing

$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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Cytokeratin 15 (KRT15), Monoclonal Antibody (Cat# AAA4380074)

Full Name

Cytokeratin 15 (KRT15) (Basal Cell Marker)

Gene Names

KRT15; K15; CK15; K1CO

Reactivity

Human, Rat, Cow. Others not known.

Applications

FC/FACS, IF, WB, IHC

Pricing

$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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Testing Data (Published customer image: Direct detection of the M42 polypeptide. (A) Validation of anti-M42 serum. Lysates from cells transfected with the indicated GFP polypeptides were analysed by SDS-PAGE and western blotting as labeled. (B) Detection of M42 from virus-infected cells. Lysates from cells infected with the indicated viruses at 10 h p.i. were analysed by SDS-PAGE and western blotting as labeled. The same membrane was probed with mouse anti-M2 14C2 and rabbit anti-M42 using different colour secondary antisera; individual grey scale and colour merged images are shown.From: Wise HM, Hutchinson EC, Jagger BW, Stuart AD, Kang ZH, et al. (2012) Identification of a Novel Splice Variant Form of the Influenza A Virus M2 Ion Channel with an Antigenically Distinct Ectodomain. PLoS Pathog 8(11): e1002998.)

Testing Data (Published customer image: Expression of PpiMHCIIa, PpiMHCIIb1 and PpiMHCIIb2 in the tentacular apparatus. (A) Schematic drawing of the tentacle root in internal view. The three coloured horizontal lines materialise the three different planes of transverse cryosections corresponding to panels D, I, N (in green), E, J, O (in orange) and F, K, P (in purple). (A') The tentacle root in external view (corresponding to the side of tentacle insertion). The dotted lines delineate the longitudinal dissections to remove tentacle root lateral expansions in order to obtain the preparations shown in (C, H, M). (A) Schematic drawing of the tentacle root in longitudinal section, after removal of the lateral expansions following the dotted lines in (A'). Horizontal lines indicating the three planes of cryosections as in (A). (B, G, L) Internal views of isolated tentacle roots showing PpiMHCIIa (B), PpiMHCIIb1 (G), PpiMHCIIb2 (L) expressions. (C, H, M) Longitudinal sections of the tentacle root stained with PpiMHCIIa (C), PpiMHCIIb1 (H), PpiMHCIIb2 (M) anti-sense probes. The aboral pole is at the top in panels (B, C, G, H, L, M). (D-F, I-K, N-P) Transverse cryosections of whole-mount ISH for the three genes, with sectioning plane indicated by the colour of the surrounding line according to the colour code outlined in panel (A). The black arrowhead in (G-J) points to a stained line above the median ridge which appears to be more or less in continuity with the two layered bands labelled M Tcl. (Q) YL1/2 (anti-tyrosylated-a-tubulin, in red) and DAPI (in blue) counterstaining of the region boxed in (N). (R) Higher magnification of the region indicated by the box in (Q). (S) YL1/2 (red) and DAPI (blue) counterstaining of the region boxed in (P). (T-W) Expression of PpiMHCIIb1 gene in tentillae. (T) Whole mount ISH of tentacle and tentillae. (U) Higher magnification view of the region boxed in (T). (V) Transverse cryosection of whole-mount ISH of a tentilla. Two symmetrical muscle fibres are stained. (W) YL1/2 (in red) and DAPI (in blue) counterstaining of (V). The YL1/2 staining in colloblasts is certainly due to non-specific fixation of the antibody on the sticky colloblast granules. (X) Schematic drawing of a tentilla in transverse section. Coll: Colloblasts; F tt: Forming tentillae; LR: Lateral Ridge; MR: Median Ridge; M tcl: Tentacle Muscle progenitors; M tt: Tentilla muscle progenitors; Mu: Muscle fibres; N Tcl: Tentacle Neural cells; N tt: Tentilla Neural cells Tcl: Tentacle; Tt: Tentilla. Scale bars: B, C, G, H, L, M: 100 um; D-F, I-K, N-P: 200 um; Q: 100 um; R, S: 10 um; T: 50 um; U, V, W: 25 um.From: Independent specialisation of myosin II paralogues in muscle vs. non-muscle functions during early animal evolution: a ctenophore perspective. Dayraud, CC. et al. BMC Evolutionary Biology 2012, 12:107.)

TUBULIN ALPHA, Monoclonal Antibody (Cat# AAA219562)

Full Name

RAT ANTI TUBULIN ALPHA:HRP

Applications

EIA, WB

Pricing

$425/0.1 mg | $1,755/5x0.1 mg

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Western Blot (WB) (VITRN Antibody (C-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the VITRN antibody detected the VITRN protein (arrow).)

VITRN, Polyclonal Antibody (Cat# AAA9205961)

Full Name

VITRN Antibody (C-term)

Reactivity

Human
Predicted Reactivity: Bovine, Mouse

Applications

WB, EIA, IHC, FC

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL

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Mitochondria, Monoclonal Antibody (Cat# AAA438169)

Full Name

Mitochondria (Marker for Human Cells) Mouse Monoclonal Antibody

Reactivity

Human.
Does not react with Mouse and Rat

Applications

IF, IHC

Pricing

$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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Western Blot (WB) (Western Blot analysis of Rat liver, Mouse spleen and Mouse liver using IL6R Polycloanl Antibody at dilution of 1:2000)

IL6R, Polyclonal Antibody (Cat# AAA2561896)

Full Name

IL6R Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

WB, IHC

Purity

Antigen Affinity Purification

Pricing

$120/0.02 mL | $370/0.2 mL | $245/0.12 mL | $165/0.06 mL | $1,665/5x0.2 mL

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GFAP, Monoclonal Antibody (Cat# AAA439986)

Full Name

GFAP (Astrocyte & Neural Stem Cell Marker)

Gene Names

GFAP; ALXDRD

Reactivity

Human, Mouse, Rat, Cow, Pig, Rabbit, Chicken. Others not known.

Applications

FC/FACS, IF, WB, IHC

Pricing

$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)

Podocalyxin (PODXL), Monoclonal Antibody (Cat# AAA439444)

Full Name

Podocalyxin (PODXL) (Hematopoietic Stem Cell Marker) Mouse Monoclonal Antibody

Gene Names

PODXL; PC; PCLP; Gp200; PCLP-1

Reactivity

Human, Rabbit, Rat

Applications

FC/FACS, IF, IHC

Pricing

$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA at 1mg/mL)

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Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin 15 Rabbit Recombinant Monoclonal Ab (KRT15/2103R).)

Cytokeratin 15 (KRT15), Monoclonal Antibody (Cat# AAA4380078)

Full Name

Cytokeratin 15 (KRT15) (Basal Cell Marker)

Gene Names

KRT15; K15; CK15; K1CO

Reactivity

Human, Rat, Cow. Others not known.

Applications

IHC

Pricing

$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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Testing Data

CD130/gp130, Antibody (Cat# AAA859145)

Full Name

CD130/gp130 (Ab-782) Antibody

Gene Names

IL6ST; CD130; GP130; CDW130; IL-6RB

Reactivity

Human, Mouse

Applications

WB, IHC, IF, EIA

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

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SOX2, Monoclonal Antibody (Cat# AAA4380226)

Full Name

SOX2 (Transcription Factor)

Gene Names

SOX2; ANOP3; MCOPS3

Reactivity

Human. Others not known.

Applications

EIA, FC/FACS, IF, WB, IHC

Pricing

$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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Activity Data (Platelet Derived Growth Factor Subunit A (PDGFA) is a member of Platelet-derived growth factor (PDGF) family which plays a significant role in blood vessel formation, the growth of blood vessels from already-existing blood vessel tissue, mitogenesis, i.e. proliferation, of mesenchymal cells such as fibroblasts, osteoblasts, tenocytes, vascular smooth muscle cells and mesenchymal stem cells as well as chemotaxis, the directed migration, of mesenchymal cells. Besides, Platelet Derived Growth Factor Receptor Alpha (PDGFRa) has been identified as an interactor of PDGFA, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PDGFA and recombinant human PDGFRa. Briefly, PDGFA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100mul were then transferred to PDGFRa-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-PDGFA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of PDGFA and PDGFRa was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of PDGFA with PDGFRa.)

Platelet Derived Growth Factor Subunit A (PDGFA), Active Protein (Cat# AAA2105097)

Full Name

Active Platelet Derived Growth Factor Subunit A (PDGFA)

Gene Names

PDGFA; PDGF1; PDGF-A

Applications

Cell Culture, Activity Assays

Purity

> 90%

Pricing

$225/0.01 mg | $475/0.05 mg | $735/0.1 mg | $910/0.2 mg | $1,775/0.5 mg | $2,625/1 mg | $6,415/5 mg

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Western Blot (WB) (Figure 1: Western blot analysis using PDK1 mouse mAb against NIH/3T3 (1), Hela (2), Jurkat (3), HepG2 (4), PC-12 (5), and Cos7 (6) cell lysate.)

PDK1, Antibody (Cat# AAA850452)

Full Name

PDK1 Antibody

Reactivity

Human, Monkey, Rat

Applications

WB, IHC, IF, FC/FACS, EIA

Pricing

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Testing Data (Peptide-+)

TNFRSF6B, Antibody (Cat# AAA852455)

Full Name

TNFRSF6B Antibody

Gene Names

TNFRSF6B; M68; TR6; DCR3; M68E; DJ583P15.1.1

Reactivity

Human

Applications

WB, IHC, IF, EIA

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

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Flow Cytometry (FC/FACS) ( Flow-cytometry using anti-CD34 antibody My10 Human leukocytes were stained with an isotype control or the rabbit-chimeric version of My10 at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)

CD34, Monoclonal Recombinant Antibody (Cat# AAA488220)

Full Name

Anti-CD34 [My10]

Reactivity

Human

Applications

FC/FACS, IP, IHC

Purity

Purified
Protein A Affinity Purified

Pricing

$495/0.2 mg | $2,025/5x0.2 mg

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ISG15, Polyclonal Antibody (Cat# AAA3214314)

Full Name

ISG15 antibody - middle region

Gene Names

ISG15; G1P2; IP17; UCRP; IFI15; IMD38; hUCRP

Reactivity

Tested Reactivity: Human, Mouse
Predicted Reactivity: Cow, Dog, Goat, Horse, Human, Pig, Rabbit, Sheep

Applications

IHC, WB

Purity

Affinity Purified

Pricing

$445/0.1 mL | $1,985/5x0.1 mL

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CD38, Antibody (Cat# AAA850127)

Full Name

CD38 Antibody

Gene Names

CD38; ADPRC1; ADPRC 1

Reactivity

Human

Applications

WB, IHC, EIA

Pricing

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Immunohistochemistry (IHC) (Peptide-+ Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Ku80 (Ab-714) Antibody.)

Ku80, Antibody (Cat# AAA859388)

Full Name

Ku80 (Ab-714) Antibody

Gene Names

XRCC5; KU80; KUB2; Ku86; NFIV; KARP1; KARP-1

Reactivity

Human, Mouse

Applications

WB, IHC, IF, EIA

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

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GFAP, Monoclonal Antibody (Cat# AAA439987)

Full Name

GFAP (Astrocyte & Neural Stem Cell Marker)

Gene Names

GFAP; ALXDRD

Reactivity

Human, Mouse, Rat, Cow, Pig, Rabbit, Chicken. Others not known.

Applications

FC/FACS, IF, WB, IHC

Pricing

$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)

CD31 / PECAM-1, Monoclonal Antibody (Cat# AAA438712)

Full Name

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

Gene Names

PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM

Reactivity

Human

Applications

FC/FACS, IF

Pricing

$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)

CD117 / c-Kit, Monoclonal Antibody (Cat# AAA439563)

Full Name

CD117 / c-Kit (Marker for Gastrointestinal Stromal Tumors) Mouse Monoclonal Antibody

Gene Names

KIT; PBT; SCFR; C-Kit; CD117

Reactivity

Human

Applications

FC/FACS, IF, IHC

Pricing

$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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Western Blot (WB) (Figure 1. Western blot analysis of GAS 6 using anti-GAS 6 antibody (MBS1750446).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysates,Lane 2: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAS 6 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GAS 6 at approximately 75KD. The expected band size for GAS 6 is at 79KD. )

GAS 6, Polyclonal Antibody (Cat# AAA1750446)

Full Name

Anti-GAS 6 Picoband Antibody

Gene Names

GAS6; AXSF; AXLLG

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

IHC, WB

Purity

Immunogen affinity purified

Pricing

$345/0.1 mg | $1,485/5x0.1 mg

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Western Blot (WB) (Western blot analysis of extracts from L-540 cells, using Phospho-TNK1 (Tyr277) Antibody. Lane1 was treated with phospho-blocking peptide, Lane 2 was treated with non-phospho-blocking peptide.)

TNK1, Polyclonal Antibody (Cat# AAA9600754)

Full Name

Phospho-TNK1 (Tyr277) Antibody

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, EIA

Purity

Peptide affinity purification

Pricing

$260/0.1 mL | $310/0.2 mL | $1,330/5x0.2 mL

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Western Blot (WB) (Western blot analysis of extracts of Mouse braintissue lysate, using KISS1antibody.)

KISS1, Antibody (Cat# AAA8503485)

Full Name

KISS1 Antibody

Gene Names

KISS1; HH13; KiSS-1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC

Purity

Affinity purification

Pricing

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Western Blot (WB) (Figure 1. Western blot analysis using STYK1 mouse mAb against truncated STYK1 recombinant protein(1) and STYK1 (aa47-422)-hIgGFc transfected CHO-K1 cell lysate (2).)

RET, Antibody (Cat# AAA850428)

Full Name

RET Antibody

Gene Names

RET; PTC; MTC1; HSCR1; MEN2A; MEN2B; CDHF12; CDHR16; RET-ELE1

Reactivity

Human

Applications

WB, EIA

Pricing

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Immunohistochemistry (IHC) (P-peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 14-3-3 zeta (phospho-Ser58) antibody.)

14-3-3 zeta, Antibody (Cat# AAA851199)

Full Name

14-3-3 zeta (Phospho-Ser58) Antibody

Gene Names

YWHAZ; HEL4; YWHAD; KCIP-1; HEL-S-3; HEL-S-93; 14-3-3-zeta

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, EIA

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

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Testing Data (P-peptide-+)

AurB, Antibody (Cat# AAA859011)

Full Name

AurB (Phospho-Thr232) Antibody

Gene Names

AURKB; AIK2; AIM1; ARK2; AurB; IPL1; STK5; AIM-1; ARK-2; STK-1; STK12; PPP1R48; aurkb-sv1; aurkb-sv2

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, EIA

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

CD33, Monoclonal Antibody (Cat# AAA438512)

Full Name

CD33

Gene Names

CD33; p67; SIGLEC3; SIGLEC-3

Reactivity

Human.

Applications

FC/FACS

Purity

Purified Ab conjugated to PE

Pricing

$390/0.5 mL | $1,710/5x0.5 mL

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Western Blot (WB) (Figure 1.Western blot analysis using NPM mouse mAb against SMMC-7721 (1), HepG2 (2), Hela (3) and HEK293 (4) cell lysate.)

Nucleophosmin, Antibody (Cat# AAA850728)

Full Name

Nucleophosmin Antibody

Gene Names

NPM1; B23; NPM

Reactivity

Human, Monkey

Applications

WB, IHC, IF, EIA

Pricing

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Testing Data (Figure 1: MUB1900P: Immunohistochemistry on frozen section of swine colon showing positive staining in connective tissue cells and no reactivity in epithelial cells )

Vimentin, Monoclonal Antibody (Cat# AAA570247)

Full Name

Mouse anti Vimentin, conjugated with HRP

Gene Names

VIM; CTRCT30

Reactivity

Canine, Chicken, Caprine, Hamster, Human, Monkey, Mouse, Rat, Swine, Zebrafish

Applications

FC, IHC, IHC, frozen, IHC, paraffin, WB

Pricing

$585/1 mL | $2,485/5x1 mL

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Immunohistochemistry (IHC)-Paraffin (Staining Scx in Mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)

Scx, Polyclonal Antibody (Cat# AAA9232035)

Full Name

(Mouse) Scx Antibody (N-term)

Gene Names

Scx; Scl; Bhlha41; BB114693

Reactivity

Mouse
Predicted: Human

Applications

WB, IHC, EIA

Pricing

$425/0.1 mL | $1,615/5x0.1 mL

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Isotype

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Application

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10000 results for " stem cells " - showing 300-350

CD276, Monoclonal Antibody (Cat# AAA9231332)

Stem Cell Factor (SCF), Active Protein (Cat# AAA2105078)

CD31 / PECAM-1, Monoclonal Antibody (Cat# AAA438300)

Cytokeratin 19 (KRT19), Monoclonal Antibody (Cat# AAA438383)

CD33, Monoclonal Antibody (Cat# AAA438398)

TERT, Antibody (Cat# AAA8500687)

WNT1, Monoclonal Antibody (Cat# AAA850112)

TrkA, Polyclonal Antibody (Cat# AAA9600757)

alpha skeletal muscle actin, Monoclonal Antibody (Cat# AAA2534216)

PAX6, Monoclonal Antibody (Cat# AAA438437)

CD31 / PECAM-1, Monoclonal Antibody (Cat# AAA438783)

SMN1, Antibody (Cat# AAA850993)

C-RAF, Antibody (Cat# AAA855371)

CD31/PECAM-1, Monoclonal Antibody (Cat# AAA4381157)

Cytokeratin 15, Monoclonal Antibody (Cat# AAA4381055)

Cytokeratin 19 (KRT19), Monoclonal Antibody (Cat# AAA438442)

KLF4, Polyclonal Antibody (Cat# AAA9212050)

MRC2, Antibody (Cat# AAA853311)

CD34, Monoclonal Antibody (Cat# AAA438123)

Cytokeratin 19, Monoclonal Antibody (Cat# AAA4380082)

Cytokeratin 15 (KRT15), Monoclonal Antibody (Cat# AAA4380074)

TUBULIN ALPHA, Monoclonal Antibody (Cat# AAA219562)

VITRN, Polyclonal Antibody (Cat# AAA9205961)

Mitochondria, Monoclonal Antibody (Cat# AAA438169)

IL6R, Polyclonal Antibody (Cat# AAA2561896)

GFAP, Monoclonal Antibody (Cat# AAA439986)

Podocalyxin (PODXL), Monoclonal Antibody (Cat# AAA439444)

Cytokeratin 15 (KRT15), Monoclonal Antibody (Cat# AAA4380078)

CD130/gp130, Antibody (Cat# AAA859145)

SOX2, Monoclonal Antibody (Cat# AAA4380226)

Platelet Derived Growth Factor Subunit A (PDGFA), Active Protein (Cat# AAA2105097)

PDK1, Antibody (Cat# AAA850452)

TNFRSF6B, Antibody (Cat# AAA852455)

CD34, Monoclonal Recombinant Antibody (Cat# AAA488220)

ISG15, Polyclonal Antibody (Cat# AAA3214314)

CD38, Antibody (Cat# AAA850127)

Ku80, Antibody (Cat# AAA859388)

GFAP, Monoclonal Antibody (Cat# AAA439987)

CD31 / PECAM-1, Monoclonal Antibody (Cat# AAA438712)

CD117 / c-Kit, Monoclonal Antibody (Cat# AAA439563)

GAS 6, Polyclonal Antibody (Cat# AAA1750446)

TNK1, Polyclonal Antibody (Cat# AAA9600754)

KISS1, Antibody (Cat# AAA8503485)

RET, Antibody (Cat# AAA850428)

14-3-3 zeta, Antibody (Cat# AAA851199)

AurB, Antibody (Cat# AAA859011)

CD33, Monoclonal Antibody (Cat# AAA438512)

Nucleophosmin, Antibody (Cat# AAA850728)

Vimentin, Monoclonal Antibody (Cat# AAA570247)

Scx, Polyclonal Antibody (Cat# AAA9232035)

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