Rabbit anti-Human, Mouse ATG12 Polyclonal Antibody | anti-ATG12 antibody

ATG12, NT (APG12, APG12L, HAPG12, APG12 Autophagy 12-like, APG12-like, Apg12 (Autophagy, yeast) Homolog, Autophagy-related Protein 12) (PE)

Cat. Num. AAA6463477

• Gene Names: ATG12; APG12; FBR93; APG12L; HAPG12
• Reactivity: Human, Mouse
• Applications: Immunofluorescence, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: ATG12; Polyclonal Antibody; NT (APG12; APG12L; HAPG12; APG12 Autophagy 12-like; APG12-like; Apg12 (Autophagy; yeast) Homolog; Autophagy-related Protein 12) (PE); anti-ATG12 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human APG12L. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ATG12 antibody: Immunofluorescence (IF), FLISA, Western Blot (WB)
• Application Notes: IF: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human APG12L (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of hAPG12L-T23 in MCF-7 cell line lysates (35ug/lane). APG12L (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of hAPG12L-T23 in mouse brain tissue lysates (35ug/lane). APG12L (arrow) was detected using the purified Pab.)

Testing Data

(Mouse leukaemic monocyte macrophage cells treated with vinblastine for 1 hr. APG12L antibody detected punctuate staining indicative of nascent autophagic vacuole structures. Data courtesy of Dr. Barry Boland, Department of Pharmacology, Oxford University.)

Product Categories/Family for anti-ATG12 antibody

Antibodies; Autophagy

References

1. Tanida, I., et al., Biochem. Biophys. Res. Commun. 296(5):1164-1170 (2002). 2. Tanida, I., et al., J. Biol. Chem. 277(16):13739-13744 (2002). 3. Mizushima, N., et al., J. Biol. Chem. 273(51):33889-33892 (1998). 4. Ueno, K., et al., Hum. Genet. 102(1):63-68 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 290560746
• NCBI GeneID: 9140
• NCBI Accession #: NP_004698
• NCBI GenBank Nucleotide #: NM_004707
• UniProt Accession #: O94817
• Molecular Weight: 21kDa
• NCBI Official Full Name: ubiquitin-like protein ATG12 isoform 1
• NCBI Official Synonym Full Names: autophagy related 12
• NCBI Official Symbol: ATG12
• NCBI Official Synonym Symbols: APG12; FBR93; APG12L; HAPG12
• NCBI Protein Information: ubiquitin-like protein ATG12
• UniProt Protein Name: Ubiquitin-like protein ATG12
• Protein Family: Autophagy protein
• UniProt Gene Name: ATG12
• UniProt Synonym Gene Names: APG12; APG12L; APG12-like
• UniProt Entry Name: ATG12_HUMAN

NCBI Description

Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM, Mar 2008]

Uniprot Description

ATG12: Ubiquitin-like protein required for autophagy. Conjugated to ATG3 and ATG5. Ubiquitous. Belongs to the ATG12 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: 5q21-q22

Cellular Component: pre-autophagosomal structure membrane

Molecular Function: protein binding; APG8 conjugating enzyme activity

Biological Process: mitochondrion degradation; innate immune response; C-terminal protein lipidation; negative regulation of interferon type I production; cellular response to nitrogen starvation; autophagic vacuole formation