Western Blot (WB)
(Figure 1. Western blot analysis using WNT1 mouse mAb against NIH/3T3 (1), 3T3L1 (2) and Hela (3) cell lysate.)
Mouse anti-Human, Mouse WNT1 Monoclonal Antibody | anti-WNT1 antibody
WNT1 Antibody
IHC: 1:200-1:1000
IF: 1:200-1:1000
FC: 1:200-1:400
ELISA Propose dilution: 1:10000
Not yet tested in other applications. Determining optimal working dilutions by titration test.
Western Blot (WB)
(Figure 1. Western blot analysis using WNT1 mouse mAb against NIH/3T3 (1), 3T3L1 (2) and Hela (3) cell lysate.)
Western Blot (WB)
(Figure 1. Western blot analysis using WNT1 mouse mAb against NIH/3T3 (1), 3T3L1 (2) and Hela (3) cell lysate.)
Immunohistochemistry (IHC)
(Figure 2. Immunohistochemical analysis of paraffin-embedded human LAdrenal tissues using WNT1 mouse mAb)
Immunohistochemistry (IHC)
(Figure 2. Immunohistochemical analysis of paraffin-embedded human LAdrenal tissues using WNT1 mouse mAb)
Immunofluorescence (IF)
(Figure3. Confocal immunofluorescence analysis of Hela (left) and 3T3-L1 (right) cells using WNT1 mouse mAb (green). Red. Actin filaments have been labeled with DY-554 phalloidin. Blue. DRAQ5 fluorescent DNA dye.)
Immunofluorescence (IF)
(Figure3. Confocal immunofluorescence analysis of Hela (left) and 3T3-L1 (right) cells using WNT1 mouse mAb (green). Red. Actin filaments have been labeled with DY-554 phalloidin. Blue. DRAQ5 fluorescent DNA dye.)
NCBI and Uniprot Product Information
NCBI Description
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It is very conserved in evolution, and the protein encoded by this gene is known to be 98% identical to the mouse Wnt1 protein at the amino acid level. The studies in mouse indicate that the Wnt1 protein functions in the induction of the mesencephalon and cerebellum. This gene was originally considered as a candidate gene for Joubert syndrome, an autosomal recessive disorder with cerebellar hypoplasia as a leading feature. However, further studies suggested that the gene mutations might not have a significant role in Joubert syndrome. This gene is clustered with another family member, WNT10B, in the chromosome 12q13 region. [provided by RefSeq, Jul 2008]
Uniprot Description
Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Acts in the canonical Wnt signaling pathway by promoting beta-catenin-dependent transcriptional activation (PubMed:23499309, PubMed:26902720, PubMed:28528193, PubMed:23656646). In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling (). Plays an essential role in the development of the embryonic brain and central nervous system (CNS) (). Has a role in osteoblast function, bone development and bone homeostasis (PubMed:23499309, PubMed:23656646).
Research Articles on WNT1
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Product Notes
The WNT1 wnt1 (Catalog #AAA850112) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The WNT1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's WNT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:200-1:1000 IF: 1:200-1:1000 FC: 1:200-1:400 ELISA Propose dilution: 1:10000 Not yet tested in other applications. Determining optimal working dilutions by titration test. Researchers should empirically determine the suitability of the WNT1 wnt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "WNT1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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